SARS-CoV-2 Spike Protein (E484K, K417N, N501Y, RBD) (His tag)
-
- 抗原 See all SARS-CoV-2 Spike 蛋白
- SARS-CoV-2 Spike
- 蛋白类型
- Recombinant
- 产品特性
- E484K, K417N, N501Y, RBD
-
宿主
- SARS Coronavirus-2 (SARS-CoV-2)
-
资源
- HEK-293 Cells
- 标记
- This SARS-CoV-2 Spike protein is labelled with His tag.
- 原理
- SARS-COV-2 Spike RBD (N501Y,K417N,E484K) Protein
- 序列
- Arg319-Phe541 (N501Y,K417N,E484K)
- 产品特性
- Recombinant SARS-COV-2 Spike RBD (N501Y,K417N,E484K) Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Arg319-Phe541(N501Y,K417N,E484K).
- 纯度
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized SARS-COV-2 Spike RBD (N501Y,K417N,E484K) , His Tag at 0.5μg/ml (100ul/Well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 23.3ng/ml determined by ELISA. See testing image for detail.
- Top Product
- Discover our top product SARS-CoV-2 Spike 蛋白
-
-
- 限制
- 仅限研究用
-
- 状态
- Lyophilized
- 溶解方式
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- 缓冲液
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- 储存条件
- -20 °C,-80 °C
- 储存方法
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- 有效期
- 12 months
-
- 抗原
- SARS-CoV-2 Spike
- Abstract
- SARS-CoV-2 Spike 产品
- 别名
- E2 Protein, E2 glycoprotein precursor Protein, Surface Glycoprotein Protein, S Protein
- 物质类
- Viral Protein
- 背景
- The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
- 分子量
- 26.2 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Tris-Bis PAGE result.
-