DNA Damage Repair

DNA是合成任意生物的基因信息的载体。DNA中修复的基因编码对于亚细胞放大可见的过程和生物总体功能至关重要。尽管如此，DNA一直受到内来源的损伤，比如：水解、氧化、烷基化或复制错误。另外，电离辐射、紫外线辐射和过多的化学试剂已经成为威胁DNA完整性的外部因素。

与RNA和蛋白不一样的是，DNA受损后不能降解重新合成。而是存在多种修复途径来确保DNA仍然保持完好。在1974年，弗朗西斯·克里克注意到“在DNA修复中，我们完全漏掉了酶可能的作用。我后来意识到DNA很珍贵，因此有可能存在多种不同的机制。

到今天这个预言还是适用：参与到DNA修复途径复杂的网络中后，一百多个基因被表征。DNA损伤可以通过六种不同的途径修复，这取决于损伤的类型：化学改性、未合并的核苷酸，通过直接反转（DR）、错配修复（MMR）和核苷酸切除修复机制反转交联。DNA单链断裂通过基本切除修复改善。高诱变DNA双链断裂最后通过多种复杂的途径修复，这些途径依赖于姐妹染色单体（在细胞周期S或G2阶段）的同源重组（HR）或双链断裂两端的非同源末端接合（NHEJ）。在DNA损伤不能及时修复的情况下，专门的DNA聚合酶启动跨损伤合成（TLS），从而防止DNA复制叉停止。造成这些修复途径的组件不工作的突变会导致疾病，比如：着色性干皮病、共济失调毛细血管扩张、范科尼贫血和患癌症的倾向。

另外，对于目前通常利用细胞DNA修复机制的靶向基因组编辑方法来说，这些修复机制受到高度关注。

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Canonical Non-Homologous End-Joining

PRKDC (Protein Kinase, DNA-Activated, Catalytic Polypeptide):

Immunofluorescent analysis of DNA-PKcs staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Western blot analysis of extracts of various cell lines, using PRKDC antibody.

PRKDC monoclonal antibody (M03), clone 2A8 Western Blot analysis of PRKDC expression in Hela S3 NE .

This gene encodes the catalytic subunit of the DNA-dependent protein kinase (DNA-PK). It functions with the Ku70/Ku80 heterodimer protein in DNA double strand break repair and recombination. The protein encoded is a member of the PI3/PI4-kinase family.[provided by RefSeq, Jul 2010]. 更多...

XRCC6 - X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 6

Western Blot: Ku70/XRCC6 Antibody (7A9E7) - Analysis using XRCC6 mAb against HEK293 (1) and XRCC6 (AA: 6-214)-hIgGFc transfected HEK293 (2) cell lysate.

Western blot analysis of Ku70 (arrow) using rabbit polyclonal Ku70 Antibody (Center) (ABIN652244). 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected with the Ku70 gene (Lane 2).

The p70/p80 autoantigen is a nuclear complex consisting of two subunits with molecular masses of approximately 70 and 80 kDa. The complex functions...

XRCC6 抗体

XRCC6 ELISA试剂盒

XRCC6 蛋白

XRCC5 (X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 5 (Double-Strand-Break Rejoining)):

ELISA: Ku80/XRCC5 Antibody (5C5) [NBP2-37583] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Immunofluorescence analysis of A549 cells, using Ku70/80 Antibody . The picture on the right is treated with the synthesized peptide.

The protein encoded by this gene is the 80-kilodalton subunit of the Ku heterodimer protein which is also known as ATP-dependant DNA helicase II or...

XRCC5 抗体

XRCC5 ELISA试剂盒

XRCC5 蛋白

此分类里的别的抗原：

ATM (Ataxia Telangiectasia Mutated):	ATM 抗体	ATM ELISA试剂盒	ATM 蛋白
TRIM29 (Tripartite Motif Containing 29):	TRIM29 抗体		TRIM29 蛋白
ATR (Ataxia Telangiectasia and Rad3 Related):	ATR 抗体
H2AFX - H2A Histone Family, Member X:	H2AFX 抗体	H2AFX ELISA试剂盒	H2AFX 蛋白
XRCC4 (X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 4):	XRCC4 抗体	XRCC4 ELISA试剂盒	XRCC4 蛋白
LIG4 - Ligase IV, DNA, ATP-Dependent:	LIG4 抗体	LIG4 ELISA试剂盒	LIG4 蛋白
NHEJ1 - Nonhomologous End-Joining Factor 1:	NHEJ1 抗体	NHEJ1 ELISA试剂盒	NHEJ1 蛋白
APTX - Aprataxin:	APTX 抗体	APTX ELISA试剂盒	APTX 蛋白
APLF (Aprataxin and PNKP Like Factor):	APLF 抗体		APLF 蛋白
DCLRE1C (DNA Cross-Link Repair 1C):	DCLRE1C 抗体		DCLRE1C 蛋白
PNKP (Polynucleotide Kinase 3'-Phosphatase):	PNKP 抗体	PNKP ELISA试剂盒	PNKP 蛋白
DNTT - Deoxynucleotidyltransferase, terminal:	DNTT 抗体	DNTT ELISA试剂盒	DNTT 蛋白
POLL (Polymerase (DNA Directed), lambda):	POLL 抗体		POLL 蛋白
POLM - Polymerase (DNA Directed), mu:	POLM 抗体		POLM 蛋白
MRE11A - MRE11 Meiotic Recombination 11 Homolog A (S. Cerevisiae):	MRE11A 抗体	MRE11A ELISA试剂盒	MRE11A 蛋白
RAD50 (RAD50 Homolog (S. Cerevisiae)):	RAD50 抗体	RAD50 ELISA试剂盒	RAD50 蛋白
NBN - Nibrin:	NBN 抗体	NBN ELISA试剂盒	NBN 蛋白
TP53BP1 (Tumor Protein P53 Binding Protein 1):	TP53BP1 抗体	TP53BP1 ELISA试剂盒	TP53BP1 蛋白

