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Independently validated

Here you can find our antibodies which have been validated through our Independent Validation Initiative(IVI).

The IVI is centered around core values of transparency, reproducibility, scientific integrity. We are committed to improving the antibody and antibody market by providing independently collected, scientifically sound, and properly documented data for the products that we sell, which is why we set very high standards for independent validation data. Our support can answer further questions via phone, live chat or email.

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218 results:

anti-RFP 抗体

RFP 适用: Discosoma ELISA, FACS, IF, IHC, IHC (fro), IHC (p), IP, WB 宿主: 兔 Polyclonal unconjugated

Immunofluorescence validation image for anti-Red Fluorescent Protein (RFP) antibody (ABIN1043867)

Western Blot of Rabbit anti-RFP antibody.

Immunofluorescence Microscopy of Rabbit Anti-RFP antibody.

Immunofluorescence Microscopy of Rabbit Anti-RFP antibody.

Immunofluorescence Microscopy of Rabbit Anti-RFP antibody.

Immunofluorescence Microscopy of Rabbit Anti-RFP antibody.

Immunohistochemistry of Anti-RFP Antibody.

Western Blot of Rabbit Anti-RFP MW hu, ms, rt Antibody.

Western blot of RFP recombinant protein detected with polyclonal anti-RFP antibody.

Western Blot of Rabbit anti-RFP Antibody.

ELISA Results of Polyclonal Rabbit Anti-RFP Antibody tested against purified RFP protein.

Western Blot of RFP Antibody Pre-Absorbed.

12 images

353 references

Independent Validation

1 validation

产品编号 ABIN129578

$350.00

Free shipping!

100 μL (More quantities available)

发货至: 美国

1至2个工作日

anti-Collagen Type I 抗体

COL1 适用: 人, 小鼠, 大鼠, Cow, 哺乳动物 ELISA, IHC, IP, WB 宿主: 兔 Polyclonal unconjugated

The testing of multi-differentiation of rabbit TSCs in vivo.

Immunofluorescence staining of extracellular and intracellular type I collagen.

Western blot analysis is shown using Affinity Purified anti-Collagen I antibody to detect expression of collagen I in Wistar rat hepatic stellate cells (HSC) in control (GFP-transduced) (left lane) and PPARg-transduced cell lysates (right lane).

Reduced secretion of type I collagen by Akt inhibition and S451A overexpression.

Anti-fibrotic effect of apigenin in human HSC line (TWNT-4 cells).

ASH1 positively influences the expression of TIMP-1, TGF-β-1, and Collagen I.

LPA increases α-SMA and ECM production in HTM cells.

AICAR treatment reduces post-MI adverse remodeling.

SMT022357 improves the pathology in the diaphragm.

Histological analysis of muscle and tendon monoculture tissue model differentiation.

CYD0682 suppresses endogenous ECM protein expression.

Collagen type I expression in the IVD tissue.

Both serum-free and serum-containing fibrocytes express both haematopoietic and stromal cell markers.

The augmented participation from infiltrating monocytes into myeloid-dependent fibrosis in the aging B6J heart.

Immunoperoxidase stain for collagen type I (a–c), collagen type III (d–f) and collagen type VI (g–i).

At least two grid per tissue biopsies were prepared.

Collagen Far Western blot of B.

Immunohistochemistry of Collagen I antibody.

Immunohistochemistry of Collagen I antibody Tissue: human liung Fixation: formalin fixed paraffin embedded Antigen retrieval: user optimized Primary antibody: Collagen 1 1:400 Secondary antibody: Peroxidase goat anti-rabbit at 1:10,000 for 45 min at RT Localization: Strong staining was observed in the extracellular matrix of the lung.

Effect of ex vivo treatment with Pirfenidone and Nintedanib on the fibrotic phenotype of 3D-LTCs.

SIM mutant as dominant negative protein for type I collagen expression.

Transgenic VEGFA overexpression reduced bleomycin-induced lung fibrosis.

Zoledronic acid (ZA) at the maximum concentration in serum (Cmax) inhibits the transforming growth factor-β (TGF-β)-induced myofibroblast (MF) differentiation of human gingival fibroblasts (hGFs).

HJC0123 inhibits hepatic stellate cells activation and decreases endogenous ECM proteins expression.

Recombinant ACLP inhibits adipogenesis and enhances myofibroblast differentiation in human adipose stromal cells.

Age-related alterations in collagen expression and structure.

CCR2 mediates the specific accumulation of type I collagen positive exudate macrophages and Ly-6Chigh monocytes in mice with targeted type II AEC injury.

Oridonin suppresses α-SMA and ECM protein expression.

