+1 877 302 8632
+1 888 205 9894 (Toll-free)

RFP 抗体

RFP 适用: Discosoma WB, ELISA, IF, IP, IHC, FACS, IHC (fro), IHC (p) 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN129578
加上运输费 $50.00
发货至: 美国
  • Key Features
    • Frequently cited in scientific publications with 300+ citations
    • Validated inhouse for relevant applications
    • Extensive validation report for IF provided by one of your peers
    • Designed to detect RFP and its variants
    抗原 See all RFP 抗体
    RFP (Red Fluorescent Protein (RFP))
    • 48
    • 1
    • 21
    • 11
    • 6
    • 5
    • 3
    • 3
    • 27
    • 22
    • 15
    • 5
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This RFP antibody is un-conjugated
    • 23
    • 21
    • 14
    • 10
    • 9
    • 7
    • 5
    • 4
    • 2
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Polyclonal RFP antibody is designed to detect RFP and its variants.
    交叉反应 (详细)
    Expect reactivity against RFP and its variants: mCherry, tdTomato, mBanana, mPlum, mOrange and mStrawberry. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum and purified and partially purified Red Fluorescent Protein (Discosoma).
    No reaction was observed against Human, Mouse or Rat serum proteins.

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Red Fluorescent Protein (Discosoma) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

    Preadsorption: Pre-adsorbed

    Sterile filtered
    The immunogen is a Red Fluorescent Protein (RFP) fusion protein corresponding to the full length amino acid sequence (234aa) derived from the mushroom polyp coral Discosoma.
    Immunogen Type: Recombinant Protein
  • 应用备注
    Suggested dilutions:
    ELISA: 1:28,700 - 1:48,700
    IF: 1:200 - 1:2,000
    FACS: 1:200 - 1:2,000
    WB: 1:1,000 - 1:5,000
    IHC: 1:200 - 1:2,000
    IP: User Optimized

    This antibody can be used to detect RFP by ELISA (sandwich or capture) for the direct binding of antigen. Biotin conjugated polyclonal anti-RFP used in a sandwich ELISA with unconjugated anti-RFP is well suited to titrate RFP in solution. The detection antibody conjugated to biotin is subsequently reacted with streptavidin conjugated HRP.
    Fluorochrome conjugated polyclonal anti-RFP can be used to detect RFP by immunofluorescence microscopy in cell expression systems and can detect RFP containing inserts. Significant amplification of signal is achieved using fluorochrome conjugated polyclonal anti-RFP relative to the fluorescence of RFP alone.
    For immunoblotting use either alkaline phosphatase or peroxidase conjugated polyclonal anti-RFP to detect RFP or RFP containing proteins on western blots.
    Optimal titers for applications should be determined by the researcher.

  • 生效 #101103 (Immunofluorescence)
    Centre d'Immunologie de Marseille-Luminy
    Lot Number
    Method validated
    Positive Control
    CX3CL1mcherry+ murine thymus
    Negative Control
    CX3CL1 WT murine thymus

    Passed. The RFP antibody ABIN129578 specifically labels RFP+ cells in CX3CL1mcherry+ murine thymus in immunofluorescence, consistent with the expression pattern of CX3CL1mcherry.