Microhomology-Mediated End-Joining

PARP1 (Poly (ADP-Ribose) Polymerase 1):

Immunohistochemistry of Human esophagus cancer using PARP1 Polyclonal Antibody at dilution of 1:40

Western blot analysis of PARP1 (arrow) using rabbit polyclonal PARP1 Antibody (N-term)(ABIN390353). 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected with the PARP1 gene (Lane 2) (Origene Technologies).

This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery... 更多...

抗体

430 产品

ELISA试剂盒

39 产品

蛋白

27 产品

此分类里的别的抗原：

LIG3 (Ligase III, DNA, ATP-Dependent):	LIG3 抗体	LIG3 ELISA试剂盒	LIG3 蛋白
LIG2 - Ligase II, DNA, ATP-Dependent:
PARP2 - Poly (ADP-Ribose) Polymerase 2:	PARP2 抗体	PARP2 ELISA试剂盒	PARP2 蛋白
LIG1 (Ligase I, DNA, ATP-Dependent):	LIG1 抗体	LIG1 ELISA试剂盒	LIG1 蛋白
ATM (Ataxia Telangiectasia Mutated):	ATM 抗体	ATM ELISA试剂盒	ATM 蛋白
TRIM29 (Tripartite Motif Containing 29):	TRIM29 抗体		TRIM29 蛋白
ATR (Ataxia Telangiectasia and Rad3 Related):	ATR 抗体
RBBP8 - Retinoblastoma Binding Protein 8:	RBBP8 抗体		RBBP8 蛋白
MRE11A - MRE11 Meiotic Recombination 11 Homolog A (S. Cerevisiae):	MRE11A 抗体	MRE11A ELISA试剂盒	MRE11A 蛋白
RAD50 (RAD50 Homolog (S. Cerevisiae)):	RAD50 抗体	RAD50 ELISA试剂盒	RAD50 蛋白
NBN - Nibrin:	NBN 抗体	NBN ELISA试剂盒	NBN 蛋白
RECQL2 - RECQL2 (ARABIDOPSIS RECQ HELICASE L2), 3'-5' DNA Helicase/ ATP-Dependent Helicase/ Four-Way Junction Helicase/ Protein Binding:	RECQL2 抗体

Homologous Recombination

RAD51 (DNA Repair Protein Homolog 1):

Immunohistochemistry of Human breast cancer using RAD51 Polyclonal Antibody at dilution of 1:40

RAD51 MaxPab polyclonal antibody. Western Blot analysis of RAD51 expression in human colon.

The protein encoded by this gene is a member of the RAD51 protein family. RAD51 family members are highly similar to bacterial RecA and Saccharomyces cerevisiae Rad51, and are known to be involved in the homologous recombination and repair of DNA. This protein can interact with the ssDNA-binding protein RPA and RAD52, and it is thought to play roles in homologous pairing and strand transfer of... 更多...

抗体

236 产品

ELISA试剂盒

4 产品

蛋白

13 产品

BRCA1 (Breast Cancer 1):

Immunohistochemistry (IHC) analyzes of BRCA1 pAb in paraffin-embedded human breast carcinoma tissue.

Immunofluorescence analysis of A549 cell using BRCA1 antibody. Blue: DAPI for nuclear staining.

This gene encodes a nuclear phosphoprotein that plays a role in maintaining genomic stability, and it also acts as a tumor suppressor. The encoded...

BRCA1 抗体

BRCA1 ELISA试剂盒

BRCA1 蛋白

BRCA2 (Breast Cancer 2, Early Onset):

Detection limit for recombinant GST tagged BRCA2 is 0.03 ng/ml as a capture antibody.

MCF-7 cells were fixed with paraformaldehyde, permeabilized with 0.25% Triton X-100 in PBS, blocked with 10% normal goat serum in PBS, and stained with BRCA2 (3G7) Monoclonal Antibody at 1:200 in blocking solution. Slides were incubated overnight at 4°C followed by FITC conjugated secondary antibody inclubation and DAPI staining of the nucleus.