Immunohistochemistry of Rabbit Anti-collagen type I antibody.

CYD0692 suppresses endogenous and TGF-β induced ECM protein expression.

Immunofluorescence and SHG detection of collagens in NAFLD stage 0 fibrosis.

Inhibition of mTORC1 reduces secretion of type I collagen.

p21 knockout alleviates liver fibrosis.

Decreased gene and protein expression of Tnmd in Scx-deficient mice.

Representative histological analysis for collagen quantification of (a) native and (b) decellularized equine lung tissue.

Western blot of Human Collagen Type I.

Expression of collagen by 5 day cultured spleen cells.

Pulmonary fibroblasts deposit elastin and collagen within Gelfoam matrices.

Inhibition of collagen prolyl hydroxylase by PDC significantly reduced collagen production in vitro and attenuated BLM-induced lung fibrosis in vivo.

Immunofluorescence staining of extracellular and intracellular type I collagen.

Immunofluorescence staining of extracellular and intracellular type I collagen.

42 images

62 references

Independent Validation

1 validation

产品编号 ABIN5596819

$548.90

加上运输费 $45.00

100 μg (More quantities available)

发货至: 美国

1至2个工作日

anti-DYKDDDDK Tag 抗体

IP, ELISA, WB 宿主: 兔 Polyclonal unconjugated

Western Blotting validation image for anti-DYKDDDDK Tag antibody (ABIN99294)

Immunofluorescence validation image for anti-DYKDDDDK Tag antibody (ABIN99294)

The P466L clinical mutation leads to RECQ4 mitochondrial accumulation.

Affinity Purified Antibody to detect conjugated proteins detects both C terminal linked and N terminal linked tagged recombinant proteins by western blot.

Western Blot of Rabbit anti-FLAG antibody.

antibody to detect conjugated proteins is shown to detect as little as 3 ng of amino-terminal tagged recombinant protein by western blot.

The P466L clinical mutation leads to RECQ4 mitochondrial accumulation.

The P466L clinical mutation leads to RECQ4 mitochondrial accumulation.

8 images

20 references

Independent Validation

2 validations

产品编号 ABIN99294

$284.90

Plus shipping costs $45.00 and $20.00 dry ice

250 μg (More quantities available)

发货至: 美国

1至2个工作日

anti-TNF alpha 抗体 (AA 181-235)

TNF alpha 适用: 人, 小鼠, 大鼠, 马, 兔 ELISA, IF (p), IHC (fro), IHC (p), WB 宿主: 兔 Polyclonal unconjugated

Immunofluorescence validation image for anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 181-235) antibody (ABIN677318)

Immunofluorescence validation image for anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 181-235) antibody (ABIN677318)

Immunofluorescence validation image for anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 181-235) antibody (ABIN677318)

Immunofluorescence validation image for anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 181-235) antibody (ABIN677318)

Immunofluorescence validation image for anti-Tumor Necrosis Factor alpha (TNF alpha) (AA 181-235) antibody (ABIN677318)

Mouse liver lysates probed with TNF alpha Polyclonal Antibody, unconjugated at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C.

Histology and immunofluorescence in rat corneal sections.

Formalin-fixed and paraffin embedded rat hippocampus labeled with Rabbit Anti-TNF alpha Polyclonal Antibody (ABIN677318) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Formalin-fixed and paraffin embedded rat colitis labeled with Rabbit Anti-TNF alpha Polyclonal Antibody (ABIN677318) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Formalin-fixed and paraffin embedded rat colitis labeled with Rabbit Anti-TNF alpha Polyclonal Antibody at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Protein expression of PPARγ, IL-6, and TNF-α in WAT after vibration for 8 weeks.

11 images

21 references

Independent Validation

1 validation

产品编号 ABIN677318

$384.62

加上运输费 $45.00

100 μL

发货至: 美国

4至6个工作日

anti-Caspase 8 抗体 (AA 411-482)

CASP8 适用: 人, 小鼠, 大鼠, 猴, Pig, 绵羊 ELISA, FACS, IF (cc), IF (p), IHC (fro), IHC (p), WB 宿主: 兔 Polyclonal unconjugated

Western Blotting validation image for anti-Caspase 8 (CASP8) (AA 411-482) antibody (ABIN724205)

Image provided by the Independent Validation Program (badge number 29759).

Formalin-fixed and paraffin embedded human rectal carcinoma labeled with Anti-Caspase-8 Polyclonal Antibody, Unconjugated (ABIN724205) 1:200 followed by conjugation to the secondary antibody and DAB staining

L1 mouse liver lysates L2 mouse spleen lysates probed with Anti Caspase 8 Polyclonal Antibody, Unconjugated (ABIN724205) at 1:200 overnight at 4 °C.

