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Unconjugated Antibodies

Unconjugated secondary antibodies refer to antibodies that are not directly labeled or attached to any reporter molecules, such as enzymes or fluorophores. They are typically raised against the species-specific immunoglobulins of the primary antibodies used in an experiment. They have some advantages over conjugated secondary antibodies, although this should always be considered depending on the experiment:

1. flexibility in fluorescent color selection:

A major advantage of using unconjugated secondary antibodies is the flexibility in choosing the fluorescent color for the experiments. Unlike conjugated secondary antibodies, which are already labeled with a specific dye or reporter molecule label, unconjugated secondary antibodies allow the researcher to conjugate the antibody as needed with a custom label that meets the specific requirements of the experimental setup. This flexibility is particularly important when different fluorescent colors are to be used, either to detect multiple proteins simultaneously or to label specific cell components or tissue structures.

2. reduction of background signals:

The use of unconjugated secondary antibodies can help reduce background signals in experiments. Antibodies can form non-specific bonds and thus contribute to increased background signal. By using unconjugated secondary antibodies of the same isotype, such problems can be minimized. This results in a higher signal-to-noise ratio and improved accuracy of results.

3. signal amplification

Unconjugated secondary antibodies can amplify the signal in an immunoassay by binding to primary antibodies that have already bound to the target antigen. Thus, they locally increase the concentration of Fc fragments to which labeled tertiary antibodies can bind. Since multiple non-conjugated secondary antibodies can bind to a single primary antibody, this leads to signal amplification and increased sensitivity of the respective immunoassay.

Below you will find our unconjugated secondary antibodies, which you can easily browse and filter by the properties relevant to you. If you have any questions, please contact our scientific customer support.

1,223 results :