Inherited mutations in BRCA1 and this gene, BRCA2, confer increased lifetime risk of developing breast or ovarian cancer. Both BRCA1 and BRCA2 are...

BRCA2 抗体

BRCA2 ELISA试剂盒

BRCA2 蛋白

此分类里的别的抗原：

POLE (Polymerase (DNA Directed), Epsilon, Catalytic Subunit):	POLE 抗体
POLE2 - Polymerase (DNA Directed), epsilon 2 (p59 Subunit):	POLE2 抗体		POLE2 蛋白
POLE3 - Polymerase (DNA Directed), epsilon 3 (p17 Subunit):	POLE3 抗体		POLE3 蛋白
POLE4 (Polymerase (DNA-Directed), epsilon 4 (p12 Subunit)):	POLE4 抗体		POLE4 蛋白
POLD1 - Polymerase (DNA Directed), delta 1, Catalytic Subunit 125kDa:	POLD1 抗体	POLD1 ELISA试剂盒	POLD1 蛋白
POLD2 (Polymerase (DNA Directed), delta 2, Accessory Subunit):	POLD2 抗体		POLD2 蛋白
POLD3 - Polymerase (DNA-Directed), delta 3, Accessory Subunit:	POLD3 抗体		POLD3 蛋白
POLD4 (Polymerase (DNA-Directed), delta 4, Accessory Subunit):	POLD4 抗体		POLD4 蛋白
ATM (Ataxia Telangiectasia Mutated):	ATM 抗体	ATM ELISA试剂盒	ATM 蛋白
TRIM29 (Tripartite Motif Containing 29):	TRIM29 抗体		TRIM29 蛋白
ATR (Ataxia Telangiectasia and Rad3 Related):	ATR 抗体
Rad51B - RAD51 Homolog B (S. Cerevisiae):	Rad51B 抗体		Rad51B 蛋白
RAD51C (RAD51 Homolog C (S. Cerevisiae)):	RAD51C 抗体		RAD51C 蛋白
XRCC2 (X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 2):	XRCC2 抗体	XRCC2 ELISA试剂盒	XRCC2 蛋白
XRCC3 (X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 3):	XRCC3 抗体		XRCC3 蛋白
BRIP1 (BRCA1 Interacting Protein C-terminal Helicase 1):	BRIP1 抗体	BRIP1 ELISA试剂盒
BARD1 (BRCA1 Associated RING Domain 1):	BARD1 抗体	BARD1 ELISA试剂盒	BARD1 蛋白
RBBP8 - Retinoblastoma Binding Protein 8:	RBBP8 抗体		RBBP8 蛋白
FAM175A - Family with Sequence Similarity 175, Member A:	FAM175A 抗体		FAM175A 蛋白
UIMC1 - Ubiquitin Interaction Motif Containing 1:	UIMC1 抗体	UIMC1 ELISA试剂盒	UIMC1 蛋白

MRE11A - MRE11 Meiotic Recombination 11 Homolog A (S. Cerevisiae):	MRE11A 抗体	MRE11A ELISA试剂盒	MRE11A 蛋白
RAD50 (RAD50 Homolog (S. Cerevisiae)):	RAD50 抗体	RAD50 ELISA试剂盒	RAD50 蛋白
NBN - Nibrin:	NBN 抗体	NBN ELISA试剂盒	NBN 蛋白
TP53BP1 (Tumor Protein P53 Binding Protein 1):	TP53BP1 抗体	TP53BP1 ELISA试剂盒	TP53BP1 蛋白
RPA1 - Replication Protein A1, 70kDa:	RPA1 抗体	RPA1 ELISA试剂盒	RPA1 蛋白
RPA2 (Replication Protein A2, 32kDa):	RPA2 抗体	RPA2 ELISA试剂盒	RPA2 蛋白
RPA3 (Replication Protein A3, 14kDa):	RPA3 抗体		RPA3 蛋白
PALB2 (Partner and Localizer of BRCA2):	PALB2 抗体	PALB2 ELISA试剂盒	PALB2 蛋白
TOP3A (Topoisomerase (DNA) III alpha):	TOP3A 抗体	TOP3A ELISA试剂盒
RMI1 (Homolog of Yeast RecQ-mediated Genome Instability 1):	RMI1 抗体	RMI1 ELISA试剂盒	RMI1 蛋白
RMI2 (RMI2, RecQ Mediated Genome Instability 2, Homolog (S. Cerevisiae)):	RMI2 抗体	RMI2 ELISA试剂盒	RMI2 蛋白
DNA2 (DNA Replication Helicase 2 Homolog (Yeast)):	DNA2 抗体
RECQL2 - RECQL2 (ARABIDOPSIS RECQ HELICASE L2), 3'-5' DNA Helicase/ ATP-Dependent Helicase/ Four-Way Junction Helicase/ Protein Binding:	RECQL2 抗体
RAD54L (RAD54-Like (S. Cerevisiae)):	RAD54L 抗体		RAD54L 蛋白
MUS81 (MUS81 Endonuclease Homolog (S. Cerevisiae)):	MUS81 抗体		MUS81 蛋白
SLX1B - SLX1 Structure-Specific Endonuclease Subunit Homolog B (S. Cerevisiae):	SLX1B 抗体		SLX1B 蛋白
BTBD12 - BTB (POZ) Domain Containing 12:	BTBD12 抗体
GEN1 (Gen Endonuclease Homolog 1 (Drosophila)):	GEN1 抗体