Image provided by One World Lab validation program.

Image provided by One World Lab validation program.

6 images

15 references

Independent Validation

1 validation

产品编号 ABIN724205

$384.62

加上运输费 $45.00

100 μL

发货至: 美国

4至6个工作日

anti-Iba1 抗体 (C-Term)

AIF1 适用: 人, 小鼠, 大鼠 IHC (fro), IHC (p), IHC, WB 宿主: 兔 Polyclonal unconjugated

Immunofluorescence validation image for anti-Allograft Inflammatory Factor 1 (AIF1) (C-Term) antibody (ABIN2857032)

WB Image Iba1 antibody detects Iba1 protein by western blot analysis.

IHC-P Image Iba1 antibody detects Iba1 protein on mouse fore brain by immunohistochemical analysis.

IHC-P Image Iba1 antibody detects Iba1 protein on rat hind brain by immunohistochemical analysis.

IHC-P Image Iba1 detects Iba1 protein at cytosol on mouse brain tissue by immunohistochemical analysis.

IHC-P Image Iba1 antibody detects Iba1 protein at cytoplasm on human colon carcinoma stroma by immunohistochemical analysis.

WB Image Sample (30 ug of whole cell lysate) A: Molt-4 , 15% SDS PAGE AIF1 antibody antibody diluted at 1:1000

IHC-P Image Immunohistochemical analysis of paraffin-embedded human hepatoma, using Iba1, antibody at 1:500 dilution.

WB Image Iba1 antibody detects Iba1 protein by Western blot analysis.

ASI administration deactivated microglial activation and reduced inflammatory responses in spinal cords of EAE mice.

Neuropathology in the central nervous system of Fuca1-deficient mice.

W20 attenuated neuropathology and gliosis in the brains of A53T α-synuclein mice.

W20 reduced neuropathology and neuroinflammation in BACHD mice.

Absence of neuroinflammation in RNase H2ΔGFAP mice.

Effects of NADPH oxidase subunit deletion on plasma TNFα levels and microglial activation after LPS injection.

DPI post-treatment attenuated microglia activation in brain after LPS injection.

Expression of microglial marker protein characteristic for the homeostatic or disease-associated stateAImmunoblot analysis of ApoE, CLEC7A, TREM2, and CD68 in lysates of acutely isolated microglia from Grn-/- and Grn+/+ mice (9 months of age, n = 3 per genotype, female).

Development of myeloma with associated spinal glial cell activation.

18 images

13 references

Independent Validation

1 validation

产品编号 ABIN2857032

$609.18

加上运输费 $45.00

100 μL

发货至: 美国

3至4个工作日

anti-Dendra 2 抗体

适用: Coral FACS, ICC, WB 宿主: 兔 Polyclonal unconjugated

Immunocytochemistry validation image for anti-Dendra 2 antibody (ABIN361314)

Localization of Notch::Dendracrispr (anti-Dendra (ABIN361314), green), Histone2B::RFP expressed under the neur promoter (blue) and Delta (anti-Delta, red) in wild-type SOP daughter cells (D, n=9) and in strat SOP daughter cells expressing pnrGAL4>AP-47dsRNA (E, n=20).

Single optical section images showing Dendra2-NTR (green), Tomato (red), Bhmt (blue), and DAPI (gray) expression in regenerating livers of the WT and lvl at R48h with control (top panel) and Mtz treatment (bottom panel).

WB: line1 – Plum protein 27 kDa, line2 – E.

Localization of NiDendra (anti-Dendra, green), Histone2B::RFP expressed under the neur promoter (blue) and Delta (anti-Delta, red) in wild-type SOP daughter cells and in strat SOP daughter cells expressing pnr-GAL4>AP-47dsRNA.

5 images

10 references

Independent Validation

1 validation

产品编号 ABIN361314

$417.45

Plus shipping costs $45.00 and $20.00 dry ice

100 μg

发货至: 美国

5至7个工作日

anti-DVL1 抗体 (AA 21-100)

DVL1 适用: 人, 小鼠, 大鼠 ELISA, FACS, IF (cc), IF (p), IHC (fro), IHC (p), WB 宿主: 兔 Polyclonal unconjugated

Immunohistochemistry validation image for anti-Dishevelled Segment Polarity Protein 1 (DVL1) (AA 21-100) antibody (ABIN670671)

Immunohistochemistry validation image for anti-Dishevelled Segment Polarity Protein 1 (DVL1) (AA 21-100) antibody (ABIN670671)

Immunohistochemistry validation image for anti-Dishevelled Segment Polarity Protein 1 (DVL1) (AA 21-100) antibody (ABIN670671)

Immunohistochemistry validation image for anti-Dishevelled Segment Polarity Protein 1 (DVL1) (AA 21-100) antibody (ABIN670671)

Images provided the Independent Validation Program (badge number 029657)Formalin-fixed and paraffin embedded human kidney labeled with Rabbit Anti-DVL1 Polyclonal Antibody (ABIN670671) at 1:250 overnight at room temperature followed by conjugation to secondary antibody.