ELISA, IHC, WB, CUT&Tag, CUT&RUN Polyclonal IgG unconjugated
Pubmed 105 references Independent Validation 1 validation
产品编号 ABIN101961
ELISA, WB, FACS, ICC Monoclonal EM-07 IgG1 unconjugated
产品编号 ABIN302085
产品编号 ABIN2862700
FACS, IHC (fro), WB Monoclonal IVA285-1 IgG1 unconjugated
Pubmed 1 reference
产品编号 ABIN94401
ELISA, IHC, WB Polyclonal IgG unconjugated Fab fragment
Pubmed 1 reference
产品编号 ABIN965357
ELISA, IHC, WB, LF Polyclonal IgG unconjugated
产品编号 ABIN102001
ELISA, IHC, WB Polyclonal IgG unconjugated
产品编号 ABIN102079
FACS, IHC (fro) Monoclonal IA6-2 IgG2a kappa unconjugated
Pubmed 6 references
产品编号 ABIN2749203
IHC Monoclonal MSVA-700R IgG Recombinant Antibody unconjugated
产品编号 ABIN7477497
IHC (p), WB, FACS, IF Monoclonal IM373 IgG1 kappa unconjugated
产品编号 ABIN3026664
  • 类型 Secondary
    • Secondary
  • 抗原
    • IgG
    • IgM
    • IgA
    • IgA, IgG, IgM
    • Ig
    • IgG1
    • IgE
    • IgY
    • IgG2
    • IgG2a
    • IgD
    • IgG3
    • IgG2b
    • kappa Light Chain
    • IgG2c
    • IgG4
    • Igk
    • IgG Fc
    • lambda
    • Ig lambda
    • IgG, IgM
    • Lambda-IgLC
    • IgG + IgA + IgM + IgD
    • IGJ
    • IgA1
    • kappa/lambda Light Chain (Kappa/Lambda-IgLC)
    • IgA Secretory Component
    • IgA1,2
    • IgA2
    • IgG1, IgG2a, IgG2b, IgG2c
    • IgG2ab
    • IGHM
    • CD79a (CD79A)
    • Ig Light Chains
    • IgG lambda
    • IgG1, IgG2a, IgG2b, IgG3
    • IgG2, IgG3
    • IgG2a(1a)
    • IgG2a(1b)
    • IgM Heavy Chain
    • IGHA2
    • IGHG1
    • Light Chain
    • lambda Chain
    • Bence-Jones Protein kappa
    • Bence-Jones Protein lambda
    • CD79
    • GLS2
    • Ig lambda C
    • IgA + IgD + IgE + IgG + IgM
    Please use search to find more...
  • 应用范围
    • ELISA
    • Western Blotting (WB)
    • Immunohistochemistry (IHC)
    • Immunoprecipitation (IP)
    • Immunocytochemistry (ICC)
    • Flow Cytometry (FACS)
    • Immunoelectrophoresis (IEP)
    • Immunofluorescence (IF)
    • Immunodiffusion (ID)
    • Enzyme Immunoassay (EIA)
    • Immunoassay (IA)
    • Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    • Immunohistochemistry (Frozen Sections) (IHC (fro))
    • Radial Immunodiffusion (RID)
    • Dot Blot (DB)
    • FLISA
    • Radioimmunoassay (RIA)
    • Lateral Flow (LF)
    • Ouchterlony Double Diffusion (ODD)
    • Immunofluorescence (fixed cells) (IF/ICC)
    • ELISpot
    • Functional Studies (Func)
    • Haemagglutination (H)
    • Immunochromatography (IC)
    • Standard (STD)
    • Chromatin Immunoprecipitation (ChIP)
    • Conjugation (Con)
    • Fluorescence Microscopy (FM)
    • Agglutination (AGG)
    • Cleavage Under Targets and Release Using Nuclease (CUT&RUN)
    • Cleavage Under Targets and Tagmentation (CUT&Tag)
    • ELISA (Detection)
    • Immunofluorescence (Cultured Cells) (IF (cc))
    • Immunofluorescence (Paraffin-embedded Sections) (IF (p))
    • Immunohistochemistry (Acetone-fixed) (IHC (af))
    • Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))
    • Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
    • Labeling (Lbl)
    • Protein Assay (PrA)
  • 适用
    • Human
    • Mouse
    • Rat
    • Rabbit
    • Goat
    • Chicken
    • Cow
    • Guinea Pig
    • Dog
    • Horse
    • Cat
    • Sheep
    • Monkey
    • Pig
    • Golden Syrian Hamster
    • Hamster
    • Donkey
    • Duck
    • Ferret
    • Llama
    • Armenian Hamster
    • Turkey
    • Rhesus Monkey
    • Camel
    • Chimpanzee
    • Deer
    • Fish
    • Mammalian
    • Pigeon
    • Reptile
    • Alligator
    • Atlantic Salmon
    • Avian
    • Baboon
    • Bat
    • Cynomolgus
    • Green Monkey
    • Hispid Cotton Rat
    • Iberian lynx (Lynx pardinus)
    • Mustelid
    • Opossum
    • Raccoon
    • Rainbow Trout
    • Salmon
    • Salmonella
    • Tree shrew
    • Tursiops truncatus