Single Strand Annealing

MSH2 - MutS Homolog 2, Colon Cancer, Nonpolyposis Type 1 (E. Coli)

10 × 20 × IHC of MSH-2 (clone FE11) on FFPE tonsil tissue. Citrate pH 6.5, DAB, Hematoxylin

This locus is frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). When cloned, it was discovered to be a human homolog of the E....

MSH2 抗体

MSH2 ELISA试剂盒

MSH2 蛋白

ERCC1 (Excision Repair Cross Complementing Polypeptide-1):

Immunofluorescence analysis analysis of U2OS cell using ERCC1 antibody. Blue: DAPI for nuclear staining.

Detection limit for recombinant GST tagged ERCC1 is approximately 0.03ng/ml as a capture antibody.

The product of this gene functions in the nucleotide excision repair pathway, and is required for the repair of DNA lesions such as those induced...

ERCC1 抗体

ERCC1 ELISA试剂盒

ERCC1 蛋白

此分类里的别的抗原：

MSH3 (MutS Homolog 3 (E. Coli)):	MSH3 抗体
ERCC4 - Excision Repair Cross-Complementing Rodent Repair Deficiency, Complementation Group 4:	ERCC4 抗体	ERCC4 ELISA试剂盒	ERCC4 蛋白
ATM (Ataxia Telangiectasia Mutated):	ATM 抗体	ATM ELISA试剂盒	ATM 蛋白
TRIM29 (Tripartite Motif Containing 29):	TRIM29 抗体		TRIM29 蛋白
ATR (Ataxia Telangiectasia and Rad3 Related):	ATR 抗体
MRE11A - MRE11 Meiotic Recombination 11 Homolog A (S. Cerevisiae):	MRE11A 抗体	MRE11A ELISA试剂盒	MRE11A 蛋白
RAD50 (RAD50 Homolog (S. Cerevisiae)):	RAD50 抗体	RAD50 ELISA试剂盒	RAD50 蛋白
NBN - Nibrin:	NBN 抗体	NBN ELISA试剂盒	NBN 蛋白
RPA1 - Replication Protein A1, 70kDa:	RPA1 抗体	RPA1 ELISA试剂盒	RPA1 蛋白
RPA2 (Replication Protein A2, 32kDa):	RPA2 抗体	RPA2 ELISA试剂盒	RPA2 蛋白
RPA3 (Replication Protein A3, 14kDa):	RPA3 抗体		RPA3 蛋白
RAD52 (RAD52 Homolog (S. Cerevisiae)):	RAD52 抗体	RAD52 ELISA试剂盒	RAD52 蛋白

Break-Induced Replication

MCM2 - Minichromosome Maintenance Complex Component 2

The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of...

MCM2 抗体

MCM2 ELISA试剂盒

MCM2 蛋白

MCM7 - Minichromosome Maintenance Complex Component 7

Immunohistochemistry of Human colon cancer using MCM7 Polyclonal Antibody at dilution of 1:15

MCM7 in Human Mesothelioma Tissue. MCM7 was detected in immersion fixed paraffin-embedded sections of human mesothelioma tissue using Rabbit Anti-Human MCM7 Monoclonal Antibody. Specific staining was localized to nuclei.

The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of...

MCM7 抗体

MCM7 ELISA试剂盒

MCM7 蛋白

此分类里的别的抗原：

RAD51 (DNA Repair Protein Homolog 1):	RAD51 抗体	RAD51 ELISA试剂盒	RAD51 蛋白
MRE11A - MRE11 Meiotic Recombination 11 Homolog A (S. Cerevisiae):	MRE11A 抗体	MRE11A ELISA试剂盒	MRE11A 蛋白
RAD50 (RAD50 Homolog (S. Cerevisiae)):	RAD50 抗体	RAD50 ELISA试剂盒	RAD50 蛋白
NBN - Nibrin:	NBN 抗体	NBN ELISA试剂盒	NBN 蛋白
RPA1 - Replication Protein A1, 70kDa:	RPA1 抗体	RPA1 ELISA试剂盒	RPA1 蛋白
RPA2 (Replication Protein A2, 32kDa):	RPA2 抗体	RPA2 ELISA试剂盒	RPA2 蛋白
RPA3 (Replication Protein A3, 14kDa):	RPA3 抗体		RPA3 蛋白
MCM3 (Minichromosome Maintenance Complex Component 3):	MCM3 抗体	MCM3 ELISA试剂盒	MCM3 蛋白
MCM4 (Minichromosome Maintenance Deficient 4):	MCM4 抗体	MCM4 ELISA试剂盒	MCM4 蛋白
MCM5 - Minichromosome Maintenance Complex Component 5:	MCM5 抗体	MCM5 ELISA试剂盒	MCM5 蛋白
MCM6 - Minichromosome Maintenance Complex Component 6:	MCM6 抗体	MCM6 ELISA试剂盒	MCM6 蛋白

Base Excision Repair

OGG1 (8-Oxoguanine DNA Glycosylase):

OGG1 MaxPab polyclonal antibody. Western Blot analysis of OGG1 expression in human kidney.

