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FcRn 抗体

Name: FcRn 抗体
Brand: antibodies-online
SKU: ABIN1774762
Availability: OutOfStock

FcRn 适用: 人 ELISA, IF, FACS, cELISA, Func 宿主: 小鼠 Monoclonal ADM31 unconjugated

Immunofluorescence: huSSECTM cell line overexpressing hFcRn-EGFP using clone ADM31 at 15µg/ml and Texas Red™ conjugated anti-mouse secondary. (FcRn 抗体)

Antibody- or peptide-mediated disruption of the human FcRn–albumin interactions decreases chemical hepatotoxicity. (FcRn 抗体)

Inhibition

293hFcRn-GFP cells were incubated with a range of concentrations of DVN1, ADM11, DVN21, DVN22, DVN23, DVN24, ADM31 or ADM32 at pH 6, then stained for functional binding with (A) 20 μg/mL hIgGAF647, or (B) 200 μg/mL HSAbiotin followed by streptavidin-PE. (FcRn 抗体)

FACS

ELISA

Identification of monoclonal Abs that block albumin binding to hFcRn. (FcRn 抗体)

SPR

Immunofluorescence staining of huSSECTM cell line overexpressing hFcRn-EGFP using clone ADM31 at 15µg/ml and Texas Red™ conjugated anti-mouse secondary. (FcRn 抗体)

Flowcytometry of huSSECTM cell line overexpressing hFcRn-EGFP labeled with clone ADM31 primary Ab and Texas Red™ conjugated anti-mouse secondary. (FcRn 抗体)

FACS

8 images

(15 references)

产品编号 ABIN1774762

发货至: 中国

Datasheet as PDF

Key Features
- High-quality FcRn antibody (Clone ADM31) for the detection of FcRn.
- Highly specific for human FcRn, blocks the binding of human serum albumin.
- PBS-only, preservative free
- 15 PubMed references available for this FcRn antibody.
抗原 See all FcRn 抗体

FcRn (neonatal Fc Receptor (FcRn))
适用
41

5

4

1

1

1

1

1
人
宿主
40

7

1
小鼠
Compatible Secondaries
克隆类型
40

7

1
单克隆
标记
24

5

5

5

2

1

1

1

1

1

1

1
This FcRn antibody is un-conjugated
应用范围
28

25

7

4

3

2

2

2

2

2

1

1

1

1

1

1
ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Cell-ELISA (cELISA), Functional Studies (Func)

产品特性

based on recognition of the complete native protein expressed on transfected mammalian cells

纯化方法

Purified, Protein G

免疫原

hFcRn

克隆位点

ADM31

亚型

IgG2b lambda

Product Specific Information

What can the FcRn antibody ABIN1774762 / ADM31 antibody be used for? This monoclonal mouse anti-FcRn antibody is unconjugated and is suitable for the detection of FcRn (Fc receptor) in human samples. The FcRn antibody is validated for Cell ELISA, ELISA, FACS and IF.

What validation data is available for this FcRn antibody / ADM31 antibody? 6 images for FACS and other applications are available. The product is cited in 15 PubMed publications. User our high quality product to reliably detect Fc receptor.

The MHC class I-like Fc receptor (FcRn) functions as an intracellular trafficking receptor, uniquely accountable for prolonging the serum half-life of IgG subclass antibodies and facilitating their passage across cellular barriers. Through these actions, FcRn significantly impacts various aspects of antibody biology and pathobiology. Its crucial role in regulating IgG pharmacokinetics has been exploited in the development of therapeutic antibodies and related biological treatments. Additionally, FcRn is involved in the transport of serum albumin, contributing to the improved pharmacokinetic characteristics of albumin-conjugated therapies. However, a lack of dependable serological tools targeting human FcRn has limited the comprehension of its functions and potential therapeutic uses.