Lane 1: rat brain lysates Lane 2: rat lung lysates probed with Anti DVL1/Dishevelled Polyclonal Antibody, Unconjugated (ABIN670671) at 1:200 in 4 °C.

6 images

1 reference

Independent Validation

1 validation

产品编号 ABIN670671

$384.62

加上运输费 $45.00

100 μL

发货至: 美国

4至6个工作日

anti-Iba1 抗体 (AA 51-147)

AIF1 适用: 人, 小鼠, 大鼠 ELISA, FACS, IF (cc), IF (p), IHC (fro), IHC (p), WB 宿主: 兔 Polyclonal unconjugated

Immunofluorescence validation image for anti-Allograft Inflammatory Factor 1 (AIF1) (AA 51-147) antibody (ABIN685477)

Immunofluorescence validation image for anti-Allograft Inflammatory Factor 1 (AIF1) (AA 51-147) antibody (ABIN685477)

Immunofluorescence validation image for anti-Allograft Inflammatory Factor 1 (AIF1) (AA 51-147) antibody (ABIN685477)

Immunofluorescence validation image for anti-Allograft Inflammatory Factor 1 (AIF1) (AA 51-147) antibody (ABIN685477)

Formalin-fixed and paraffin embedded mouse brain labeled with Anti-AIF1/IBa-1 Polyclonal Antibody, Unconjugated (ABIN685477) followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti-AIF1 Polyclonal Antibody, Unconjugated used at 1:200 dilution for 40 minutes at 37°C.

Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-AIF1 Polyclonal Antibody, unonjugated 1:200, 40 minutes at 37°C

Formalin-fixed and paraffin embedded rat brain labeled with Anti-AIF1/IBa-1 Polyclonal Antibody, Unconjugated (ABIN685477) followed by conjugation to the secondary antibody and DAB staining

BV-microglia stained with AIF1 Polyclonal Antibody at 1:100 dilution.

Independently Validated Antibody, image provided by Science Direct, badge number 029613:Frozen,OCT embedded mouse spleen labeled with Anti-AIF1 Polyclonal Antibody, Unconjugated (ABIN685477) at 1:100 followed by conjugation to the secondary antibody and DAB staining

Formalin-fixed and paraffin embedded mouse spinal cord stained with AIF1 Polyclonal Antibody at 1:100 dilution.

11 images

7 references

Independent Validation

1 validation

产品编号 ABIN685477

$384.62

加上运输费 $45.00

100 μL

发货至: 美国

4至6个工作日

anti-WNT2B 抗体 (AA 301-391)

WNT2B 适用: 小鼠, 大鼠 ELISA, IF (cc), IF (p), IHC (fro), IHC (p), WB 宿主: 兔 Polyclonal unconjugated

Immunohistochemistry validation image for anti-Wingless-Type MMTV Integration Site Family, Member 2B (WNT2B) (AA 301-391) antibody (ABIN675728)

Immunohistochemistry validation image for anti-Wingless-Type MMTV Integration Site Family, Member 2B (WNT2B) (AA 301-391) antibody (ABIN675728)

Immunohistochemistry validation image for anti-Wingless-Type MMTV Integration Site Family, Member 2B (WNT2B) (AA 301-391) antibody (ABIN675728)

Immunohistochemistry validation image for anti-Wingless-Type MMTV Integration Site Family, Member 2B (WNT2B) (AA 301-391) antibody (ABIN675728)

Independently Validated Antibody, image provided by Science Direct, badge number 029457:Formalin-fixed and paraffin embedded human kidney labeled with Anti-WNT2B Polyclonal Antibody, Unconjugated (ABIN675728) at 1:250 followed by conjugation to the secondary antibody and DAB staining

Lane 1: rat brain lysates Lane 2: mouse intestine lysates probed with Anti WNT2B AVPR2 Polyclonal Antibody, Unconjugated (ABIN675728) at 1:200 in 4 °C.

6 images

1 reference

Independent Validation

1 validation

产品编号 ABIN675728

$384.62

加上运输费 $45.00

100 μL

发货至: 美国

4至6个工作日

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