OGG1 MaxPab rabbit polyclonal antibody. Western Blot analysis of OGG1 expression in Jurkat.

Formalin-fixed and paraffin embedded human colon cancer labeled with Rabbit Anti-OGG1 Polyclonal Antibody, Unconjugated 1:200 followed by conjugation to the secondary antibody and DAB staining

This gene encodes the enzyme responsible for the excision of 8-oxoguanine, a mutagenic base byproduct which occurs as a result of exposure to reactive oxygen. The action of this enzyme includes lyase activity for chain cleavage. Alternative splicing of the C-terminal region of this gene classifies splice variants into two major groups, type 1 and type 2, depending on the last exon of the... 更多...

抗体

147 产品

ELISA试剂盒

18 产品

蛋白

20 产品

UNG (Uracil-DNA Glycosylase):

Western blot analysis of UNG using anti- UNG antibody . Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each Lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat skeletal muscle tissue lysates, Lane 2: rat heart tissue lysates, Lane 3: mouse skeletal muscle tissue lysates, Lane 4: 22RV1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- UNG antigen affinity purified polyclonal antibody (Catalog # ) at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for UNG at approximately 39KD. The expected band size for UNG is at 35KD.

Western Blot analysis of UNG expression in transfected 293T cell line by UNG MaxPab polyclonal antibody. Lane 1: UNG transfected lysate(33.44 KDa). Lane 2: Non-transfected lysate.

This gene encodes one of several uracil-DNA glycosylases. One important function of uracil-DNA glycosylases is to prevent mutagenesis by...

UNG 抗体

UNG ELISA试剂盒

UNG 蛋白

APEX1 - APEX Nuclease (Multifunctional DNA Repair Enzyme) 1

Western blot analysis of APE1 using anti-APE1 antibody . Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each Lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human COLO-320 whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: Rabbit IgG, Lane 6: Marker 1113, Lane 7: human A549 whole cell lysates , Lane 8: human PANC-1 whole cell lysates, Lane 9: human 22RV1 whole cell lysates, Lane 10: human MDA-MB-453 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-APE1 antigen affinity purified monoclonal antibody (Catalog # ) at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a Biotin Conjugated goat anti-mouse IgG secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system.

APEX1 MaxPab polyclonal antibody. Western Blot analysis of APEX1 expression in human liver.

Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that...

APEX1 抗体

APEX1 ELISA试剂盒

APEX1 蛋白

此分类里的别的抗原：

MPG - N-Methylpurine-DNA Glycosylase:	MPG 抗体	MPG ELISA试剂盒	MPG 蛋白
SMUG1 - Single-Strand-Selective Monofunctional Uracil-DNA Glycosylase 1:	SMUG1 抗体	SMUG1 ELISA试剂盒	SMUG1 蛋白
TDG - Thymine-DNA Glycosylase:	TDG 抗体	TDG ELISA试剂盒	TDG 蛋白
MBD4 (Methyl-CpG Binding Domain Protein 4):	MBD4 抗体		MBD4 蛋白
MUTYH - MutY Homolog (E. Coli):	MUTYH 抗体	MUTYH ELISA试剂盒	MUTYH 蛋白
NEIL1 (Endonuclease VIII-Like 1):	NEIL1 抗体	NEIL1 ELISA试剂盒	NEIL1 蛋白
NEIL2 (Nei Endonuclease VIII-Like 2 (E. Coli)):	NEIL2 抗体		NEIL2 蛋白
NEIL3 (Nei Endonuclease VIII-Like 3 (E. Coli)):	NEIL3 抗体	NEIL3 ELISA试剂盒	NEIL3 蛋白
POLB (Polymerase (DNA Directed), beta):	POLB 抗体	POLB ELISA试剂盒	POLB 蛋白
POLD1 - Polymerase (DNA Directed), delta 1, Catalytic Subunit 125kDa:	POLD1 抗体	POLD1 ELISA试剂盒	POLD1 蛋白
POLD2 (Polymerase (DNA Directed), delta 2, Accessory Subunit):	POLD2 抗体		POLD2 蛋白
POLD3 - Polymerase (DNA-Directed), delta 3, Accessory Subunit:	POLD3 抗体		POLD3 蛋白
POLD4 (Polymerase (DNA-Directed), delta 4, Accessory Subunit):	POLD4 抗体		POLD4 蛋白
FEN1 (Flap Structure-Specific Endonuclease 1):	FEN1 抗体	FEN1 ELISA试剂盒	FEN1 蛋白
LIG1 (Ligase I, DNA, ATP-Dependent):	LIG1 抗体	LIG1 ELISA试剂盒	LIG1 蛋白

Nuleotide Excision Repair

POLR2B - Polymerase (RNA) II (DNA Directed) Polypeptide B, 140kDa

ICC/IF Image Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using RPB2, antibody at 1:200 dilution.

