Fc Receptor (FcRn) 抗体

产品细节 (hide)

抗原

Fc Receptor (FcRn)

适用 All reactivities for Fc Receptor (FcRn) 抗体

• Human 1
• Mouse 1

人

宿主 All hosts for Fc Receptor (FcRn) 抗体

• Mouse 1

小鼠

Compatible Secondaries

克隆类型 All clonalities for Fc Receptor (FcRn) 抗体

• Monoclonal 1

单克隆

标记 All conjugates for Fc Receptor (FcRn) 抗体

• 非结合性 1

非结合性

应用范围

Cell-ELISA (cELISA), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Functional Studies (Func)

产品特性

based on recognition of the complete native protein expressed on transfected mammalian cells

纯化方法

Purified, Protein A

免疫原

Antibodies were generated by immunizing mouse FcRn-deficient mice with spleen cells from mice transgenic for human FcRn.

克隆位点

ADM31

亚型

IgG2b

Alternatives (show)

anti-Fc Receptor (FcRn) antibody

适用: 人, 小鼠 cELISA, ELISA, FACS, IF 宿主: 小鼠 Monoclonal DVN24 unconjugated

使用细节 (hide)

应用备注

• Flow cytometry: 1.2 μg/10^6 cells
• ELISA: 1:200 - 1:400
• CELISA: 1:200 - 1:400

For each application a titration should be performed to determine the optimal concentration.

限制

仅限研究用

贮存及处理 (hide)

缓冲液

phosphate buffered saline, pH 7.2

注意事项

avoid repeated freezing and thawing

储存条件

4 °C

引用 (hide)

Gjølberg, Frick, Mester, Foss, Grevys, Høydahl, Jørstad, Schlothauer, Sandlie, Moe, Andersen: "Biophysical differences in IgG1 Fc-based therapeutics relate to their cellular handling, interaction with FcRn and plasma half-life." in: Communications biology, Vol. 5, Issue 1, pp. 832, (2022) (PubMed).

Cines, Zaitsev, Rauova, Rux, Stepanova, Krishnaswamy, Sarkar, Kowalska, Zhao, Mast, Blumberg, McCrae, Poncz, Hubbard, Pyzik, Blumberg: "FcRn augments induction of tissue factor activity by IgG-containing immune complexes." in: Blood, Vol. 135, Issue 23, pp. 2085-2093, (2021) (PubMed).

Hubbard, Pyzik, Rath, Kozicky, Sand, Gandhi, Grevys, Foss, Menzies, Glickman, Fiebiger, Roopenian, Sandlie, Andersen, Sly, Baker, Blumberg: "FcRn is a CD32a coreceptor that determines susceptibility to IgG immune complex-driven autoimmunity." in: The Journal of experimental medicine, Vol. 217, Issue 10, (2021) (PubMed).

Bern, Nilsen, Ferrarese, Sand, Gjølberg, Lode, Davidson, Camire, Bækkevold, Foss, Grevys, Dalhus, Wilson, Høydahl, Christianson, Roopenian, Schlothauer, Michaelsen, Moe, Lombardi, Pinotti, Sandlie et al.: "An engineered human albumin enhances half-life and transmucosal delivery when fused to protein-based biologics. ..." in: Science translational medicine, Vol. 12, Issue 565, (2021) (PubMed).

Casulleras, Flores-Costa, Duran-Güell, Alcaraz-Quiles, Sanz, Titos, López-Vicario, Fernández, Horrillo, Costa, de la Grange, Moreau, Arroyo, Clària: "Albumin internalizes and inhibits endosomal TLR signaling in leukocytes from patients with decompensated cirrhosis." in: Science translational medicine, Vol. 12, Issue 566, (2021) (PubMed).

Blumberg, Humphries, Jones, Pearce, Holgate, Hearn, Cheung, Mahmood, Del Tito, Graydon, Stolz, Bitonti, Purohit, de Graaf, Kacena, Andersen, Christianson, Roopenian, Hubbard, Gandhi, Lasseter, Pyzik et al.: "Blocking FcRn in humans reduces circulating IgG levels and inhibits IgG immune complex-mediated immune responses. ..." in: Science advances, Vol. 5, Issue 12, pp. eaax9586, (2020) (PubMed).

