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RFP 抗体

Name: RFP 抗体
Brand: antibodies-online
SKU: ABIN1607680
Availability: OutOfStock

RFP 适用: Discosoma WB, ELISA 宿主: 小鼠 Monoclonal 8E5-G7 unconjugated

Western Blot of Mouse Anti-RFP Antibody Western Blot of Mouse Anti-RFP Antibody. (RFP 抗体)

Characterization of the transgenic model and effect of 4D on the RMSAFluorescence image of a sagittal section of a 4D+ brain after a 4-day treatment with doxycycline showing RFP signal confined to the SVZ and RMS (nuclei counterstained with DAPI, blue). (RFP 抗体)

IF (p)

ELISA

Western Blot of Mouse Anti-RFP antibody. (RFP 抗体)

Transgenic model and effects of acute 4D overexpression on NSC and progenitors of the SVZDrawings of the nestinCreERT2 (Imayoshi et al, 2008), ROSA26rtTA-flox (Belteki et al, 2005), and tet4D-RFP (Nonaka-Kinoshita et al, 2013) alleles of the 4D line. (RFP 抗体)

IF (p)

Chronic effect of 4D overexpression on NSC and OB neurogenesisAExperimental design used to assess the chronic effect of a transient 4D induction with BrdU and EdU given during Dox administration or 1 h before sacrifice, respectively. (RFP 抗体)

IF (p)

9 images

(16 references)

产品编号 ABIN1607680

发货至: 中国

Datasheet as PDF

抗原 See all RFP 抗体

RFP (Red Fluorescent Protein (RFP))

适用

Discosoma
宿主
23

12

9

5

3
小鼠
Compatible Secondaries
克隆类型
29

23
单克隆
标记
18

5

3

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This RFP antibody is un-conjugated
应用范围
26

21

14

12

10

7

6

6

2

1

1

1
Western Blotting (WB), ELISA

特异性

Expect reactivity against RFP and its variants: mCherry, tdTomato, mBanana, mOrange, mPlum, mOrange and mStrawberry.

纯化方法

Anti-RFP Monoclonal Antibody was purified from concentrated tissue culture supernate by Protein A chromatography.

免疫原

Anti-RFP monoclonal antibody is a Red Fluorescent Protein (RFP) fusion protein corresponding to the full length amino acid sequence (234aa) derived from the mushroom polyp coral Discosoma.
Immunogen Type: Recombinant Protein

克隆位点

8E5-G7

亚型

IgG2a

Featured

Discover our best selling RFP Primary Antibody

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Discover our top product RFP Primary Antibody
anti-Red Fluorescent Protein (RFP) antibody
RFP 适用: Discosoma WB, ELISA, IF, IP, IHC, FACS, IHC (fro), IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Red Fluorescent Protein (RFP) antibody
RFP 适用: Discosoma WB, ELISA 宿主: 小鸡 Polyclonal unconjugated

anti-Red Fluorescent Protein (RFP) antibody
RFP 适用: Discosoma WB, IF, IHC (fro), IHC (p) 宿主: 山羊 Polyclonal unconjugated

anti-Red Fluorescent Protein (RFP) antibody (Biotin)
RFP 适用: Discosoma WB, ELISA, IF 宿主: 兔 Polyclonal Biotin

anti-Red Fluorescent Protein (RFP) antibody (Biotin)
RFP 适用: Discosoma WB, ELISA 宿主: 小鸡 Polyclonal Biotin

anti-Red Fluorescent Protein (RFP) antibody (DyLight 633)
RFP 适用: Discosoma WB, IF, IHC (fro) 宿主: 山羊 Polyclonal DyLight 633

anti-Red Fluorescent Protein (RFP) antibody (DyLight 550)
RFP 适用: Discosoma WB, IF, IHC (fro) 宿主: 山羊 Polyclonal DyLight 550

anti-Red Fluorescent Protein (RFP) antibody (DyLight 488)
RFP 适用: Discosoma WB, IF, IHC (fro) 宿主: 山羊 Polyclonal DyLight 488

anti-Red Fluorescent Protein (RFP) antibody (HRP)
RFP 适用: Discosoma WB, ELISA, IF 宿主: 兔 Polyclonal HRP

anti-Red Fluorescent Protein (RFP) antibody (DyLight 405)
RFP 适用: Discosoma WB, IF, IHC (fro) 宿主: 山羊 Polyclonal DyLight 405

应用备注

Anti-RFP antibodies is designed to detect Red Fluorescent Protein and its variants. This antibody can be used to detect RFP by ELISA (sandwich or capture) for the direct binding of antigen. Biotin conjugated anti-RFP used in a sandwich ELISA with unconjugated anti-RFP is well suited to titrate RFP in solution. The detection antibody conjugated to biotin is subsequently reacted with streptavidin conjugated HRP. Fluorochrome conjugated anti-RFP can be used to detect RFP by immunofluorescence microscopy in cell expression systems and can detect RFP containing inserts. Significant amplification of signal is achieved using fluorochrome conjugated anti-RFP relative to the fluorescence of RFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated anti-RFP to detect RFP or RFP containing proteins on western blots. Optimal titers for applications should be determined by the researcher.
ELISA Dilution: 1:20.000-1:50.000
Western Blot Dilution: 1:1000-1:3000

限制

仅限研究用
状态

Liquid

浓度

1 mg/mL

缓冲液

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

注意事项

Avoid cycles of freezing and thawing.