Limited uses of our FcRn antibody ABIN1774762 / ADM31
anti-neonatal Fc Receptor (FcRn) antibody
FcRn 适用: 人 ELISA, IF, FACS, cELISA 宿主: 小鼠 Monoclonal DVN24 unconjugated

anti-neonatal Fc Receptor (FcRn) (AA 24-297) antibody
FcRn 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-neonatal Fc Receptor (FcRn) (AA 24-365) antibody
FcRn 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-neonatal Fc Receptor (FcRn) (AA 24-215) antibody
FcRn 适用: 人 IHC, ISt, StM 宿主: 小鼠 Monoclonal FCGRT-2932 unconjugated

anti-neonatal Fc Receptor (FcRn) (AA 24-297) antibody
FcRn 适用: 人 ELISA, FACS 宿主: 小鼠 Monoclonal 2B3B11 unconjugated

anti-neonatal Fc Receptor (FcRn) (AA 24-297) antibody
FcRn 适用: 人 ELISA, FACS 宿主: 小鼠 Monoclonal 5F3D1 unconjugated

anti-neonatal Fc Receptor (FcRn) (AA 25-300) antibody
FcRn 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated

anti-neonatal Fc Receptor (FcRn) (N-Term) antibody
FcRn 适用: 人, 小鼠, Cow, 犬, 豚鼠, 马, 绵羊 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-neonatal Fc Receptor (FcRn) (N-Term) antibody
FcRn 适用: 人 WB 宿主: 兔 Polyclonal unconjugated

anti-neonatal Fc Receptor (FcRn) (N-Term) antibody
FcRn 适用: 人 WB 宿主: 兔 Polyclonal unconjugated

应用备注
- Flow cytometry: 1.2 μg/10^6 cells
- ELISA: 1:200 - 1:400
- CELISA: 1:200 - 1:400
For each application a titration should be performed to determine the optimal concentration.
限制

仅限研究用
缓冲液

PBS pH 7.2

注意事项

avoid repeated freezing and thawing

储存条件

4 °C
Gjølberg, Frick, Mester, Foss, Grevys, Høydahl, Jørstad, Schlothauer, Sandlie, Moe, Andersen: "Biophysical differences in IgG1 Fc-based therapeutics relate to their cellular handling, interaction with FcRn and plasma half-life." in: Communications biology, Vol. 5, Issue 1, pp. 832, (2022) (PubMed).

Cines, Zaitsev, Rauova, Rux, Stepanova, Krishnaswamy, Sarkar, Kowalska, Zhao, Mast, Blumberg, McCrae, Poncz, Hubbard, Pyzik, Blumberg: "FcRn augments induction of tissue factor activity by IgG-containing immune complexes." in: Blood, Vol. 135, Issue 23, pp. 2085-2093, (2021) (PubMed).

Hubbard, Pyzik, Rath, Kozicky, Sand, Gandhi, Grevys, Foss, Menzies, Glickman, Fiebiger, Roopenian, Sandlie, Andersen, Sly, Baker, Blumberg: "FcRn is a CD32a coreceptor that determines susceptibility to IgG immune complex-driven autoimmunity." in: The Journal of experimental medicine, Vol. 217, Issue 10, (2021) (PubMed).

Bern, Nilsen, Ferrarese, Sand, Gjølberg, Lode, Davidson, Camire, Bækkevold, Foss, Grevys, Dalhus, Wilson, Høydahl, Christianson, Roopenian, Schlothauer, Michaelsen, Moe, Lombardi, Pinotti, Sandlie et al.: "An engineered human albumin enhances half-life and transmucosal delivery when fused to protein-based biologics. ..." in: Science translational medicine, Vol. 12, Issue 565, (2021) (PubMed).

Casulleras, Flores-Costa, Duran-Güell, Alcaraz-Quiles, Sanz, Titos, López-Vicario, Fernández, Horrillo, Costa, de la Grange, Moreau, Arroyo, Clària: "Albumin internalizes and inhibits endosomal TLR signaling in leukocytes from patients with decompensated cirrhosis." in: Science translational medicine, Vol. 12, Issue 566, (2021) (PubMed).

Blumberg, Humphries, Jones, Pearce, Holgate, Hearn, Cheung, Mahmood, Del Tito, Graydon, Stolz, Bitonti, Purohit, de Graaf, Kacena, Andersen, Christianson, Roopenian, Hubbard, Gandhi, Lasseter, Pyzik et al.: "Blocking FcRn in humans reduces circulating IgG levels and inhibits IgG immune complex-mediated immune responses. ..." in: Science advances, Vol. 5, Issue 12, pp. eaax9586, (2020) (PubMed).

