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CHEK1 抗体 (Ser280)

Name: CHEK1 抗体 (Ser280)
Brand: antibodies-online
SKU: ABIN1574028
Availability: OutOfStock

CHEK1 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated

Western blot analysis of cell lysate using 1 µg/mL Rabbit Anti-Chk1 (Ser280) Polyclonal Antibody (ABIN398709) Lane 1: Hela cell lysateLane 2: HEK293 cell lysateThe signal was developed with IRDyeTM 800 Conjugated Goat Anti-Rabbit IgG. (CHEK1 抗体 (Ser280))

1 image

产品编号 ABIN1574028

发货至: 中国

Datasheet as PDF

抗原 See all CHEK1 抗体

CHEK1 (Checkpoint Kinase 1 (CHEK1))
抗原表位
36

29

26

19

13

8

7

6

5

3

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
Ser280
适用
196

118

68

16

9

9

6

5

5

3

2

2

2

1

1
人
宿主
205

14

6

5
兔
Compatible Secondaries
克隆类型
206

25
多克隆
标记
123

13

9

9

9

9

9

8

8

8

4

4

4

4

4

3

3
This CHEK1 antibody is un-conjugated
应用范围
165

85

53

41

40

36

34

16

11

10

7

6

3

3

2

1

1
Western Blotting (WB), ELISA

特异性

Rabbit Anti-Chk1 (Ser280) Polyclonal Antibody detects endogenous levels of human Chk1 protein.

纯化方法

Immunoaffinity chromatography

免疫原

Synthetic peptide (KLH-coupled) derived from AA surrounding Ser280 of human Chk1

亚型

IgG

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anti-Checkpoint Kinase 1 (CHEK1) (pSer280) antibody
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anti-Checkpoint Kinase 1 (CHEK1) (pSer296) antibody
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anti-Checkpoint Kinase 1 (CHEK1) (pSer345) antibody
CHEK1 适用: 人 WB, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated

anti-Checkpoint Kinase 1 (CHEK1) (Middle Region) antibody
CHEK1 适用: 人, 小鼠, 大鼠, 犬, Cow, 马, 兔, 豚鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-Checkpoint Kinase 1 (CHEK1) (pSer280) antibody
CHEK1 适用: 人, 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Checkpoint Kinase 1 (CHEK1) (pSer317) antibody
CHEK1 适用: 小鼠, 小鸡 ELISA, IHC (p), ICC, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

应用备注

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

ELISA: 0.05-0.2 µg/mL Western blot: 0.5-2 µg/mL
Other applications: user-optimized

限制

仅限研究用
状态

Lyophilized

缓冲液

PBS, pH 7.4, containing 0.02 % sodium azide.

储存液

Sodium azide

注意事项

WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

储存条件

4 °C/-20 °C

储存方法

The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
抗原

CHEK1 (Checkpoint Kinase 1 (CHEK1))

别名

Chk1 (CHEK1 产品)

别名

An08g10320 antibody, AO090003000441 antibody, CHK1 antibody, chk1 antibody, C85740 antibody, Chk1 antibody, rad27 antibody, id:ibd2720 antibody, zgc:56093 antibody, CHEK1 antibody, checkpoint kinase 1 antibody, serine/threonine-protein kinase chk1 antibody, serine/threonine-protein kinase Chk1 antibody, CAMK/CAMKL/CHK1 protein kinase Chk1 antibody, checkpoint kinase 1 S homeolog antibody, CHEK1 antibody, ANI_1_2436074 antibody, AOR_1_768154 antibody, PTRG_04183 antibody, SJAG_01680 antibody, PAAG_04978 antibody, MCYG_03290 antibody, VDBG_03742 antibody, chek1.S antibody, Chek1 antibody, chek1 antibody

背景

Chk1 is a serine/threonine kinase first identified as a checkpoint kinase in Schizosaccharomyces pombe. Chk1 is an evolutionarily conserved protein kinase that functions to ensure genomic integrity upon genotoxic stress. Chk1 is activated through phosphorylation by ATR/ATM and plays an important role in DNA damage checkpoint control, embryonic development, and tumor suppression. The activation of Chk1 in response to DNA replication blockages and certain forms of genotoxic stress involves phosphorylation of Ser317 and Ser345. Chk1 is also phosphorylated at Ser280 and Ser296 following DNA damage. Activated Chk1 further activates downstream factors that mediate cell cycle arrest and other cell physiological events through phosphorylation. These key cell cycle factors include p53, Cdc25C and more. Rabbit Anti-Chk1 (Ser280) Polyclonal Antibody is developed in rabbit using a KLH-coupled synthetic peptide derived from the residues surrounding Ser 280 of human Chk1 (Swiss Prot: O14757).

途径

p53 Pathway, Apoptosis, Cell Division Cycle, DNA Damage Repair