Occludin 抗体 (AA 431-522)
(5 references)
(1 validation)-
- 抗原 See all Occludin (OCLN) 抗体
- Occludin (OCLN)
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抗原表位
- AA 431-522
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适用
- 人, 小鼠, Pig
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Occludin antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 交叉反应
- 人, 小鼠, Pig
- 预测反应
- Rat,Dog,Cow,Horse,Guinea Pig
- 纯化方法
- Purified by Protein A.
- 免疫原
- KLH conjugated synthetic peptide derived from mouse Occludin
- 亚型
- IgG
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anti-Occludin (OCLN) (C-Term) antibody
OCLN 适用: 人 WB, IHC (p), IHC (fro) 宿主: 山羊 Polyclonal unconjugated
anti-Occludin (OCLN) (AA 282-415) antibodyOCLN 适用: 人 WB, IHC (p) 宿主: 小鼠 Monoclonal CL1555 unconjugated
anti-Occludin (OCLN) (AA 423-522) antibodyOCLN 适用: 人 WB, ELISA, IP, IF 宿主: 小鼠 Monoclonal 1G7 unconjugated
anti-Occludin (OCLN) (AA 1-522) antibodyOCLN 适用: 人 WB, PLA 宿主: 兔 Polyclonal unconjugated
anti-Occludin (OCLN) (AA 1-522) antibodyOCLN 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Occludin (OCLN) (AA 282-415) antibodyOCLN 适用: 人 WB, IHC (f) 宿主: 小鼠 Monoclonal OCLN-2181 unconjugated
anti-Occludin (OCLN) (AA 285-430) antibodyOCLN 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Occludin (OCLN) (C-Term) antibodyOCLN 适用: 人, 小鼠, 大鼠, 犬 WB, ELISA, IHC, IF, IHC (p) 宿主: 小鼠 Monoclonal OC-3F10 unconjugated
anti-Occludin (OCLN) (AA 350-450) antibodyOCLN 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Occludin (OCLN) (AA 282-415) antibodyOCLN 适用: 人 IHC 宿主: 小鼠 Monoclonal MSVA-415M unconjugated
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- 应用备注
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WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IF(IHC-P) 1:50-200
IF(ICC) 1:50-200 - 限制
- 仅限研究用
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- by
- Confocal Imaging Core, Beth Israel Deaconess Medical Center
- No.
- #029632
- 日期
- 2014.03.23
- 抗原
- Lot Number
- 990698W
- Method validated
- Immunofluorescence
- Positive Control
- Mouse stomach tissue
- Negative Control
- Human fat tissue
- Notes
- Signal was detected in positive control sample and not in negative control sample.
- Primary Antibody
- Antibody: Occludin (OCLN)
- Catalog number: ABIN687337
- Lot number: 990698W
- Secondary Antibody
- Antibody: Donkey anti-Rabbit IgG (Heavy & Light Chain) Antibody (Alexa 647)
- Full Protocol
- Paraffin embedded positive and negative control tissue sections were deparaffinized and undergone antigen retrieval using 10 mM Sodium Citrate pH6 in a pressure cooker for 10 min.
- The tissue sections were incubated with 1 mg/mL Sodium borohydride for 10 min at room temperature to block autofluorescent background signal. The sections were then rinsed three times in TBS for 5 min each at RT.
- Tissue sections were blocked in 1 X TBS / 5% normal donkey serum for 60 min at RT.
- Tissue sections were incubated with primary antibody diluted 1:200 in 1X PBS / 5% normal donkey serum overnight at 4°C.
- Tissue sections were rinsed three times in TBS for 5 min each at RT.
- Cells were incubated with secondary antibody diluted 1:200 in 1X PBS / 5% normal donkey serum for 60 min at RT in dark.
- Tissue sections were rinsed three times in TBS for 5 min each at RT.
- Coverslips were mounted on slides with Prolong Gold anti-fade mounting media (Invitrogen)
- IF stained tissue sections were imaged with a Zeiss LSM 510 Meta confocal microscope.
- Experimental Notes
- No challenges notes
- OCLN is recommended to staining of mouse stomach tissue according to this protocol.
生效 #029632 (Immunofluorescence)!['独立验证'标志]( "成功验证")
!['独立验证'标志]( "成功验证")
Validation Images![Figure 1: Immunostaining (green) of mouse stomach tissue (A-C) and human fat tissue (D-F) with anti-OCLN (A, D), isotype control antibody (B, E), or with secondary alone (C, F). Tissues were counterstained with DAPI (blue).]()
Figure 1: Immunostaining (green) of mouse stomach tissue (A-C) and human fat tissue (D-F) with anti-OCLN (A, D), isotype control antibody (B, E), or with secondary alone (C, F). Tissues were counterstained with DAPI (blue).
Full Methods -
- 状态
- Liquid
- 浓度
- 1 μg/μL
- 缓冲液
- 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
- 储存液
- ProClin
- 注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
- 有效期
- 12 months
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: "Effects of insulin combined with idebenone on blood-brain barrier permeability in diabetic rats." in: Journal of neuroscience research, Vol. 93, Issue 4, pp. 666-77, (2015) (PubMed).
: "?-Conglycinin reduces the tight junction occludin and ZO-1 expression in IPEC-J2." in: International journal of molecular sciences, Vol. 15, Issue 2, pp. 1915-26, (2014) (PubMed).
: "Effects of soybean agglutinin on mechanical barrier function and tight junction protein expression in intestinal epithelial cells from piglets." in: International journal of molecular sciences, Vol. 14, Issue 11, pp. 21689-704, (2013) (PubMed).
: "Effects of soybean agglutinin on intestinal barrier permeability and tight junction protein expression in weaned piglets." in: International journal of molecular sciences, Vol. 12, Issue 12, pp. 8502-12, (2012) (PubMed).
: "Calcium-activated potassium channel activator down-regulated the expression of tight junction protein in brain tumor model in rats." in: Neuroscience letters, Vol. 493, Issue 3, pp. 140-4, (2011) (PubMed).
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: "Effects of insulin combined with idebenone on blood-brain barrier permeability in diabetic rats." in: Journal of neuroscience research, Vol. 93, Issue 4, pp. 666-77, (2015) (PubMed).
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- 抗原
- Occludin (OCLN)
- 别名
- Occludin (OCLN 产品)
- 别名
- AI503564 antibody, Ocl antibody, ocln antibody, oclnb antibody, tpmt antibody, OCLN antibody, wu:fd23h10 antibody, wu:fi13c01 antibody, zgc:113992 antibody, zgc:56359 antibody, BLCPMG antibody, occludin antibody, occludin S homeolog antibody, occludin a antibody, Ocln antibody, OCLN antibody, ocln.S antibody, ocln antibody, oclna antibody
- 背景
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Synonyms: Ocl, AI53564, Occludin, Ocln
Background: May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier.
- 基因ID
- 18260
- UniProt
- Q61146
- 途径
- Cell-Cell Junction Organization, Hepatitis C
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