储存条件

4 °C/-20 °C

储存方法

Store vial at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use.

有效期

Expiration date is one (1) year from date of opening.
Singh, Elenio, Leu, Romano, Vaughan, DeRiso, Surendran, Chakrabarti: "A new Elf5CreERT2-GFP BAC transgenic mouse model for tracing Elf5 cell lineages in adult tissues." in: FEBS letters, Vol. 593, Issue 10, pp. 1030-1039, (2020) (PubMed).

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Nicholson, Roberts, Sober: "Thalamostriatal and cerebellothalamic pathways in a songbird, the Bengalese finch." in: The Journal of comparative neurology, Vol. 526, Issue 9, pp. 1550-1570, (2019) (PubMed).

Bragado Alonso, Reinert, Marichal, Massalini, Berninger, Kuner, Calegari: "An increase in neural stem cells and olfactory bulb adult neurogenesis improves discrimination of highly similar odorants." in: The EMBO journal, Vol. 38, Issue 6, (2019) (PubMed).

Zhou, Rickmann, Hafner, Staiger: "Subcellular Targeting of VIP Boutons in Mouse Barrel Cortex is Layer-Dependent and not Restricted to Interneurons." in: Cerebral cortex (New York, N.Y. : 1991), Vol. 27, Issue 11, pp. 5353-5368, (2018) (PubMed).

Rider, Cheerathodi, Hurwitz, Nkosi, Howell, Tremblay, Liu, Zhu, Meckes: "The interactome of EBV LMP1 evaluated by proximity-based BioID approach." in: Virology, Vol. 516, pp. 55-70, (2018) (PubMed).

Guan, Cao, Liu, Zhao, Fu, Yu: "Eye opening differentially modulates inhibitory synaptic transmission in the developing visual cortex." in: eLife, Vol. 6, (2018) (PubMed).

Hou, Zhang: "NMDA Receptors Regulate the Development of Neuronal Intrinsic Excitability through Cell-Autonomous Mechanisms." in: Frontiers in cellular neuroscience, Vol. 11, pp. 353, (2017) (PubMed).

Bifari, Decimo, Pino, Llorens-Bobadilla, Zhao, Lange, Panuccio, Boeckx, Thienpont, Vinckier, Wyns, Bouché, Lambrechts, Giugliano, Dewerchin, Martin-Villalba, Carmeliet: "Neurogenic Radial Glia-like Cells in Meninges Migrate and Differentiate into Functionally Integrated Neurons in the Neonatal Cortex." in: Cell stem cell, Vol. 20, Issue 3, pp. 360-373.e7, (2017) (PubMed).

Meese, Cepeda, Gahlen, Adams, Ficner, Ricci, Heller, Reisinger, Herget: "Activity-Dependent Phosphorylation by CaMKIIδ Alters the Ca2+ Affinity of the Multi-C2-Domain Protein Otoferlin." in: Frontiers in synaptic neuroscience, Vol. 9, pp. 13, (2017) (PubMed).

Clarkson, Liptak, Gee, Sohal, Bender: "D3 Receptors Regulate Excitability in a Unique Class of Prefrontal Pyramidal Cells." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 37, Issue 24, pp. 5846-5860, (2017) (PubMed).

Wu, Kim, Seravalli, Barycki, Hart, Gohara, Di Cera, Jung, Kosman, Lee: "Potassium and the K+/H+ Exchanger Kha1p Promote Binding of Copper to ApoFet3p Multi-copper Ferroxidase." in: The Journal of biological chemistry, Vol. 291, Issue 18, pp. 9796-806, (2016) (PubMed).

Alexandre, Ghilas, Sanchez, Le Bon, Crozat, Dalod: "XCR1+ dendritic cells promote memory CD8+ T cell recall upon secondary infections with Listeria monocytogenes or certain viruses." in: The Journal of experimental medicine, Vol. 213, Issue 1, pp. 75-92, (2016) (PubMed).
抗原

RFP (Red Fluorescent Protein (RFP))

Abstract

RFP 产品

背景

Antibodies to RFP (Discosoma spp.) are intended for use in immunological assays including ELISA, western blotting, fluorometry and fluorescence activated cell sorting (FACS). Synonyms: DsRed, rDsRed, Discosoma sp. Red Fluorescent Protein, Red fluorescent protein drFP583