This gene encodes the second largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit, in combination with at least two other polymerase subunits, forms a structure within the polymerase that maintains contact in the active site of the enzyme between the DNA template and the newly synthesized RNA. [provided by RefSeq, Jul 2008]. 更多...

抗体

53 产品

XPC (Xeroderma Pigmentosum, Complementation Group C):

Western Blot at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: MCF-7 whole cell lysate Lane 3: ZR-75-1 whole cell lysate Lysates/proteins at 20 ug per lane.

ICC/IF Image Immunofluorescence analysis of paraformaldehyde-fixed A431, using XPC, antibody at 1:500 dilution.

This gene encodes a component of the nucleotide excision repair (NER) pathway. There are multiple components involved in the NER pathway, including...

XPC 抗体

XPC ELISA试剂盒

XPC 蛋白

DDB1 (Damage Specific DNA Binding Protein 1):

Immunocytochemistry/Immunofluorescence: DDB1 Antibody - Staining of human cell line A549 shows localization to nucleoplasm.

Western blot analysis of MCF-7, Hela and HepG2 cell, using DDB1 Polyclonal Antibody at dilution of 1:350

Antigen: 0.2 µg/100 µL Primary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; Secondary: HRP conjugated Goat-Anti-Rabbit IgG at 1: 5000; TMB staining; Read the data in MicroplateReader by 450

The protein encoded by this gene is the large subunit (p127) of the heterodimeric DNA damage-binding (DDB) complex while another protein (p48)...

DDB1 抗体

DDB1 ELISA试剂盒

DDB1 蛋白

此分类里的别的抗原：

XPA (Xeroderma Pigmentosum, Complementation Group A):	XPA 抗体	XPA ELISA试剂盒	XPA 蛋白
RAD23A (RAD23 Homolog A (S. Cerevisiae)):	RAD23A 抗体		RAD23A 蛋白
RAD23B - RAD23 Homolog B (S. Cerevisiae):	RAD23B 抗体	RAD23B ELISA试剂盒	RAD23B 蛋白
CETN2 (Centrin, EF-Hand Protein, 2):	CETN2 抗体		CETN2 蛋白
DDB2 (Damage-Specific DNA Binding Protein 2, 48kDa):	DDB2 抗体	DDB2 ELISA试剂盒	DDB2 蛋白
ERCC1 (Excision Repair Cross Complementing Polypeptide-1):	ERCC1 抗体	ERCC1 ELISA试剂盒	ERCC1 蛋白
ERCC2 (Excision Repair Cross-Complementing Rodent Repair Deficiency, Complementation Group 2):	ERCC2 抗体		ERCC2 蛋白
ERCC3 - DNA Repair Protein Complementing XP-B Cells:	ERCC3 抗体		ERCC3 蛋白
GTF2H1 - General Transcription Factor IIH, Polypeptide 1, 62kDa:	GTF2H1 抗体	GTF2H1 ELISA试剂盒	GTF2H1 蛋白
GTF2H4 (General Transcription Factor IIH, Polypeptide 4, 52kDa):	GTF2H4 抗体		GTF2H4 蛋白
GTF2H3 (General Transcription Factor IIH, Polypeptide 3, 34kD):	GTF2H3 抗体		GTF2H3 蛋白
GTF2H5 - General Transcription Factor IIH, Polypeptide 5:	GTF2H5 抗体		GTF2H5 蛋白
CDK7 (Cyclin-Dependent Kinase 7):	CDK7 抗体	CDK7 ELISA试剂盒	CDK7 蛋白
RFC1 - Replication Factor C (Activator 1) 1, 145kDa:	RFC1 抗体	RFC1 ELISA试剂盒	RFC1 蛋白
RFC2 (Replication Factor C (Activator 1) 2, 40kDa):	RFC2 抗体		RFC2 蛋白
RFC3 - Replication Factor C (Activator 1) 3, 38kDa:	RFC3 抗体		RFC3 蛋白
RFC4 (Replication Factor C (Activator 1) 4, 37kDa):	RFC4 抗体		RFC4 蛋白
RFC5 (Replication Factor C (Activator 1) 5, 36.5kDa):	RFC5 抗体		RFC5 蛋白
POLK - Polymerase (DNA Directed) kappa:	POLK 抗体		POLK 蛋白
ERCC4 - Excision Repair Cross-Complementing Rodent Repair Deficiency, Complementation Group 4:	ERCC4 抗体	ERCC4 ELISA试剂盒	ERCC4 蛋白

