SARS-CoV-2 Spike Protein (BA.4 - Omicron, BA.5 - Omicron, RBD) (His-Avi Tag,Biotin)
(1 reference)-
- 抗原 See all SARS-CoV-2 Spike 蛋白
- SARS-CoV-2 Spike
- 蛋白类型
- Recombinant
- 产品特性
- BA.4 - Omicron, BA.5 - Omicron, RBD
-
宿主
- SARS Coronavirus-2 (SARS-CoV-2), SARS CoV-2 Omicron
-
资源
- HEK-293 Cells
- 标记
- This SARS-CoV-2 Spike protein is labelled with His-Avi Tag,Biotin.
- 原理
- Biotinylated SARS-CoV-2 Spike RBD, His,Avitag™ (BA.4&BA.5/Omicron) (MALS verified)
- 序列
- Arg 319 - Lys 537
- 产品特性
- Biotinylated SARS-CoV-2 Spike RBD, His,Avitag (BA.4&BA.5/Omicron) is expressed from human 293 cells (HEK293). It contains AA Arg 319 - Lys 537 (Accession # QHD43416.1 (G339D, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, L452R, S477N, T478K, E484A, F486V, Q498R, N501Y, Y505H). The spike mutations are identified on the SARS-CoV-2 Omicron variant (Pango lineage: BA.4 and BA.5, GISAID clade: GRA).
- 纯度
- >95 % as determined by SDS-PAGE.
- 内毒素水平
- Less than 1.0 EU per μg by the LAL method.
- 质量等级
- MALS verified
- Top Product
- Discover our top product SARS-CoV-2 Spike 蛋白
-
SARS-CoV-2 Spike (Super Stable Trimer) protein (His tag)
宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE. > 90 % as determined by SEC-MALS. ELISA
SARS-CoV-2 Spike (Trimer) protein (rho-1D4 tag)宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB, Crys
SARS-CoV-2 Spike (B.1.351 - beta) protein (rho-1D4 tag)宿主: SARS Coronavirus-2 (SARS-CoV-2), SARS CoV-2 Beta 宿主: HEK-293 Cells Recombinant > 98 % as determined by SDS-PAGE ELISA, LBA
SARS-CoV-2 Spike (B.1.1.7 - alpha) protein (rho-1D4 tag)宿主: SARS Coronavirus-2 (SARS-CoV-2), SARS CoV-2 Alpha 宿主: HEK-293 Cells > 98 % as determined by SDS-PAGE ELISA, LBA
SARS-CoV-2 Spike (P.1 - gamma) protein (rho-1D4 tag)宿主: SARS Coronavirus-2 (SARS-CoV-2), SARS CoV-2 Gamma 宿主: HEK-293 Cells Recombinant > 98 % as determined by SDS-PAGE
SARS-CoV-2 Spike (RBD) protein (His-Avi Tag)宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: HEK-293 Cells Recombinant > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
SARS-CoV-2 Spike (N501Y), (RBD) protein (His tag)宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
SARS-CoV-2 Spike (E484K), (K417N), (N501Y), (RBD) protein (His tag)宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
SARS-CoV-2 Spike (RBD) protein (mFc-His Tag)宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 90 % as determined by SDS-PAGE and Coomassie blue staining. ELISA, WB
SARS-CoV-2 Spike (B.1.617.1 - kappa) protein (rho-1D4 tag)宿主: SARS Coronavirus-2 (SARS-CoV-2), SARS CoV-2 Kappa 宿主: HEK-293 Cells Recombinant > 98% as determined by SDS-PAGE ELISA, LBA
-
- 应用备注
- This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™). The protein has a calculated MW of 28.3 kDa. The protein migrates as 34-37 kDa under reducing (R) condition due to glycosylation.
- 说明
-
Ready-to-use Avitag™ biotinylated protein:
The product is exclusively produced using the Avitag™ technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.
This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled. - 限制
- 仅限研究用
-
- 状态
- Lyophilized
- 缓冲液
- PBS, pH 7.4
- 储存条件
- -20 °C
- 储存方法
- For long term storage, the product should be stored at lyophilized state at -20°C or lower.
-
-
: "A Spike-destructing human antibody effectively neutralizes Omicron-included SARS-CoV-2 variants with therapeutic efficacy." in: PLoS pathogens, Vol. 19, Issue 1, pp. e1011085, (2023) (PubMed).
-
: "A Spike-destructing human antibody effectively neutralizes Omicron-included SARS-CoV-2 variants with therapeutic efficacy." in: PLoS pathogens, Vol. 19, Issue 1, pp. e1011085, (2023) (PubMed).
-
- 抗原
- SARS-CoV-2 Spike
- 别名
- SARS-CoV-2 Spike (SARS-CoV-2 Spike 产品)
- 别名
- E2 Protein, E2 glycoprotein precursor Protein, Surface Glycoprotein Protein, S Protein
- 背景
-
Synonyms: Spike,S protein RBD,Spike glycoprotein Receptor-binding domain,S glycoprotein RBD,Spike protein RBD,
Description: It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. - 分子量
- 28.3 kDa
-