Chung, Nguyen, Lin, Lafrance-Vanasse, Scales, Lin, Deng, Williams, Sperinde, Li, Zheng, Sukumaran, Tesar, Ernst, Fischer, Lazar, Prabhu, Song: "An in vitro FcRn- dependent transcytosis assay as a screening tool for predictive assessment of nonspecific clearance of antibody therapeutics in humans." in: mAbs, Vol. 11, Issue 5, pp. 942-955, (2019) (PubMed).

Swiercz, Mo, Khare, Schneider, Ober, Ward: "Loss of expression of the recycling receptor, FcRn, promotes tumor cell growth by increasing albumin consumption." in: Oncotarget, Vol. 8, Issue 2, pp. 3528-3541, (2018) (PubMed).

Pyzik, Rath, Kuo, Win, Baker, Hubbard, Grenha, Gandhi, Krämer, Mezo, Taylor, McDonnell, Nienaber, Andersen, Mizoguchi, Blumberg, Purohit, Jones, Christianson, Lencer, Sandlie, Kaplowitz, Roopenian et al.: "Hepatic FcRn regulates albumin homeostasis and susceptibility to liver injury. ..." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 114, Issue 14, pp. E2862-E2871, (2018) (PubMed).

Grevys, Nilsen, Sand, Daba, Øynebråten, Bern, McAdam, Foss, Schlothauer, Michaelsen, Christianson, Roopenian, Blumberg, Sandlie, Andersen: "A human endothelial cell-based recycling assay for screening of FcRn targeted molecules." in: Nature communications, Vol. 9, Issue 1, pp. 621, (2018) (PubMed).

Roopenian, Christianson, Proetzel, Sproule: "Human FcRn Transgenic Mice for Pharmacokinetic Evaluation of Therapeutic Antibodies." in: Methods in molecular biology (Clifton, N.J.), Vol. 1438, pp. 103-14, (2017) (PubMed).

Foss, Grevys, Sand, Bern, Blundell, Michaelsen, Pleass, Sandlie, Andersen: "Enhanced FcRn-dependent transepithelial delivery of IgG by Fc-engineering and polymerization." in: Journal of controlled release : official journal of the Controlled Release Society, Vol. 223, pp. 42-52, (2016) (PubMed).

Christianson, Sun, Akilesh, Pesavento, Proetzel, Roopenian: "Monoclonal antibodies directed against human FcRn and their applications." in: mAbs, Vol. 4, Issue 2, pp. 208-16, (2014) (PubMed).

Powner, McKenzie, Christianson, Roopenian, Fruttiger: "Expression of neonatal Fc receptor in the eye." in: Investigative ophthalmology & visual science, Vol. 55, Issue 3, pp. 1607-15, (2014) (PubMed).

Sand, Dalhus, Christianson, Bern, Foss, Cameron, Sleep, Bjørås, Roopenian, Sandlie, Andersen: "Dissection of the neonatal Fc receptor (FcRn)-albumin interface using mutagenesis and anti-FcRn albumin-blocking antibodies." in: The Journal of biological chemistry, Vol. 289, Issue 24, pp. 17228-39, (2014) (PubMed).
抗原

FcRn (neonatal Fc Receptor (FcRn))

Abstract

FcRn 产品

背景

The MHC class I-like Fc receptor (FcRn) is an intracellular trafficking Fc receptor that is uniquely responsible for the extended serum half-life of antibodies of the IgG subclass and their ability to transport across cellular barriers. By performing these functions, FcRn affects numerous facets of antibody biology and pathobiology. Its critical role in controlling IgG pharmacokinetics has been leveraged for the design of therapeutic antibodies and related biologics. FcRn also traffics serum albumin and is responsible for the enhanced pharmacokinetic properties of albumin-conjugated therapeutics.
Synonyms: IgG receptor FcRn large subunit p51, Neonatal Fc receptor, FCGRT

UniProt

P55899

途径

Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process