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E-cadherin 抗体 (AA 401-500)

Name: E-cadherin 抗体 (AA 401-500)
SKU: ABIN1387847
Availability: OutOfStock
Rating: 5 (1 reviews)

There are 14+ publications for this product available. The 兔多克隆 anti-E-cadherin antibody is suitable to detect E-cadherin in samples from 人, 小鼠和大鼠. It has been validated for WB, FACS, ELISA, ICC, IHC (p), IF (p), IHC (fro) 和 IF (cc). The antibody has been independently validated.

Flow Cytometry validation image for anti-Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) (AA 401-500) antibody (ABIN1387847) (E-cadherin 抗体 (AA 401-500))

FACS

Human MCF-7 lysates probed with Anti-E cadherin Polyclonal Antibody, Unconjugated at 1:5000 90min in 37˚C (E-cadherin 抗体 (AA 401-500))

Formalin-fixed and paraffin-embedded mouse intestine labeled with Anti-E cadherin/CD324 Polyclonal Antibody, Unconjugated (ABIN1387847) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated used at 1:200 dilution for 40 minutes at 37°C. (E-cadherin 抗体 (AA 401-500))

Formalin-fixed and paraffin embedded human lung carcinoma labeled with Rabbit Anti E cadherin/CD324 Polyclonal Antibody, Unconjugated (ABIN1387847) at 1:200 followed by conjugation to the secondary antibody and DAB staining (E-cadherin 抗体 (AA 401-500))

14 images

(14 references)

(1 validation)

产品编号 ABIN1387847

发货至: 中国

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Quick Overview for E-cadherin 抗体 (AA 401-500) (ABIN1387847)

抗原

See all E-cadherin (CDH1) 抗体

E-cadherin (CDH1) (Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1))

适用

人, 小鼠, 大鼠

宿主

兔

克隆类型

多克隆

标记

This E-cadherin antibody is un-conjugated

应用范围

Western Blotting (WB), Flow Cytometry (FACS), ELISA, Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (Cultured Cells) (IF (cc))

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AA 401-500

交叉反应

人, 小鼠, 大鼠

预测反应

Cow,Pig,Horse,Rabbit

纯化方法

Purified by Protein A.

免疫原

KLH conjugated synthetic peptide derived from human E-cadherin

亚型

IgG
anti-Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) antibody
KO Validated CDH1 适用: 人 WB, IF, IP, ICC, IHC (p), IHC (wm) 宿主: 兔 Polyclonal unconjugated

anti-Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) (C-Term) antibody
CDH1 适用: 人, 小鼠, 大鼠 WB, IHC, IF, ELISA, ICC 宿主: 兔 Polyclonal unconjugated

anti-Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) (AA 381-480) antibody
CDH1 适用: 人 WB, ELISA, IHC (p), PLA 宿主: 小鼠 Monoclonal 3F4 unconjugated

anti-Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) antibody
CDH1 适用: 人, 小鼠, 大鼠, Pig, 猴, 小鸡, 犬, 兔, Cow, 马, 豚鼠, Avian, Cat, 驴, 山羊, Hamster, 绵羊 WB, IF, IHC (p), IHC (fro) 宿主: 山羊 Polyclonal unconjugated

anti-Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) antibody
CDH1 适用: 人, 小鼠, 大鼠, Pig, 猴, 小鸡, 犬, 兔, Cow, 马, 豚鼠, Avian, Cat, 驴, 山羊, Hamster, 绵羊 WB, IF, IHC (p), IHC (fro) 宿主: 山羊 Polyclonal unconjugated

anti-Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) (AA 810-890) antibody
CDH1 适用: 人, 小鼠, 大鼠 WB, IF, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) antibody
CDH1 适用: 人 WB, IHC, IF, StM, ISt 宿主: 小鼠 Monoclonal CDH1-1525 unconjugated

anti-Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) (AA 375-631) antibody
CDH1 适用: Pig WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) (AA 700-800) antibody
CDH1 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) (AA 155-707) antibody
CDH1 适用: 人 WB, FACS, IF, ELISA 宿主: 小鼠 Monoclonal 9H2D3 unconjugated

应用备注

WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

限制

仅限研究用
生效 #029768 (Flow Cytometry)

by

Flow Cytometry & Cell Separation Facility, Purdue University

No.

