Enzyme-linked immunosorbent assay (ELISA) is proven, widely-used solid-phase immunoassay method that is used for detecting or quantifying one or several analytes in an aqueous sample. ELISA is most frequently used when the researcher needs to rapidly detect or quantify the presence of a single target in a complex biological matrix (e.g. plasma, serum, tissue homogenate).
If you need to perform an ELISA, an ELISA kit is a good option because: 1. The kit contains all of the necessary reagents in one box – no need to buy anything extra. 2. Individual components are already tested, and have been proven effective and compatible with each-other. 3. ELISA Kits are simple to use, with step-by-step instructions, so that even individuals with minimal training can complete the assay with minimal oversight. 4. The cost-per-sample is generally lower than for de novo assay generation.