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RFP 抗体 (Biotin)

RFP 适用: Discosoma WB, ELISA 宿主: 小鸡 Polyclonal Biotin
产品编号 ABIN1607901
发货至: 中国
  • 抗原 See all RFP 抗体
    RFP (Red Fluorescent Protein (RFP))
    适用
    • 51
    • 1
    Discosoma
    宿主
    • 23
    • 14
    • 9
    • 5
    • 2
    小鸡
    克隆类型
    • 31
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    多克隆
    标记
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    This RFP antibody is conjugated to Biotin
    应用范围
    • 27
    • 22
    • 14
    • 12
    • 10
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    • 6
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    • 2
    • 1
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    Western Blotting (WB), ELISA
    原理
    Anti-RFP is designed to detect recombinant RFP.
    纯化方法
    RFP Antibody was prepared from egg yolks by a multi-step process which includes filtration, delipidation, salt fractionation and extensive dialysis against the buffer stated above. RFP Antibody was tested by ELISA, western blot and immunoelectrophoresis.
    免疫原
    The immunogen is a Red Fluorescent Protein (RFP) fusion protein corresponding to the full length amino acid sequence (234aa) derived from the mushroom polyp coral Discosoma.
    Immunogen Type: Recombinant Protein
    Featured
    Discover our best selling RFP Primary Antibody
  • 应用备注
    Anti-RFP is designed to detect recombinant RFP. This antibody can be used to detect RFP by ELISA (sandwich or capture) for the direct binding of antigen. For immunoblotting use either alkaline phosphatase or peroxidase conjugated polyclonal anti-RFP to detect RFP or RFP containing proteins on western blots. Optimal titers for applications should be determined by the researcher. This product shows optimal performance by western blot.
    ELISA Dilution: 1:10.000
    Western Blot Dilution: 1:1.000 - 1:3.000
    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式
    Restore with 100 µL deionized water (or equivalent)
    浓度
    1 mg/mL
    缓冲液
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
    储存条件
    4 °C/-20 °C
    储存方法
    Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use.
    有效期
    Expiration date is one (1) year from date of opening.
  • Jo, Xu, Deniz, Cherian, DeVries, Zhu: "Intersectional Strategies for Targeting Amacrine and Ganglion Cell Types in the Mouse Retina." in: Frontiers in neural circuits, Vol. 12, pp. 66, (2019) (PubMed).

  • 抗原
    RFP (Red Fluorescent Protein (RFP))
    Abstract
    RFP 产品
    背景
    Antibodies to RFP (Discosoma spp.) are intended for use in immunological assays including ELISA, western blotting, fluorometry and fluorescence activated cell sorting (FACS). Synonyms: DsRed, rDsRed, Discosoma sp. Red Fluorescent Protein, Red fluorescent protein drFP583
    基因ID
    55976617
    UniProt
    Q9U6Y8
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