ERCC5 - DNA Repair Protein Complementing XP-G Cells:	ERCC5 抗体	ERCC5 ELISA试剂盒	ERCC5 蛋白
ERCC6 (Excision Repair Cross-Complementing Rodent Repair Deficiency, Complementation Group 6):	ERCC6 抗体		ERCC6 蛋白
ERCC8 (Excision Repair Cross-Complementing Rodent Repair Deficiency, Complementation Group 8):	ERCC8 抗体		ERCC8 蛋白
POLE (Polymerase (DNA Directed), Epsilon, Catalytic Subunit):	POLE 抗体
POLE2 - Polymerase (DNA Directed), epsilon 2 (p59 Subunit):	POLE2 抗体		POLE2 蛋白
POLE3 - Polymerase (DNA Directed), epsilon 3 (p17 Subunit):	POLE3 抗体		POLE3 蛋白
POLE4 (Polymerase (DNA-Directed), epsilon 4 (p12 Subunit)):	POLE4 抗体		POLE4 蛋白
PCNA (Proliferating Cell Nuclear Antigen):	PCNA 抗体	PCNA ELISA试剂盒	PCNA 蛋白
XRCC1 (X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 1):	XRCC1 抗体	XRCC1 ELISA试剂盒	XRCC1 蛋白
FEN1 (Flap Structure-Specific Endonuclease 1):	FEN1 抗体	FEN1 ELISA试剂盒	FEN1 蛋白
LIG1 (Ligase I, DNA, ATP-Dependent):	LIG1 抗体	LIG1 ELISA试剂盒	LIG1 蛋白
RPA1 - Replication Protein A1, 70kDa:	RPA1 抗体	RPA1 ELISA试剂盒	RPA1 蛋白
RPA2 (Replication Protein A2, 32kDa):	RPA2 抗体	RPA2 ELISA试剂盒	RPA2 蛋白
RPA3 (Replication Protein A3, 14kDa):	RPA3 抗体		RPA3 蛋白

Mismatch Repair

MSH2 - MutS Homolog 2, Colon Cancer, Nonpolyposis Type 1 (E. Coli)

This locus is frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). When cloned, it was discovered to be a human homolog of the E. coli mismatch repair gene mutS, consistent with the characteristic alterations in microsatellite sequences (RER+ phenotype) found in HNPCC. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012]. 更多...

抗体

3 产品

ELISA试剂盒

2 产品

蛋白

2 产品

MSH3 (MutS Homolog 3 (E. Coli)):

Immunohistochemistry of Human colon cancer using MSH3 Polyclonal Antibody at dilution of 1:20

Western blot analysis of MSH3 on a HeLa cell lysate (Human cervical epitheloid carcinoma, ATCC CCL-2). 2 µg/mL (lane 1), 1 µg/mL (lane 2) and 0.5 µg/mL (lane 3) of the mouse anti-human MSH3 antibody were used.

Western Blot: MSH3 Antibody [NBP2-19418] - Sample (30 ug of whole cell lysate) A: HCT116 5% SDS PAGE gel, diluted at 1:1000.

The protein encoded by this gene forms a heterodimer with MSH2 to form MutS beta, part of the post-replicative DNA mismatch repair system. MutS...

MSH3 抗体

MSH6 (MutS Homolog 6 (E. Coli)):

Immunofluorescence analysis of A549 cell using MSH6 antibody. Blue: DAPI for nuclear staining.

This gene encodes a protein similar to the MutS protein. In E. coli, the MutS protein helps in the recognition of mismatched nucleotides, prior to...

MSH6 抗体

MSH6 蛋白

此分类里的别的抗原：

MLH1 (MutL Homolog 1, Colon Cancer, Nonpolyposis Type 2 (E. Coli)):	MLH1 抗体	MLH1 ELISA试剂盒	MLH1 蛋白
PMS2 (PMS2 Postmeiotic Segregation Increased 2 (S. Cerevisiae)):	PMS2 抗体		PMS2 蛋白
RFC1 - Replication Factor C (Activator 1) 1, 145kDa:	RFC1 抗体	RFC1 ELISA试剂盒	RFC1 蛋白
RFC2 (Replication Factor C (Activator 1) 2, 40kDa):	RFC2 抗体		RFC2 蛋白
RFC3 - Replication Factor C (Activator 1) 3, 38kDa:	RFC3 抗体		RFC3 蛋白
RFC4 (Replication Factor C (Activator 1) 4, 37kDa):	RFC4 抗体		RFC4 蛋白
RFC5 (Replication Factor C (Activator 1) 5, 36.5kDa):	RFC5 抗体		RFC5 蛋白
POLD1 - Polymerase (DNA Directed), delta 1, Catalytic Subunit 125kDa:	POLD1 抗体	POLD1 ELISA试剂盒	POLD1 蛋白
POLD2 (Polymerase (DNA Directed), delta 2, Accessory Subunit):	POLD2 抗体		POLD2 蛋白
POLD3 - Polymerase (DNA-Directed), delta 3, Accessory Subunit:	POLD3 抗体		POLD3 蛋白
PCNA (Proliferating Cell Nuclear Antigen):	PCNA 抗体	PCNA ELISA试剂盒	PCNA 蛋白
LIG1 (Ligase I, DNA, ATP-Dependent):	LIG1 抗体	LIG1 ELISA试剂盒	LIG1 蛋白
RPA1 - Replication Protein A1, 70kDa:	RPA1 抗体	RPA1 ELISA试剂盒	RPA1 蛋白
RPA2 (Replication Protein A2, 32kDa):	RPA2 抗体	RPA2 ELISA试剂盒	RPA2 蛋白
RPA3 (Replication Protein A3, 14kDa):	RPA3 抗体		RPA3 蛋白
EXO1 - Exonuclease 1:	EXO1 抗体	EXO1 ELISA试剂盒	EXO1 蛋白

