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Complement Factor H 抗体 (AA 20-270)

Name: Complement Factor H 抗体 (AA 20-270)
Brand: antibodies-online
SKU: ABIN3023097
Availability: OutOfStock
Rating: 5 (1 reviews)

CFH 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

Immunofluorescence validation image for anti-Complement Factor H (CFH) (AA 20-270) antibody (ABIN3023097)

Western blot analysis of extracts of HepG2 cell line, using CFH antibody.

Immunohistochemistry of paraffin-embedded human placenta using Complement factor H Rabbit pAb (ABIN3023096, ABIN3023097, ABIN3023098 and ABIN6219387) at dilution of 1:100 (40x lens).

IHC

Western blot analysis of extracts of Rat liver, using Complement factor H antibody (ABIN3023096, ABIN3023097, ABIN3023098 and ABIN6219387) at 1:1000 dilution.

Western blot analysis of extracts of Human plasma, using Complement factor H antibody (ABIN3023096, ABIN3023097, ABIN3023098 and ABIN6219387) at 1:1000 dilution.

Western blot analysis of extracts of various cell lines, using Complement factor H antibody (ABIN3023096, ABIN3023097, ABIN3023098 and ABIN6219387) at 1:1000 dilution.

Immunohistochemistry of paraffin-embedded human liver cancer using Complement factor H Rabbit pAb (ABIN3023096, ABIN3023097, ABIN3023098 and ABIN6219387) at dilution of 1:100 (40x lens).

IHC

Immunofluorescence analysis of HepG2 cells using Complement factor H Rabbit pAb (ABIN3023096, ABIN3023097, ABIN3023098 and ABIN6219387) at dilution of 1:100 (40x lens).

8 images

(1 reference)

(1 validation)

产品编号 ABIN3023097

发货至: 中国

Datasheet as PDF

抗原 See all Complement Factor H (CFH) 抗体

Complement Factor H (CFH)
抗原表位
15

9

5

3

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 20-270
适用
66

25

22

1

1

1

1

1

1
人
宿主
57

16

4

1
兔
Compatible Secondaries
克隆类型
54

24
多克隆
标记
37

9

8

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1
This Complement Factor H antibody is un-conjugated
应用范围
62

24

15

15

13

13

12

11

8

6

4

3

3

2

2

1

1

1
Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

序列

DCNELPPRRN TEILTGSWSD QTYPEGTQAI YKCRPGYRSL GNVIMVCRKG EWVALNPLRK CQKRPCGHPG DTPFGTFTLT GGNVFEYGVK AVYTCNEGYQ LLGEINYREC DTDGWTNDIP ICEVVKCLPV TAPENGKIVS SAMEPDREYH FGQAVRFVCN SGYKIEGDEE MHCSDDGFWS KEKPKCVEIS CKSPDVINGS PISQKIIYKE NERFQYKCNM GYEYSERGDA VCTESGWRPL PSCEEKSCDN P

交叉反应

人, 小鼠, 大鼠

产品特性

Polyclonal Antibodies

纯化方法

Affinity purification

免疫原

Recombinant fusion protein containing a sequence corresponding to amino acids 20-270 of human Complement factor H (NP_001014975.1).

亚型

IgG
anti-Complement Factor H (CFH) (AA 915-1156) antibody
CFH 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Complement Factor H (CFH) (Internal Region) antibody
Verified CFH 适用: 人 WB, IHC, ELISA, FACS 宿主: 山羊 Polyclonal unconjugated

anti-Complement Factor H (CFH) (AA 860-1231) antibody
CFH 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Complement Factor H (CFH) (AA 975-1217) antibody
CFH 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Complement Factor H (CFH) (AA 751-780) antibody
CFH 适用: 人 WB, IF, IHC (p) 宿主: 兔 Polyclonal RB18698 unconjugated

anti-Complement Factor H (CFH) (N-Term) antibody
CFH 适用: 人, 大鼠, 小鼠 WB, IHC, ELISA, ICC, IF 宿主: 兔 Polyclonal unconjugated

anti-Complement Factor H (CFH) (AA 20-270) antibody
CFH 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated

anti-Complement Factor H (CFH) (AA 1-449) antibody
CFH 适用: 人 WB 宿主: 兔 Polyclonal unconjugated

anti-Complement Factor H (CFH) (AA 751-780), (Middle Region) antibody
CFH 适用: 人 WB, IF, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated

anti-Complement Factor H (CFH) (AA 975-1217) antibody
CFH 适用: 大鼠 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C1 unconjugated

应用备注

WB,1:500 - 1:2000,IHC,1:50 - 1:200,IF,1:50 - 1:200

限制

仅限研究用
生效 #101403 (Immunofluorescence)

by

Alexander Lab, Jacobs School of Medicine and Biomedical Sciences, Department of Medicine, University of Buffalo

No.