    • Wolf
    • Zebrafish (Danio rerio)
  • 论文引用
  • 图像
  • 独立验证
  • Preservative free only
  • Carrier free only
  • 抗体来源
    • Goat
    • Rabbit
    • Mouse
    • Sheep
    • Donkey
    • Rat
    • Pig
    • Chicken
    • Chicken egg
    • Guinea Pig
    • Alpaca
    • Cow
    • Llama
  • 克隆形成能力
    • Polyclonal
    • Monoclonal
  • 克隆
    • 11-44-2
    • 187-1
    • 1E1
    • 23G3
    • 4D2D9G8
    • 4F4cc
    • 8D6
    • A-1
    • B3506B4
    • DIG11-124-1
    • FIG10-102-8
    • G-1
    • IVA285-1
    • JC5-1
    • L-1
    • M 2A1
    • M-4
    • M2A1
    • Mix
    • RM110
    • SB73a
    • SB84a
    • 10A4
    • 11-26
    • 14A3
    • 14A4
    • 15D6
    • 16C7
    • 1A23
    • 1A8
    • 1F6
    • 22G1-H8
    • 2A 8F4
    • 2A8F4
    • 2A9
    • 2B 10A8
    • 2B10A8
    • 2C 8F1
    • 2C1
    • 2C8F1
    • 2D7-A9-C8
    • 2E4
    • 3D8-H7-D4
    • 3E12-H4-G4
    • 3G2
    • 3H10G5F2
    • 4-00E-003
    • 48
    • 4A10
    • 4A11
    Please use search to find more...
  • 抗原表位
    • Heavy & Light Chain
    • Fc Region
    • Heavy Chain
    • F(ab')2 Region
    • Fab Region
    • Chain gamma
    • Light Chain
    • Chain kappa
    • Free & Bound
    • Chain lambda
    • Whole Molecule
    • Chain mu
    • Chain alpha
    • Dimer
    • C-Term
    • Joining Chain
    • Secretory Component
    • Chain delta
    • Chain epsilon
    • N-Term
    • free
    • Chain gamma 1
    • Chain gamma 2a
    • Domain CH2
    • Fd Region
    • full length
    • AA 1-234
    • AA 1-469
    • AA 1-477
    • AA 150-178
    • Center
    • Chain gamma 2b
    • Fc5mu Region
    • Hinge Region
    • Internal Region
    • AA 1-159
    • AA 1-180
    • AA 1-232
    • AA 1-236
    • AA 1-330
    • AA 1-473
    • AA 1-489
    • AA 1-521
    • AA 11-98
    • AA 111-160
    • AA 112-206
    • AA 196-245
    • AA 222-327
    • AA 227-276
    • AA 23-159
    Please use search to find more...
  • 标记 un-conjugated
    • 非结合性
    • FITC
    • HRP
    • Biotin
    • TRITC
    • Alkaline Phosphatase (AP)
    • Texas Red (TR)
    • PE
    • DyLight 488
    • DyLight 550
    • DyLight 650
    • DyLight 800
    • DyLight 594
    • Cy5
    • Cy3
    • Cy5.5
    • DyLight 680
    • DyLight 405
    • CF®488A
    • Alexa Fluor 647
    • DyLight 549
    • Alexa Fluor 488
    • Alexa Fluor 594
    • DyLight 649
    • Alexa Fluor 568
    • Alexa Fluor 680
    • Alexa Fluor 750
    • APC
    • Alexa Fluor 350
    • Alexa Fluor 555
    • CF®594
    • Cy7
    • Alexa Fluor 405
    • Alexa Fluor 532
    • Alexa Fluor 633
    • Alexa Fluor 660
    • Alexa Fluor 790
    • CF®568
    • CF®640R
    • CF®543
    • CF®633
    • CF®647
    • CF®555
    • DyLight 633
    • CF®350
    • CF®680
    • DyLight 755
    • Agarose Beads
    • DyLight 350
    • CF®405S
    • SureLight® R-PE
  • 抗体亚型
    • IgG
    • IgG1
    • IgG2a
    • IgG2b
    • IgM
    • IgG1 kappa
    • IgY
    • IgG kappa
    • IgG2a kappa
    • IgG2b kappa
    • IgG3
    • Cocktail
    • Ig Fraction
    • IgA
  • 蛋白类型
    • Recombinant Antibody
  • Format
    • Liquid
    • Lyophilized
  • Fragment
    • F(ab')2 fragment
    • Fab fragment
    • Fc fragment
    • single-domain Antibody (sdAb)
  • Supplier
    • antibodies-online
    • Acris
    • USBio
    • RayBiotech
    • Abbexa
    • Cohesion Biosciences
    • Covalab
    • Assaypro
    • Cloud-Clone
    • NSJ Bioreagents
    • CUSABIO
    • ProSci
    • RevMAb Biosciences
    • QED Bioscience
    • neweramabs
    • ANTIGENES
    • Assay Biotechnology Company, Inc.
    • Biospes
    • Boster Bio
    • Enogene Biotech
    • Hytest
    • ImmunoBioscience
    • Synaptic Systems
    • Agrisera
    • Angio-Proteomie
    • AntiProt
    • Biotium
    • ITSI-Biosciences
    • NBS-C
    • PROGEN
    • Wuhan Fine biotech
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