Chung, Nguyen, Lin, Lafrance-Vanasse, Scales, Lin, Deng, Williams, Sperinde, Li, Zheng, Sukumaran, Tesar, Ernst, Fischer, Lazar, Prabhu, Song: "An in vitro FcRn- dependent transcytosis assay as a screening tool for predictive assessment of nonspecific clearance of antibody therapeutics in humans." in: mAbs, Vol. 11, Issue 5, pp. 942-955, (2019) (PubMed).

Swiercz, Mo, Khare, Schneider, Ober, Ward: "Loss of expression of the recycling receptor, FcRn, promotes tumor cell growth by increasing albumin consumption." in: Oncotarget, Vol. 8, Issue 2, pp. 3528-3541, (2018) (PubMed).

Pyzik, Rath, Kuo, Win, Baker, Hubbard, Grenha, Gandhi, Krämer, Mezo, Taylor, McDonnell, Nienaber, Andersen, Mizoguchi, Blumberg, Purohit, Jones, Christianson, Lencer, Sandlie, Kaplowitz, Roopenian et al.: "Hepatic FcRn regulates albumin homeostasis and susceptibility to liver injury. ..." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 114, Issue 14, pp. E2862-E2871, (2018) (PubMed).

Grevys, Nilsen, Sand, Daba, Øynebråten, Bern, McAdam, Foss, Schlothauer, Michaelsen, Christianson, Roopenian, Blumberg, Sandlie, Andersen: "A human endothelial cell-based recycling assay for screening of FcRn targeted molecules." in: Nature communications, Vol. 9, Issue 1, pp. 621, (2018) (PubMed).

Roopenian, Christianson, Proetzel, Sproule: "Human FcRn Transgenic Mice for Pharmacokinetic Evaluation of Therapeutic Antibodies." in: Methods in molecular biology (Clifton, N.J.), Vol. 1438, pp. 103-14, (2017) (PubMed).

Foss, Grevys, Sand, Bern, Blundell, Michaelsen, Pleass, Sandlie, Andersen: "Enhanced FcRn-dependent transepithelial delivery of IgG by Fc-engineering and polymerization." in: Journal of controlled release : official journal of the Controlled Release Society, Vol. 223, pp. 42-52, (2016) (PubMed).

Christianson, Sun, Akilesh, Pesavento, Proetzel, Roopenian: "Monoclonal antibodies directed against human FcRn and their applications." in: mAbs, Vol. 4, Issue 2, pp. 208-16, (2014) (PubMed).

Powner, McKenzie, Christianson, Roopenian, Fruttiger: "Expression of neonatal Fc receptor in the eye." in: Investigative ophthalmology & visual science, Vol. 55, Issue 3, pp. 1607-15, (2014) (PubMed).

Sand, Dalhus, Christianson, Bern, Foss, Cameron, Sleep, Bjørås, Roopenian, Sandlie, Andersen: "Dissection of the neonatal Fc receptor (FcRn)-albumin interface using mutagenesis and anti-FcRn albumin-blocking antibodies." in: The Journal of biological chemistry, Vol. 289, Issue 24, pp. 17228-39, (2014) (PubMed).

目标详细情况 (hide)

抗原

Fc Receptor (FcRn)

背景

The MHC class I-like Fc receptor (FcRn) is an intracellular trafficking Fc receptor that is uniquely responsible for the extended serum half-life of antibodies of the IgG subclass and their ability to transport across cellular barriers. By performing these functions, FcRn affects numerous facets of antibody biology and pathobiology. Its critical role in controlling IgG pharmacokinetics has been leveraged for the design of therapeutic antibodies and related biologics. FcRn also traffics serum albumin and is responsible for the enhanced pharmacokinetic properties of albumin-conjugated therapeutics.
Synonyms: IgG receptor FcRn large subunit p51, Neonatal Fc receptor, FCGRT

UniProt

P55899

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