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TrueBlot® Antibodies, Kits, Tools

TrueBlot® is a monoclonal secondary antibody by Rockland Immunochemicals, Inc. that detects only the fully native, non-denatured form of immunoglobulins. Consequently, it detects only the primary antibody used to detect the protein of interest in the Western blot and not the additional heavy and light chains of the IP antibody that reaches the gel as part of the immunoprecipitation contaminants. TrueBlot® technology is used in a wide variety of products: antibodies, beads, kits and other tools are available.

TrueBlot® monoclonal Secondary Antibodies

Take advantage of the unique properties of TrueBlot® monoclonal secondary antibodies to achieve accurate, reproducible, sensitive, and low-background detection of target proteins in Western blots, especially when you need to generate publication-quality Westerns or detect immunoprecipitated (IP) proteins! TrueBlot secondary antibodies are highly specific. They detect only native, unreduced IgG, optimize detection of the primary antibody, and reduce background signal from antibodies used prior to SDS-PAGE, such as during IP.

Benefits of TrueBlot® Secondary Antibodies

TrueBlot® monoclonal secondary antibodies detect the native, non-reduced form of IgG over the reduced form of IgG in a variety of species. For Western blot experiments of immunoprecipitated proteins (IP–Western blot) TrueBlot® secondary antibodies massively reduce the interference caused by the heavy and light chain of the immunoprecipitating antibody. TrueBlot® secondary antibodies therefore allow you to detect your protein of interest without heavy/light chain interference.

  • Only detect your target protein
  • Multipe Conjugates available
  • Suitable for non-IP Western blot experiments as well
  • Ideal for studying post-translational protein modifications (e.g., phosphorylation, acetylation) and protein-protein interactions
Product
Conjugate
Application
Cat. No.
Validations
Quantity
Price
Datasheet
Conjugate HRP
Application IP, WB
Cat. No. ABIN1589972
Validations
  • (6)
  • collections(2)
Quantity 200 μL
Price $229.00
Conjugate HRP
Application IP, WB
Cat. No. ABIN1589974
Validations
  • (89)
  • collections(4)
Quantity 200 μL
Price $229.00
Conjugate HRP
Application IP, WB
Cat. No. ABIN1589977
Validations
  • (73)
  • collections(3)
Quantity 200 μL
Price $229.00
Conjugate Biotin
Application IP, WB
Cat. No. ABIN1589968
Validations
  • (6)
  • collections(1)
Quantity 100 μg
Price $201.00
Conjugate HRP
Application IP, WB
Cat. No. ABIN1589970
Validations
  • (10)
  • collections(2)
Quantity 200 μL
Price $229.00
Conjugate FITC
Application FLISA, FACS, FM, IHC, WB
Cat. No. ABIN6698841
Validations
  • collections(4)
Quantity 100 μL
Price $226.00
Conjugate DyLight 800
Application FLISA, FACS, FM, IHC, WB
Cat. No. ABIN6698840
Validations
  • (3)
  • collections(2)
Quantity 100 μL
Price $226.00
Conjugate DyLight 680
Application FLISA, FACS, FM, IHC, WB
Cat. No. ABIN6698839
Validations
  • (2)
  • collections(2)
Quantity 100 μL
Price $226.00
Conjugate FITC
Application FLISA, FACS, FM, IHC, WB
Cat. No. ABIN6698838
Validations
  • collections(3)
Quantity 100 μL
Price $226.00
Conjugate DyLight 800
Application FLISA, FACS, FM, IHC, WB
Cat. No. ABIN6698837
Validations
  • (3)
  • collections(2)
Quantity 100 μL
Price $226.00
Conjugate DyLight 680
Application FLISA, FACS, FM, IHC, WB
Cat. No. ABIN6698836
Validations
  • (1)
  • collections(4)
Quantity 100 μL
Price $226.00

TrueBlot® Western Blot Kits

TrueBlot® Western blot kits contain all critical reagents, buffer solutions, substrates for Western blotting and immunoprecipitation of samples. The TrueBlot® Second Step immunoblotting reagents are used in combination with the user's own IP antibody or WB antibody.

Product
Cat. No.
Validations
Quantity
Price
Datasheet
Cat. No. ABIN1589978
Validations
  • (5)
  • collections(2)
Quantity 1 set
Price $314.00
Cat. No. ABIN1589981
Validations
  • (5)
  • collections(2)
Quantity 1 kit
Price $303.00
Cat. No. ABIN1589980
Validations
  • (5)
  • collections(2)
Quantity 1 set
Price $293.00
Cat. No. ABIN1589983
Validations
  • (5)
  • collections(2)
Quantity 1 kit
Price $293.00
Cat. No. ABIN1589979
Validations
  • (5)
  • collections(2)
Quantity 1 set
Price $251.00
Cat. No. ABIN1589982
Validations
  • (5)
  • collections(2)
Quantity 1 kit
Price $307.00
Cat. No. ABIN6809838
Validations
  • collections(2)
Quantity 1 kit
Price $504.90
Cat. No. ABIN6809839
Validations
  • collections(3)
Quantity 1 kit
Price $504.90
Cat. No. ABIN6809837
Validations
  • collections(3)
Quantity 1 kit
Price $504.90
Cat. No. ABIN6720435
Validations
  • collections(1)
Quantity 1 kit
Price $399.00
Cat. No. ABIN6720436
Validations
  • collections(1)
Quantity 1 kit
Price $399.00

TrueBlot® IP Beads and Magnetic Beads

The TrueBlot® Immunoprecipitation beads are specifically designed, tested, and quality-controlled to provide optimal results in the capture and purification of targets from cell lysates and serums. They show excellent binding capacities. They are stable, pre-blocked beads for cleaner purifications. Species-specific Ig IP beads are available in an agarose or magnetic bead format. Various magnetic beads for general use, conjugated with protein A, protein G, streptavidin or biotin are available.

Product
Cat. No.
Quantity
Price
Datasheet
Cat. No. ABIN1589966
Quantity 2.5 mL
Price $221.00
Cat. No. ABIN6809835
Quantity 2 mL
Price $275.00
Cat. No. ABIN1589965
Quantity 2.5 mL
Price $197.00
Cat. No. ABIN6809833
Quantity 2 mL
Price $262.90
Cat. No. ABIN1589964
Quantity 2.5 mL
Price $197.00
Cat. No. ABIN6809831
Quantity 2 mL
Price $267.30
Cat. No. ABIN6809840
Quantity 2 mL
Price $276.10
Cat. No. ABIN6809842
Quantity 2 mL
Price $256.30
Cat. No. ABIN6809844
Quantity 2 mL
Price $256.30
Cat. No. ABIN6809846
Quantity 2 mL
Price $283.80

TrueBlot® Citations (Highlights)

Journal of Virology:

Nature:

  • Anti-Mouse Ig HRP ULTRA (TrueBlot®) used in: Bonono et al (2017): BAP1 regulates IP3R3-mediated Ca2+ flux to mitochondria suppressing cell transformation. Nature 546 (7659) 549-553.
  • Anti-Rabbit IgG HRP (TrueBlot®) used in: Emmett et al. (2017): Histone deacetylase 3 prepares brown adipose tissue for acute thermogenic challenge. Nature 546 (7659) 544-548.
  • Anti-Rabbit IgG HRP (TrueBlot®) used in: Perraki et al. (2018): Phosphocode-dependent functional dichotomy of a common co-receptor in plant signalling. Nature 561 (7722) 248-252.

Protocols

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