Direct Reversal

MGMT (O6-Methylguanine-DNA-Methyltransferase):

Western blot analysis of MGMT antibody (C-term) (ABIN391514) in CEM cell line lysates (35 µg/lane). MGMT (arrow) was detected using the purified polyclonal antibody.

Immunoperoxidase of monoclonal antibody to MGMT on formalin-fixed paraffin-embedded human tonsil (antibody concentration 3 µg/mL).

Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a 更多...

抗体

219 产品

ELISA试剂盒

34 产品

蛋白

19 产品

ALKBH - AlkB, Alkylation Repair Homolog 1 (E. Coli)

Immunofluorescence of purified MaxPab antibody to ALKBH on HeLa cell. [antibody concentration 10 ug/ml]

Western blot analysis of Mouse pancreas tissue, using ALKBH1 Polyclonal Antibody at dilution of 1:250

This gene encodes a homolog to the E. coli alkB gene product. The E. coli alkB protein is part of the adaptive response mechanism of DNA alkylation...

ALKBH 抗体

ALKBH 蛋白

ALKBH2 - AlkB, Alkylation Repair Homolog 2

Western blot analysis of Human fetal liver and seminoma tissue, RAW264.7 cell, using ALKBH2 Polyclonal Antibody at dilution of 1:650

Immunocytochemistry/Immunofluorescence: ABH2 Antibody - Staining of human cell line HEK 293 shows localization to nucleoplasm.

The Escherichia coli AlkB protein protects against the cytotoxicity of methylating agents by repair of the specific DNA lesions generated in...

ALKBH2 抗体

ALKBH2 蛋白

此分类里的别的抗原：

ALKBH3 - AlkB, Alkylation Repair Homolog 3 (E. Coli):	ALKBH3 抗体	ALKBH3 ELISA试剂盒	ALKBH3 蛋白

Trans-Lesion Synthesis

POLI - Polymerase (DNA Directed) iota

Western Blot analysis of POLI expression in transfected 293T cell line by POLI monoclonal antibody (M01), clone 8G9. Lane 1: POLI transfected lysate(80.3 KDa). Lane 2: Non-transfected lysate. POLI monoclonal antibody (M01), clone 8G9 Western Blot analysis of POLI expression in HeLa NE.

POLI monoclonal antibody (M01), clone 8G9 Western Blot analysis of POLI expression in HeLa NE

Western blot analysis of DNA Polymerase iota using anti-DNA Polymerase iota antibody . Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each Lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: human A549 whole cell lysates, Lane 4: human SK-OV-3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-DNA Polymerase iota antigen affinity purified polyclonal antibody (Catalog # ) at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for DNA Polymerase iota at approximately 83KD. The expected band size for DNA Polymerase iota is at 83KD.

Error-prone DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity...

POLI 抗体

POLI 蛋白

POLH - Polymerase (DNA Directed), eta

Western blot analysis of A431 cell, using POLH Polyclonal Antibody at dilution of 1:500

Immunofluorescence analysis analysis of U2OS cell using POLH antibody. Blue: DAPI for nuclear staining.

Immunoprecipitation of POLH transfected lysate using anti-POLH MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead , and immunoblotted with POLH purified MaxPab mouse polyclonal antibody (B01P) .

This gene encodes a member of the Y family of specialized DNA polymerases. It copies undamaged DNA with a lower fidelity than other DNA-directed...

POLH 抗体

POLH 蛋白

此分类里的别的抗原：

REV3L (REV3-Like, Polymerase (DNA Directed), Zeta, Catalytic Subunit):	REV3L 抗体
REV1 (REV1, Polymerase (DNA Directed)):	REV1 抗体		REV1 蛋白
POLK - Polymerase (DNA Directed) kappa:	POLK 抗体		POLK 蛋白
PCNA (Proliferating Cell Nuclear Antigen):	PCNA 抗体	PCNA ELISA试剂盒	PCNA 蛋白

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