#029768

日期

2014.07.22

抗原

Lot Number

130902

Method validated

Flow Cytometry

Positive Control

MCF-7 cells

Negative Control

SH-SY5Y cells

Notes

A weak but specific signal is observed in the positive control MCF7 cells stained with anti-E-Cadherin plus secondary antibody compared with isotype, secondary only and unstained cells. No staining was observed in the negative control SH-SY5Y cells as expected.

Validation Images

Figure 1: Positive control MCF7 cells. The red histogram is unstained cells, the blue histogram is cells stained with secondary antibody alone, the green histogram is cells stained with rabbit IgG isotype control antibody plus secondary antibody and the orange histogram is cells stained with anti-E-Cadherin plus secondary antibody. The secondary antibody is a goat anti-rabbit IgG-Alexa 647 (Jackson Immunoresearch).

Figure 2: Negative control SH-SY5Y cells. The red histogram is unstained cells, the blue histogram is cells stained with secondary antibody alone, the green histogram is cells stained with rabbit IgG isotype control antibody plus secondary antibody and the orange histogram is cells stained with anti-E-Cadherin plus secondary antibody. The secondary antibody is a goat anti-rabbit IgG-Alexa 647 (Jackson Immunoresearch).
Full Methods
Primary Antibody

Antigen: Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1)
Catalog number: ABIN1387847
Lot number: 130902
Dilution: 1 µg in 100 µL 1X PBS containing 0.5% BSA

Secondary Antibody

Antibody: Goat anti-rabbit IgG-Alexa 647
Dilution: 1:500 in 1X PBS containing 0.5% BSA

Full Protocol

Positive and negative control cells were cultured in DMEM + 10% FBS. - Positive and negative control cells were washed once with phosphate-buffered saline (PBS) and harvested with a non-enzymatic cell dissociation solution (Cellstripper, Mediatech, Inc).
Detached cells were washed twice and resuspended in 100 µL 1X PBS containing 0.5% BSA: - unstained cells - secondary antibody alone - isotype control antibody + secondary antibody - primary antibody + secondary antibody
Cells were incubated for 30 min on ice.
Labeled cells were washed twice in PBS containing 0.5% BSA.
Cells were resuspended with 1X PBS containing 0.5% BSA + 10% goat serum and incubated for 15 min at room temperature.
Goat anti-rabbit IgG-Alexa 647 secondary antibody (Jackson Immunoresearch) was added at a 1:500 dilution. The cells were incubated for 30 min in the dark on ice.
Labeled cells were washed twice in PBS containing 0.5% BSA.
Propidium Iodide (PI) was added to discern live cells from dead cells.
Cells were analyzed on a FACSAria III (BD Biosciences) using a red laser (640 nm excitation / 660 nm emission).

Experimental Notes

- The data displayed is gated on PI negative cells.
状态

Liquid

浓度

1 μg/μL

缓冲液

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

有效期

12 months
Guo, Gao, Sui, Jiao, Sun, Fu, Jin: "miR-375-3p/YWHAZ/β-catenin axis regulates migration, invasion, EMT in gastric cancer cells." in: Clinical and experimental pharmacology & physiology, Vol. 46, Issue 2, pp. 144-152, (2019) (PubMed).

Yun, Gao, Yue, Guo, Li, Sang: "Sulfate Aerosols Promote Lung Cancer Metastasis by Epigenetically Regulating the Epithelial-to-Mesenchymal Transition (EMT)." in: Environmental science & technology, Vol. 51, Issue 19, pp. 11401-11411, (2018) (PubMed).

Fang, Liu, Wu, Liu, Pan, Li: "Upregulation of long noncoding RNA CCAT1-L promotes epithelial-mesenchymal transition in gastric adenocarcinoma." in: OncoTargets and therapy, Vol. 11, pp. 5647-5655, (2018) (PubMed).

