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CD4 抗体 (AA 385-457)

Name: CD4 抗体 (AA 385-457)
SKU: ABIN671376
Availability: OutOfStock
Rating: 5 (1 reviews)

CD4 适用: 人, 小鼠, 大鼠, 小鸡, Pig FACS, WB, ELISA, IHC (fro), IHC (p), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated

Immunohistochemistry validation image for anti-CD4 (CD4) (AA 385-457) antibody (ABIN671376)

IHC

Mouse spleen cells probed with Rabbit Anti-CD4 Polyclonal Antibody, PE Conjugated for 30 minutes at 1:50.

11 images

(4 references)

(1 validation)

产品编号 ABIN671376

发货至: 中国

Datasheet as PDF

抗原 See all CD4 抗体

CD4
抗原表位
31

16

16

16

15

9

7

6

5

4

4

3

3

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 385-457
适用
354

198

112

37

23

20

19

18

15

11

10

10

8

7

6

6

6

6

6

2

2

1

1

1

1
人, 小鼠, 大鼠, 小鸡, Pig
宿主
328

145

108

4

2

1

1
兔
Compatible Secondaries
克隆类型
445

137

3
多克隆
标记
194

65

54

46

30

12

11

10

9

9

8

8

8

7

5

5

5

5

5

5

5

5

5

5

4

4

3

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1
This CD4 antibody is un-conjugated
应用范围
469

192

99

93

80

75

71

41

38

37

32

24

10

8

7

7

6

6

6

5

5

3

3

2

2

2

1

1

1

1

1
Flow Cytometry (FACS), Western Blotting (WB), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

交叉反应

小鸡, 人, 小鼠, Pig, 大鼠

预测反应

Dog,Cow,Sheep,Guinea Pig

纯化方法

Purified by Protein A.

免疫原

KLH conjugated synthetic peptide derived from Human CD4 (cytoplasmic domain)

亚型

IgG
anti-CD4 (CD4) antibody
CD4 适用: 人, 小鼠, 大鼠, 小鸡, 猴, Pig, 犬, Cat, 兔, Cow, 绵羊, 豚鼠, 山羊, 马, Hamster, 驴, Avian WB 宿主: 山羊 Polyclonal unconjugated

anti-CD4 (CD4) (AA 231-330) antibody
CD4 适用: 人, 小鼠, 大鼠 FACS, WB, ELISA, IHC (fro), IHC (p), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated

anti-CD4 (CD4) (N-Term) antibody
CD4 适用: 人 FACS, WB, CyTOF 宿主: 小鼠 Monoclonal MEM-241 unconjugated

anti-CD4 (CD4) (AA 300-400) antibody
CD4 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated

anti-CD4 (CD4) (AA 301-400) antibody
CD4 适用: 人, 小鼠, 大鼠 FACS, WB, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-CD4 (CD4) antibody
CD4 适用: 小鼠 FACS, IP, IHC (fro), ICC, Func 宿主: 大鼠 Monoclonal GK1-5 unconjugated

anti-CD4 (CD4) (AA 58-86) antibody
CD4 适用: 人 FACS, WB 宿主: 兔 Polyclonal unconjugated

anti-CD4 (CD4) (N-Term) antibody (FITC)
CD4 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-241 FITC

anti-CD4 (CD4) (N-Term) antibody (PE)
CD4 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-241 PE

anti-CD4 (CD4) antibody (PE)
CD4 适用: 大鼠 FACS 宿主: 小鼠 Monoclonal OX-35 PE

应用备注

WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

限制

仅限研究用
生效 #029634 (Immunohistochemistry)

by

Confocal Imaging Core, Beth Israel Deaconess Medical Center

No.

#029634

日期

2014.03.26

抗原

Lot Number

130703

Method validated

Immunohistochemistry

Positive Control

Mouse spleen tissue

Negative Control

Mouse muscle tissue

Notes

Signal was detected in positive control sample and not in negative control sample.

Validation Images

Figure 1: Mouse spleen stained with anti-CD4 (brown) and counterstained with hematoxylin.

Figure 2: Mouse muscle stained with anti-CD4 (brown) and counterstained with hematoxylin.

Figure 3: Mouse spleen stained with isotype control antibody (brown) and counterstained with hematoxylin.

