NFKB1 抗体 (AA 860-969)
(1 validation)-
- 抗原 See all NFKB1 抗体
- NFKB1 (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells 1 (NFKB1))
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抗原表位
- AA 860-969
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This NFKB1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Proximity Ligation Assay (PLA)
- 原理
- Mouse monoclonal antibody raised against a partial recombinant NFKB1.
- 序列
- MDNYEVSGGT VRELVEALRQ MGYTEAIEVI QAASSPVKTT SQAHSLPLSP ASTRQQIDEL RDSDSVCDSG VETSFRKLSF TESLTSGASL LTLNKMPHDY GQEGPLEGKI
- 交叉反应
- 人
- 产品特性
- Antibody Reactive Against Recombinant Protein.
- 免疫原
- NFKB1 (AAH51765, 860 a.a. ~ 969 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
- 克隆位点
- 4A11
- 亚型
- IgG1
- Top Product
- Discover our top product NFKB1 Primary Antibody
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anti-Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells 1 (NFKB1) (AA 740-964) antibody
NFKB1 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells 1 (NFKB1) (AA 1-961) antibodyNFKB1 适用: 人 WB, IHC, ELISA, IF, ChIP 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells 1 (NFKB1) (AA 860-969) antibodyNFKB1 适用: 人 WB, ELISA, IF, PLA, RNAi 宿主: 小鼠 Monoclonal 2E6 unconjugated
anti-Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells 1 (NFKB1) (C-Term) antibodyNFKB1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells 1 (NFKB1) (pSer927) antibodyNFKB1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells 1 (NFKB1) antibodyNFKB1 适用: 人 WB, IHC, ELISA, FACS, ICC 宿主: 小鼠 Monoclonal 5D10D11 unconjugated
anti-Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells 1 (NFKB1) (pSer893) antibodyNFKB1 适用: 人, 小鼠 ELISA, IHC (p), IF (cc), IF (p), FACS, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells 1 (NFKB1) (pSer337) antibodyNFKB1 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells 1 (NFKB1) (pSer337) antibodyNFKB1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells 1 (NFKB1) antibodyNFKB1 适用: 人 WB, IHC, ELISA, FACS 宿主: 小鼠 Monoclonal 5D10 unconjugated
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
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- by
- Klinik für Anästhesiologie, Intensivmedizin und Schmerztherapie des Universitätsklinikums Knappschaftskrankenhaus Bochum
- No.
- #104071
- 日期
- 2019.11.21
- 抗原
- NFKB1
- Lot Number
- 11067-4A11
- Method validated
- Immunofluorescence
- Positive Control
U-937 human monocytic cell line
- Negative Control
No primary antibody control
- Notes
Passed. ABIN561981 specifically stains NF-κB1 in U-937 human monocytic cells.
- Primary Antibody
- ABIN561981
- Secondary Antibody
- AF488-conjugated AffiniPure alpaca anti-mouse IgG antibody (Jackson Immuno Research, 615-545-214)
- Full Protocol
- Grow U-937 cells in RPMI 1640 Medium (Gibco, 21875034) supplemented with 10% FCS (Gibco, A3840001) and Pen/Strep (Gibco, 15140122), at 37°C and 5% CO2 in 300µL in 24-well plates (Sarstedt AG&Co. KG).
- Use cytospin to spin cells onto slide.
- Fix cells on coverslide in 4% paraformaldehyde (Carl Roth GmbH, 0335.1) for 30min at RT.
- Wash cells 3x for 5min with PBS (Sigma-Aldrich, 8537).
- Permeabilize cells in 100µL PBS containing 0.1 % Triton X-100 (Sigma-Aldrich, T8787) for 5min at RT.
- Wash cells 3x for 5min with PBS.
- Block non-specific binding with 1x Duolink Blocking solution (Sigma-Aldrich, DUO82007) for 30min at RT.
- Incubate cells with primary mouse anti-human NF-κB1/p50 antibody (antibodies-online, ABIN561981, lot 11067-4A11) diluted 1:50 in 1x Duolink Antibody diluent (Sigma-Aldrich, DUO82008) ON at 4°C.
- Wash cells 3x for 5min with PBS.
- Incubate cells with secondary AF488-conjugated AffiniPure alpaca anti-mouse IgG antibody (Jackson Immuno Research, 615-545-214) diluted 1:400 in 1x Duolink Antibody diluent for 1h at RT in the dark.
- Wash cells 2x for 5min with PBS.
- Mount coverslips on glass slides in 50µL ProLong Gold antifade mountant with DAPI (Invitrogen, P36931).
- Image acquisition with an Olympus widefield microscope.
- Experimental Notes
Strong green staining dependent on the presence of primary antibody indicates that the antibody works. Negative control lacking the primary antibody shows only background fluorescence.
生效 #104071 (Immunofluorescence)!['独立验证'标志]( "成功验证")
!['独立验证'标志]( "成功验证")
Validation Images![U937 monocytic cells stained with with anti-NF-κB1/p50 antibody ABIN561981 and an AF488-labeled secondary antibody (bottom row, green). Counterstain with DAPI (blue). Negative control without primary antibody (top row) shows no AF488 signal.]()
U937 monocytic cells stained with with anti-NF-κB1/p50 antibody ABIN561981 and an AF488-labeled secondary antibody (bottom row, green). Counterstain with DAPI (blue). Negative control without primary antibody (top row) shows no AF488 signal.
Full Methods -
- 缓冲液
- In 1x PBS, pH 7.4
- 注意事项
- Aliquot to avoid repeated freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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- 抗原
- NFKB1 (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells 1 (NFKB1))
- 别名
- NFKB1 (NFKB1 产品)
- 别名
- GKLP antibody, NKTL antibody, NTKL antibody, P105 antibody, TAPK antibody, TEIF antibody, TRAP antibody, EBP-1 antibody, KBF1 antibody, NF-kB1 antibody, NF-kappa-B antibody, NF-kappaB antibody, NFKB-p105 antibody, NFKB-p50 antibody, NFkappaB antibody, p105 antibody, p50 antibody, NF-kB antibody, NF-KB1 antibody, ebp-1 antibody, kbf1 antibody, nf-kappa-b antibody, nf-kappaB antibody, nfkb-p105 antibody, nfkb-p50 antibody, NF-kappaB1 antibody, p50/p105 antibody, BICDR-1 antibody, bicdr1 antibody, ccdc64 antibody, SCY1 like pseudokinase 1 antibody, nuclear factor kappa B subunit 1 antibody, nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 L homeolog antibody, nuclear factor NF-kappa-B p105 subunit antibody, nucleoporin 107 antibody, nuclear factor of kappa light polypeptide gene enhancer in B cells 1, p105 antibody, nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 antibody, SCYL1 antibody, NFKB1 antibody, Nfkb1 antibody, nfkb1.L antibody, CpipJ_CPIJ012236 antibody, Nup107 antibody, nfkb1 antibody
- 背景
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Full Gene Name: nuclear factor of kappa light polypeptide gene enhancer in B-cells 1
Synonyms: DKFZp686C01211,EBP-1,KBF1,MGC54151,NF-kappa-B,NFKB-p105,NFKB-p50,p105,p50 - 基因ID
- 4790
- 途径
- p53 Pathway, NF-kappaB Signaling, RTK signaling, TCR Signaling, TLR signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Myometrial Relaxation and Contraction, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Toll-Like Receptors Cascades, BCR Signaling, S100 Proteins
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