#101403

日期

2017.07.19

抗原

Commplement Factor H

Lot Number

4390101

Method validated

Immunofluorescence

Positive Control

MRL/lpr lupus mice (14 weeks of age)

Negative Control

FH knockout MRL/lpr lupus mice

Notes

Passed. The complement factor H antibody ABIN3023097 works very well on murine kidney samples and shows the expected CFH localization and strong staining.

Validation Images

IF staining with ABIN3023097 of kidneys harvested from 14 week old MRL/lpr mice sufficient and deficient in factor H. Cryosections were fixed, stained and images acquired as described in the protocol.
Full Methods
Primary Antibody

ABIN3023097

Secondary Antibody

goat anti-rabbit rhodamine conjugated antibody (ThermoFisher Scientific, T-2769, lot 6251)

Full Protocol

Prepare kidneys of wt and complement factor H ko MRL/lpr lupus mice of 14 weeks of age.
Snap-freeze kidneys in 2-methylbutane cooled on dry ice and keep at −80°C until use.
Sections were cut at 8µm using the cryostat.
Dry sections for 30min at RT.
Hydrate sections in PBS 2x for 15min at RT.
Fix cryostat sections with 4% paraformaldehyde (PFA) for 15min at RT.
Wash sections 2x with PBS for 15min at RT.
Incubate sections with primary rabbit anti-Complement Factor H antibody (antibodies-online, ABIN3023097, 0004390101) diluted 1:100 in PBS ON at 4°C.
Wash sections 2x for 15min with PBS.
Incubate sections with secondary goat anti-rabbit rhodamine conjugated antibody (ThermoFisher Scientific, T-2769, lot 6251) diluted 1:100 in PBS for 30min at RT.
Wash sections 2x for 15min with PBS.
Incubate sections with ActinGreen 488 ReadyProbes Reagent (ThermoFisher Scientific, R37122, lot 1700569) diluted 1:100 in PBS for 30min at RT.
Wash sections 2x for 15min with PBS.
Image acquisition on a Zeiss microscope (Carl Zeiss, Oberkochen, Germany).

Experimental Notes
状态

Liquid

缓冲液

PBS with 0.02 % sodium azide,50 % glycerol, pH 7.3.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Avoid freeze / thaw cycles

储存条件

-20 °C

储存方法

Store at -20°C. Avoid freeze / thaw cycles.
Cheng, Malachi, Cipi, Ma, Brush, Agbaga, Punzo: "HK2 Mediated Glycolytic Metabolism in Mouse Photoreceptors Is Not Required to Cause Late Stage Age-Related Macular Degeneration-Like Pathologies." in: Biomolecules, Vol. 11, Issue 6, (2021) (PubMed).
抗原

Complement Factor H (CFH)

别名

CFH (CFH 产品)

别名

AHUS1 antibody, AMBP1 antibody, ARMD4 antibody, ARMS1 antibody, CFHL3 antibody, FH antibody, FHL1 antibody, HF antibody, HF1 antibody, HF2 antibody, HUS antibody, Mud-1 antibody, NOM antibody, Sas-1 antibody, Sas1 antibody, Fh antibody, CFH antibody, im:7147412 antibody, si:ch211-207o17.2 antibody, fH antibody, armd4 antibody, arms1 antibody, cfh antibody, cfhl3 antibody, fhl1 antibody, hf1 antibody, hf2 antibody, hus antibody, complement factor H antibody, complement component factor h antibody, complement factor H-related protein 5 antibody, complement factor H L homeolog antibody, CFH antibody, Cfh antibody, cfh antibody, LOC100399888 antibody, LOC100733902 antibody, cfh.L antibody

背景

This gene is a member of the Regulator of Complement Activation (RCA) gene cluster and encodes a protein with twenty short consensus repeat (SCR) domains. This protein is secreted into the bloodstream and has an essential role in the regulation of complement activation, restricting this innate defense mechanism to microbial infections. Mutations in this gene have been associated with hemolytic-uremic syndrome (HUS) and chronic hypocomplementemic nephropathy. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.,CFH,AHUS1,AMBP1,ARMD4,ARMS1,CFHL3,FH,FHL1,HF,HF1,HF2,HUS,Immunology & Inflammation,Cell Intrinsic Innate Immunity Signaling Pathway,CFH

分子量

51 kDa/139 kDa

基因ID

3075

UniProt

P08603

途径

Complement System, Cellular Response to Molecule of Bacterial Origin