Lin, Zhang, Dai, Zhang, Zhang, Xue, Wu: "TFF3 Contributes to Epithelial-Mesenchymal Transition (EMT) in Papillary Thyroid Carcinoma Cells via the MAPK/ERK Signaling Pathway." in: Journal of Cancer, Vol. 9, Issue 23, pp. 4430-4439, (2018) (PubMed).

Wang, Nikhil, Viccaro, Chang, White, Shah: "Phosphorylation-dependent regulation of ALDH1A1 by Aurora kinase A: insights on their synergistic relationship in pancreatic cancer." in: BMC biology, Vol. 15, Issue 1, pp. 10, (2017) (PubMed).

Borin, Shankar, Angara, Rashid, Jain, Iskander, Ara, Lebedyeva, Korkaya, Achyut, Arbab: "HET0016 decreases lung metastasis from breast cancer in immune-competent mouse model." in: PLoS ONE, Vol. 12, Issue 6, pp. e0178830, (2017) (PubMed).

Liu, Xiao: "Notch1 signaling induces epithelial-mesenchymal transition in lens epithelium cells during hypoxia." in: BMC ophthalmology, Vol. 17, Issue 1, pp. 135, (2017) (PubMed).

Wang, Nikhil, Viccaro, Chang, Jacobsen, Sandusky, Shah: "The Aurora-A-Twist1 axis promotes highly aggressive phenotypes in pancreatic carcinoma." in: Journal of cell science, Vol. 130, Issue 6, pp. 1078-1093, (2017) (PubMed).

Short, Kasper, van der Aa, Andeweg, Zaaraoui-Boutahar, Goeijenbier, Richard, Herold, Becker, Scott, Limpens, Koster, Bárcena, Fouchier, Kirkpatrick, Kuiken: "Influenza virus damages the alveolar barrier by disrupting epithelial cell tight junctions." in: The European respiratory journal, Vol. 47, Issue 3, pp. 954-66, (2016) (PubMed).

Hu, Duan, Li, Wang, Wang, Chu, Sun, Liu, Wang, Lu, Wen: "Hydroxysafflor Yellow A Ameliorates Renal Fibrosis by Suppressing TGF-β1-Induced Epithelial-to-Mesenchymal Transition." in: PLoS ONE, Vol. 11, Issue 4, pp. e0153409, (2016) (PubMed).

Neelam, Brooks, Cammarata: "Lenticular cytoprotection, part 2: link between glycogen synthase kinase-3?, epithelial to mesenchymal transition, and mitochondrial depolarization." in: Molecular vision, Vol. 20, pp. 1758-75, (2015) (PubMed).

Jayachandran, Lugo, Heiling, Miller, Rule, Lieske: "Extracellular vesicles in urine of women with but not without kidney stones manifest patterns similar to men: a case control study." in: Biology of sex differences, Vol. 6, pp. 2, (2015) (PubMed).

Chen, Cheng, Chen, Sue, Liu, Cheng, Hsu, Chen: "MicroRNA-328 inhibits renal tubular cell epithelial-to-mesenchymal transition by targeting the CD44 in pressure-induced renal fibrosis." in: PLoS ONE, Vol. 9, Issue 6, pp. e99802, (2014) (PubMed).

Cai: "Roles of transcriptional factor Snail and adhesion factor E-cadherin in clear cell renal cell carcinoma." in: Experimental and therapeutic medicine, Vol. 6, Issue 6, pp. 1489-1493, (2013) (PubMed).
抗原

E-cadherin (CDH1) (Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1))

别名

E cadherin

背景

Synonyms: UVO, CDHE, ECAD, LCAM, Arc-1, CD324, Cadherin-1, CAM 12/8, Epithelial cadherin, E-cadherin, Uvomorulin, CDH1
Background: Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells, cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells. Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7. E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production.

基因ID

999

UniProt

P12830

途径

WNT signaling, Sensory Perception of Sound, Cell-Cell Junction Organization, Tube Formation