    Validation Images
    Full Methods
    Primary Antibody
    Secondary Antibody
    donkey Fab'2 anti-rabbit A647 conjugated antibody (Jackson Immunoresearch, 711-606-152, lot 128806)
    Full Protocol
    • Harvest thymus from 7 weeks old mice in 1X Dulbecco's phosphate buffered saline (DPBS) (Gibco Life Technologies, 14200-067).
    • Fix mouse thymus in antigen fix (Diapath, P0014) for 2h at 4?C.
    • Wash tissue in 0.1M pH7.4 phosphate buffer at for 1h at 4?C.
    • Dehydrate tissue in 30% sucrose solution at 4?C ON.
    • Snap freeze tissue in Tissue Freezing Medium (ElectroMicroscopy Science, 72592-C) at -80?C.
    • Cut blocks into 25?m sections using a cryostat (Leica, CM3050 S).
    • Transfer sections to a slide.
    • Create a hydrophobic barrier on the slide around sections with Dako pen (Dako, S2002, lot 00081640).
    • Place slide in a humidified chamber and rehydrate sections in 0.1 M TrisHCl pH7.4 for 10min at RT.
    • Gently remove buffer by tapping slide.
    • Permeabilize tissue in 0.1M TrisHCl pH7.4 containing 2% Triton X-100 (Sigma, lot 015K0039) and 0.5% BSA, for 20min at RT.
    • Incubate sections with primary RFP antibody (Red Fluorescent Protein) (AA 234) (antibodies-online, ABIN129578, lot 34945) diluted 1:1000 0.1M TrisHCl pH7.4 containing 2% Triton X-100 (Sigma, batch 015K0039) and 0.5% BSA for 2h at RT.
    • Incubate a no primary antibody negative control in parallel in 0.1M TrisHCl pH7.4 containing 2% Triton X-100 (Sigma, batch 015K0039) and 0.5% BSA.
    • Wash slides 1x 5min in 0.1M TrisHCl pH7.4.
    • Incubate sections with secondary donkey Fab'2 anti-rabbit AF647 conjugated antibody (Jackson Immunoresearch, 711-606-152, lot 128806) diluted 1:300 in 0.1M TrisHCl pH7.4 containing 2% Triton X-100 (Sigma, batch 015K0039) and 0.5% BSA for 2h at RT.
    • Wash slides 1x 5min in 0.1M TrisHCl pH7.4.
    • Add approximately 10µL of Slowfade Gold antifade reagent (ThermoFisher Scientific, S36937, lot 1226836) for each section and mount cover slip.
    • Image acquisition on an LSM 880 (Zeiss), 20x magnification, 1000 resolution.
    Experimental Notes
  • 状态
    1.0 mg/mL
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide
    Sodium azide
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Avoid cycles of freezing and thawing.
    4 °C/-20 °C
    Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
    12 months
  • Simpson Ragdale, Clements, Tang, Deltcheva, Andreassi, Lai, Chang, Pandrea, Andrew, Game, Uddin, Ellis, Enver, Riccio, Marguerat, Parrinello: "Injury primes mutation-bearing astrocytes for dedifferentiation in later life." in: Current biology : CB, (2023) (PubMed).

    Amodeo, Davies, Martinez-Segura, Clements, Ragdale, Bailey, Dos Santos, MacRae, Mokochinski, Kramer, Garcia-Diaz, Gould, Marguerat, Parrinello: "Diet suppresses glioblastoma initiation in mice by maintaining quiescence of mutation-bearing neural stem cells." in: Developmental cell, (2023) (PubMed).

    Middelkamp, Ruck, Krisp, Sumisławski, Mohammadi, Dottermusch, Meister, Küster, Schlüter, Windhorst, Neumann: "Overexpression of Lin28A in neural progenitor cells in vivo does not lead to brain tumor formation but results in reduced spine density." in: Acta neuropathologica communications, Vol. 9, Issue 1, pp. 185, (2022) (PubMed).

    Pouchelon, Vergara, McMahon, Gorissen, Lin, Vormstein-Schneider, Niehaus, Burbridge, Wester, Sherer, Fernandez-Otero, Allaway, Pelkey, Chittajallu, McBain, Fan, Nasse, Wildenberg, Fishell et al.: "A versatile viral toolkit for functional discovery in the nervous system. ..." in: Cell reports methods, Vol. 2, Issue 6, pp. 100225, (2022) (PubMed).

    Dumoulin, Zuñiga, Stoeckli: "Axon guidance at the spinal cord midline-A live imaging perspective." in: The Journal of comparative neurology, (2021) (PubMed).

    Park, Lofton, Li, Rasin: "Extrinsic Regulators of mRNA Translation in Developing Brain: Story of WNTs." in: Cells, Vol. 10, Issue 2, (2021) (PubMed).

    Dottermusch, Sumisławski, Krevet, Middelkamp, Voß, Siebels, Bartsch, Sotlar, Meyer, Frank, Korshunov, Glatzel, Schüller, Neumann: "Co-activation of Sonic hedgehog and Wnt signaling in murine retinal precursor cells drives ocular lesions with features of intraocular medulloepithelioma." in: Oncogenesis, Vol. 10, Issue 11, pp. 78, (2021) (PubMed).

    Brooks, Clements, Burden, Kocher, Richards, Devesa, Zakka, Woodberry, Ellis, Jaunmuktane, Brandner, Morrison, Pollard, Dirks, Marguerat, Parrinello: "The white matter is a pro-differentiative niche for glioblastoma." in: Nature communications, Vol. 12, Issue 1, pp. 2184, (2021) (PubMed).

    Dannhäuser, Lux, Hu, Selcho, Chen, Ehmann, Sachidanandan, Stopp, Pauls, Pawlak, Langenhan, Soba, Rittner, Kittel: "Antinociceptive modulation by the adhesion GPCR CIRL promotes mechanosensory signal discrimination." in: eLife, Vol. 9, (2021) (PubMed).

