Lateral flow tests are rapid immunochromatographic tests in strip format. They enable the detection of diverse analytes within just a few minutes. In addition to rapid results and ease of use, the advantages of lateral flow tests are low cost and high validity. Since they can be performed without complex infrastructure, they have versatile applications as point-of-care tests (POCT). antibodies-online offers a wide range of high quality antibodies suitable for utilization in lateral flow assays.
What is a Lateral Flow Test?
Lateral Flow Assays provide a simple and fast way to test a liquid patient sample, such as blood, urine, serum, saliva, sweat or other fluids, for a specific analyte. The first lateral flow test that entered the market was the Clearblue 1-step test in 1988 as a home test for the detection of the pregnancy hormone. In the past years, the technology has been utilized to great success in detection of infectious diseases, especially SARS-CoV-2.
How does a Lateral Flow Immunoassay Work?
Lateral flow tests are another variation of the ELISA in which antigens are immobilized on a strip of cellulose or other suitable matrix. As with other immunological assays, the test method is based on an antigen-antibody reaction.The sample is applied at one end of the strip and any antibodies present diffuse along it until they reach the position where the appropriate antigen is immobilized. Antibodies specific for the antigen bind to and remain at the site of the antigen and are visualized using labeled detection antibody.
Usually, a positive control antigen is placed on the strip so that it is encountered after the test antigen to confirm that the sample has diffused a sufficient distance to interact with the test antigen. More than one test antigen can be dotted on the strip to provide a multiplex test.
Lateral flow tests can be used both qualitatively and quantitatively. This is done by interpreting the band intensity of the analyte using an optical reader.
Application Fields for Lateral Flow Assays
Lateral flow tests are widely across many industries sectors for point of care testing, including pharma, environmental testing, animal health, food and feed testing, and plant and crop health. As they can be performed by professionals, trained users or even patients, regardless of the exquipment, lateral flow immunoassays are of particular value in medical diagnostic. Lateral flow tests are the most used type of rapid diagnostic test, a medical diagnostic test that is quick and easy to perform and used to confirm the presence or absence of a target analyte, such as pathogens or biomarkers in humans or animals.
Antibodies for Lateral Flow Assay Development
antibodies-online offers a wide range of high quality antibodies suitable for utilization in lateral flow assays. The focus of our portfolio lies on antibodies against compounds of the human body as well as various viruses. In addition, we also offer antibodies against viruses, fungi or bacteria. Discover our complete portfolio down below.
All Lateral Flow Antibodies