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SARS-CoV-2 Spike Protein (B.1.351 - beta) (rho-1D4 tag)

Name: SARS-CoV-2 Spike Protein (B.1.351 - beta) (rho-1D4 tag)
Brand: antibodies-online
SKU: ABIN6963740
Availability: OutOfStock

宿主: SARS Coronavirus-2 (SARS-CoV-2), SARS CoV-2 Beta 宿主: HEK-293 Cells Recombinant > 98 % as determined by SDS-PAGE ELISA, LBA

SDS-Page of purified SPIKE in detergent mycelle.

SDS

Bind

Western blot of Rho1d4 tagged SPIKE, originated from the SDS Page.

4 images

(4 references)

产品编号 ABIN6963740

发货至: 中国

Datasheet as PDF

抗原 See all SARS-CoV-2 Spike 蛋白

SARS-CoV-2 Spike

蛋白类型

Recombinant

产品特性

B.1.351 - beta
宿主
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SARS Coronavirus-2 (SARS-CoV-2), SARS CoV-2 Beta
资源
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HEK-293 Cells

标记

This SARS-CoV-2 Spike protein is labelled with rho-1D4 tag.

应用范围

ELISA, Ligand Binding Assay (LBA)

原理

This is the spike protein of the mutant strain B.1.351, also commonly known as the "South Africa mutant".

特异性

Mutation that differ from canonical sequence of SPIKE protein: del 144, K417N, E484K, N501Y, A570D, D614G, P681H, T716I, S982A, D1118H

产品特性

"SARS CoV-2 full-length Spike B.1.351 Mutation"
All viruses undergo fast mutations and adept quickly to the countermeasures that the immune systems creates against them. SARS-CoV-2 of the COVID-19 pandemic is no exception here. During the pandemic multiple mutant strains arose. To help the science combat these mutants we offer the SPIKE protein of these mutants in full-length and active in its native trimeric form, stabilized with the LMNG detergent.

纯化方法

affinity chromatography

纯度

> 98 % as determined by SDS-PAGE

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宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE. > 90 % as determined by SEC-MALS. ELISA

SARS-CoV-2 Spike (Trimer) protein (rho-1D4 tag)
宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB, Crys

SARS-CoV-2 Spike (B.1.1.7 - alpha) protein (rho-1D4 tag)
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SARS-CoV-2 Spike (P.1 - gamma) protein (rho-1D4 tag)
宿主: SARS Coronavirus-2 (SARS-CoV-2), SARS CoV-2 Gamma 宿主: HEK-293 Cells Recombinant > 98 % as determined by SDS-PAGE

SARS-CoV-2 Spike (RBD) protein (His-Avi Tag)
宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: HEK-293 Cells Recombinant > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC

SARS-CoV-2 Spike (N501Y), (RBD) protein (His tag)
宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

SARS-CoV-2 Spike (E484K), (K417N), (N501Y), (RBD) protein (His tag)
宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

SARS-CoV-2 Spike (RBD) protein (mFc-His Tag)
宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 90 % as determined by SDS-PAGE and Coomassie blue staining. ELISA, WB

SARS-CoV-2 Spike (B.1.617.1 - kappa) protein (rho-1D4 tag)
宿主: SARS Coronavirus-2 (SARS-CoV-2), SARS CoV-2 Kappa 宿主: HEK-293 Cells Recombinant > 98% as determined by SDS-PAGE ELISA, LBA

SARS-CoV-2 Spike (Super Stable Trimer) protein (His tag)
宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE. > 90 % as determined by SEC-MALS. ELISA

应用备注

ELISA assays, Ligand binding assays (e.g. SPR), Biochemical and biophysical analyses

说明

Further modifications:
- furin cleavage site "682-RRAR|SV-687" mutated to "682-GSAG|PP-687"
- C-terminal Rho1D4 tag fused with spacer "GSSG" to protein sequence
Size: 1286 amino acids (including Rho1D4 tag and linker)

限制

仅限研究用
状态

Liquid

缓冲液

20 mM Hepes pH 7.5, 150 mM NaCl, 0.001 % LMNG

储存条件

-80 °C

储存方法

Store at -80°C. Avoid freeze-thaw cycles
Erickson, Logsdon, Rhea, Hansen, Holden, Banks, Smith, German, Farr, Morley, Weaver, Hirsch, Kovac, Kontsekova, Baumann, Omer, Raber: "Blood-brain barrier penetration of non-replicating SARS-CoV-2 and S1 variants of concern induce neuroinflammation which is accentuated in a mouse model of Alzheimer's disease." in: Brain, behavior, and immunity, Vol. 109, pp. 251-268, (2023) (PubMed).

Bhalla, Payam, Morelli, Sharma, Johnson, Thomson, Jolly, Canfarotta: "Nanoplasmonic biosensor for rapid detection of multiple viral variants in human serum." in: Sensors and actuators. B, Chemical, Vol. 365, pp. 131906, (2022) (PubMed).

Arunachalam, Feng, Ashraf, Hu, Walls, Edara, Zarnitsyna, Aye, Golden, Miranda, Green, Threeton, Maness, Beddingfield, Bohm, Scheuermann, Goff, Dufour, Russell-Lodrigue, Kepl, Fiala, Wrenn et al.: "Durable protection against the SARS-CoV-2 Omicron variant is induced by an adjuvanted subunit vaccine. ..." in: Science translational medicine, Vol. 14, Issue 658, pp. eabq4130, (2022) (PubMed).

Funari, Bhalla, Gentile: "Measuring the Radius of Gyration and Intrinsic Flexibility of Viral Proteins in Buffer Solution Using Small-Angle X-ray Scattering." in: ACS measurement science au, Vol. 2, Issue 6, pp. 547-552, (2022) (PubMed).
抗原

SARS-CoV-2 Spike

别名

SARS2 Spike glycoprotein (SARS-CoV-2 Spike 产品)

别名

E2 Protein, E2 glycoprotein precursor Protein, Surface Glycoprotein Protein, S Protein

物质类

Viral Protein

分子量

142114 Da

UniProt

P0DTC2