SARS-CoV-2 Spike Protein (B.1.1.7 - alpha) (rho-1D4 tag)
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- Key Features
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- Recombinant trimeric S protein of the SARS-CoV-2 variant of concern B.1.1.7.
- Contains del 69-70, del 144, N501Y, A570D, D614G, P681H, T716I, S982A, and D1118H mutations characteristic for the SARS-CoV-2 alpha variant (B.1.1.7).
- 抗原 See all SARS-CoV-2 Spike 蛋白
- SARS-CoV-2 Spike
- 产品特性
- B.1.1.7 - alpha
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宿主
- SARS Coronavirus-2 (SARS-CoV-2), SARS CoV-2 Alpha
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资源
- HEK-293 Cells
- 标记
- This SARS-CoV-2 Spike protein is labelled with rho-1D4 tag.
- 应用范围
- ELISA, Ligand Binding Assay (LBA)
- 原理
- This is the spike protein of the mutant strain B.1.1.7, also commonly known as the "UK / Great Britain mutant".
- 序列
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Full-length sequence (aa 1 - 1273), del 69, del 70, del 144, N501Y, A570D, D614G, P681H, T716I, S982A, D1118H
furin cleavage site ""RRAR"" mutated to ""GSAG"", KV986PP
C-terminal Rho1D4 tag fused with spacer ""GSSG"" to protein sequence - 特异性
- Mutation that differ from canonical sequence of SPIKE protein: del 69-70, del 144, N501Y, A570D, D614G, P681H, T716I, S982A, D1118H
- 产品特性
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"SARS CoV-2 full-length Spike B.1.1.7 Mutation"
All viruses undergo fast mutations and adept quickly to the countermeasures that the immune systems creates against them. SARS-CoV-2 of the COVID-19 pandemic is no exception here. During the pandemic multiple mutant strains arose. To help the science combat these mutants we offer the SPIKE protein of these mutants in full-length and active in its native trimeric form, stabilized with the LMNG detergent. - 纯化方法
- affinity chromatography
- 纯度
- > 98 % as determined by SDS-PAGE
- Top Product
- Discover our top product SARS-CoV-2 Spike 蛋白
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SARS-CoV-2 Spike (Super Stable Trimer) protein (His tag)
宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE. > 90 % as determined by SEC-MALS. ELISA
SARS-CoV-2 Spike (Trimer) protein (rho-1D4 tag)宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB, Crys
SARS-CoV-2 Spike (B.1.351 - beta) protein (rho-1D4 tag)宿主: SARS Coronavirus-2 (SARS-CoV-2), SARS CoV-2 Beta 宿主: HEK-293 Cells Recombinant > 98 % as determined by SDS-PAGE ELISA, LBA
SARS-CoV-2 Spike (P.1 - gamma) protein (rho-1D4 tag)宿主: SARS Coronavirus-2 (SARS-CoV-2), SARS CoV-2 Gamma 宿主: HEK-293 Cells Recombinant > 98 % as determined by SDS-PAGE
SARS-CoV-2 Spike (RBD) protein (His-Avi Tag)宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: HEK-293 Cells Recombinant > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
SARS-CoV-2 Spike (N501Y), (RBD) protein (His tag)宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
SARS-CoV-2 Spike (E484K), (K417N), (N501Y), (RBD) protein (His tag)宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
SARS-CoV-2 Spike (RBD) protein (mFc-His Tag)宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 90 % as determined by SDS-PAGE and Coomassie blue staining. ELISA, WB
SARS-CoV-2 Spike (B.1.617.1 - kappa) protein (rho-1D4 tag)宿主: SARS Coronavirus-2 (SARS-CoV-2), SARS CoV-2 Kappa 宿主: HEK-293 Cells Recombinant > 98% as determined by SDS-PAGE ELISA, LBA
SARS-CoV-2 Spike (Super Stable Trimer) protein (His tag)宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE. > 90 % as determined by SEC-MALS. ELISA
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- 应用备注
- ELISA assays, Ligand binding assays (e.g. SPR), Biochemical and biophysical analyses
- 说明
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Further modifications:
- furin cleavage site "682-RRAR|SV-687" mutated to "682-GSAG|PP-687"
- C-terminal Rho1D4 tag fused with spacer "GSSG" to protein sequence
Size: 1286 amino acids (including Rho1D4 tag and linker) - 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 20 mM Hepes pH 7.5, 150 mM NaCl, 0.001 % LMNG
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C. Avoid freeze-thaw cycles
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: "Nanoplasmonic biosensor for rapid detection of multiple viral variants in human serum." in: Sensors and actuators. B, Chemical, Vol. 365, pp. 131906, (2022) (PubMed).
: "Measuring the Radius of Gyration and Intrinsic Flexibility of Viral Proteins in Buffer Solution Using Small-Angle X-ray Scattering." in: ACS measurement science au, Vol. 2, Issue 6, pp. 547-552, (2022) (PubMed).
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: "Nanoplasmonic biosensor for rapid detection of multiple viral variants in human serum." in: Sensors and actuators. B, Chemical, Vol. 365, pp. 131906, (2022) (PubMed).
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- 抗原
- SARS-CoV-2 Spike
- 别名
- SARS2 Spike glycoprotein (SARS-CoV-2 Spike 产品)
- 别名
- E2 Protein, E2 glycoprotein precursor Protein, Surface Glycoprotein Protein, S Protein
- 物质类
- Viral Protein
- 分子量
- 142114 Da
- UniProt
- P0DTC2
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