Figure 4: Mouse spleen stained with secondary antibody only (brown) and counterstained with hematoxylin.
Full Methods
Primary Antibody

Antibody: CD4 Molecule (CD4)
Catalog number: ABIN671376
Lot number: 130703

Secondary Antibody

Antibody: Donkey anti-Rabbit IgG Antibody (Biotinylated)

Full Protocol

Immunohistochemistry was performed manually.
Sections were deparaffinized and rehydrated.
Sections were heated to 98°C for 10 min in 0.1M Borate buffer with 1 mM NaCl and 1 mM EDTA pH 8.0 for antigen retrieval.
Sections were incubated in 3% hydrogen peroxide for 10 min at room temperature to block endogenous peroxidases.
Sections were washed x 3 in Tris buffered saline (TBS).
Sections were blocked in 5 % normal donkey serum for 60 min at room temperature.
Sections were incubated with primary antibody diluted 1:200 in 5% normal donkey serum in TBS. Incubated at 4°C temperature overnight.
Sections were washed x 3 in Tris buffered saline.
Sections were incubated with secondary antibody diluted 1:400 in 5% normal donkey serum in TBS. Incubated for 60 min at room temperature..
Sections were washed x 3 in Tris buffered saline.
Sections were incubated with Vectastain ABC kit (Vector Lab PK6100) to enhance signal.
Sections were incubated with DAB chromogenic substrate (Vector Lab SK-4105) for 60 sec at RT.
Sections were washed x 3 in Distilled Water.
Sections were counterstained with hematoxylin for 20 sec.
Sections were washed x 1 in Distilled Water.
Sections were dehydrated, mounted and photographed under a Zeiss AxioImager M1 light microscope.

Experimental Notes

- No experimental challenges noted.
状态

Liquid

浓度

1 μg/μL

缓冲液

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

有效期

12 months
Jaime-Ramirez, Yu, Caserta, Yoo, Zhang, Lee, Hofmeister, Lee, Kumar, Pan, Kumar, Baiocchi, Teknos, Pichiorri, Kaur, Old: "Reolysin and Histone Deacetylase Inhibition in the Treatment of Head and Neck Squamous Cell Carcinoma." in: Molecular therapy oncolytics, Vol. 5, pp. 87-96, (2017) (PubMed).

Zhang, Fu, Li, Wang, Zhang, Liu: "Bone morphogenetic protein 7 is associated with the nodal invasion of colon cancer." in: Oncology letters, Vol. 11, Issue 3, pp. 1707-1712, (2016) (PubMed).

Smit, Bester, van den Heever, Schlegel, Botes, Dohmen: "Does prolonged post-mortem cold ischemic harvesting time influence cryopreserved pulmonary homograft tissue integrity?" in: Cell and tissue banking, (2015) (PubMed).
抗原

CD4

别名

CD4 (CD4 产品)

别名

cd4 antibody, L3T4 antibody, Ly-4 antibody, CD4mut antibody, W3/25 antibody, p55 antibody, CD4 molecule antibody, carcinoembryonic antigen-related cell adhesion molecule 5 antibody, T-cell surface glycoprotein CD4 antibody, CD4 antigen antibody, Cd4 molecule antibody, CD4 antibody, LOC100125525 antibody, cd4 antibody, Cd4 antibody

背景

Synonyms: T-cell surface glycoprotein CD4, Cd4, T-cell surface antigen T4/Leu-3, W3/25 antigen
Background: Integral membrane glycoprotein that plays an essential role in the immune response and serves multiple functions in responses against both external and internal offenses. In T-cells, functions primarily as a coreceptor for MHC class II molecule:peptide complex. The antigens presented by class II peptides are derived from extracellular proteins while class I peptides are derived from cytosolic proteins. Interacts simultaneously with the T-cell receptor (TCR) and the MHC class II presented by antigen presenting cells (APCs). In turn, recruits the Src kinase LCK to the vicinity of the TCR-CD3 complex. LCK then initiates different intracellular signaling pathways by phosphorylating various substrates ultimately leading to lymphokine production, motility, adhesion and activation of T-helper cells. In other cells such as macrophages or NK cells, plays a role in differentiation/activation, cytokine expression and cell migration in a TCR/LCK-independent pathway. Participates in the development of T-helper cells in the thymus and triggers the differentiation of monocytes into functional mature macrophages.

基因ID

24932

UniProt

P01730

途径

TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events