    Bellomo, Mondor, Spinelli, Lagueyrie, Stewart, Brouilly, Malissen, Clatworthy, Bajénoff: "Reticular Fibroblasts Expressing the Transcription Factor WT1 Define a Stromal Niche that Maintains and Replenishes Splenic Red Pulp Macrophages." in: Immunity, Vol. 53, Issue 1, pp. 127-142.e7, (2020) (PubMed).

    Eun, Hong, Jeong, Park, Hwang, Jeong, Choi, Olsson, Hwang, Hyun, Kim: "Transcriptional activities of human elongation factor-1α and cytomegalovirus promoter in transgenic dogs generated by somatic cell nuclear transfer." in: PLoS ONE, Vol. 15, Issue 6, pp. e0233784, (2020) (PubMed).

    Fu, Wang, Chouairi, Rose, Abetov, Miller, Yamulla, Schimenti, Flesken-Nikitin, Nikitin: "Gastric squamous-columnar junction contains a large pool of cancer-prone immature osteopontin responsive Lgr5-CD44+ cells." in: Nature communications, Vol. 11, Issue 1, pp. 84, (2020) (PubMed).

    Schaub, Rose, Frasch: "Yorkie and JNK revert syncytial muscles into myoblasts during Org-1-dependent lineage reprogramming." in: The Journal of cell biology, Vol. 218, Issue 11, pp. 3572-3582, (2020) (PubMed).

    Green, Cavey, Médina Caturegli, Aigouy, Gompel, Prudhomme: "Evolution of Ovipositor Length in Drosophila suzukii Is Driven by Enhanced Cell Size Expansion and Anisotropic Tissue Reorganization." in: Current biology : CB, Vol. 29, Issue 12, pp. 2075-2082.e6, (2020) (PubMed).

    Henschke, Pakan: "Disynaptic cerebrocerebellar pathways originating from multiple functionally distinct cortical areas." in: eLife, Vol. 9, (2020) (PubMed).

    Zhou, Zhong, Peng, Liu, Ding, Sun, Ma, Liu, Chen, Wu, Wang: "Cellular and molecular properties of neural progenitors in the developing mammalian hypothalamus." in: Nature communications, Vol. 11, Issue 1, pp. 4063, (2020) (PubMed).

    Klouda, Condon, Hao, Tian, Lvova, Chakraborty, Nicolls, Zhou, Raby, Yuan: "From 2D to 3D: Promising Advances in Imaging Lung Structure." in: Frontiers in medicine, Vol. 7, pp. 343, (2020) (PubMed).

    Ricci, Tycksen, Celik, Belle, Fontana, Civitelli, Faccio: "Osterix-Cre marks distinct subsets of CD45- and CD45+ stromal populations in extra-skeletal tumors with pro-tumorigenic characteristics." in: eLife, Vol. 9, (2020) (PubMed).

    Kennedy, Rinker, Broadie: "Genetic background mutations drive neural circuit hyperconnectivity in a fragile X syndrome model." in: BMC biology, Vol. 18, Issue 1, pp. 94, (2020) (PubMed).

    Bruens, Ellenbroek, Suijkerbuijk, Azkanaz, Hale, Toonen, Flanagan, Sansom, Snippert, van Rheenen: "Calorie Restriction Increases the Number of Competing Stem Cells and Decreases Mutation Retention in the Intestine." in: Cell reports, Vol. 32, Issue 3, pp. 107937, (2020) (PubMed).

  • 抗原
    RFP (Red Fluorescent Protein (RFP))
    RFP (RFP 产品)
    Synonyms: DsRed, rDsRed, Discosoma sp. Red Fluorescent Protein, Red fluorescent protein drFP583
    Fluorescent proteins such as Discosoma Red Fluorescent Protein (DsRed) from sea anemone Discosoma sp. mushroom or green fluorescent protein (GFP) from Aequorea victoria jellyfish are widely used in research practice. Fusion RFP and GFP commonly serve as marker for gene expression and protein localization. As DsRed and GFP share only 19% identity, therefore, in general, anti-GFP antibodies do not recognize DsRed protein and vice versa. Structurally, Discosoma red fluorescent protein is similar to Aequorea green fluorescent protein in terms of its overall fold (a β-can) and chromophore-formation chemistry. However, Discosoma red fluorescent protein undergoes an additional step in the chromophore maturation and obligates tetrameric structure.
You are here: