SARS-CoV-2 Spike Protein (Super Stable Trimer) (His tag)
(2 references)-
- 抗原 See all SARS-CoV-2 Spike 蛋白
- SARS-CoV-2 Spike
- 蛋白类型
- Recombinant
- 产品特性
- Super Stable Trimer
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宿主
- SARS Coronavirus-2 (SARS-CoV-2)
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资源
- HEK-293 Cells
- 标记
- This SARS-CoV-2 Spike protein is labelled with His tag.
- 应用范围
- ELISA
- 原理
- SARS-CoV-2 S protein, His Tag, Super stable trimer (MALS & NS-EM verified)
- 序列
- AA 16-1213
- 产品特性
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SARS-CoV-2 S protein, His Tag, Super stable trimer is the ectodomain of SARS-CoV-2 S protein which contains AA Val 16 - Pro 1213 (Accession # QHD43416.1). The recombinant protein is expressed from human 293 cells (HEK293) with T4 fibritin trimerization motif and a polyhistidine tag at the C-terminus.
Proline substitutions (F817P, A892P, A899P, A942P, K986P, V987P) and alanine substitutions (R683A and R685A) are introduced to stabilize the trimeric prefusion state of SARS-CoV-2 S protein and abolish the furin cleavage site, respectively. - 纯度
- > 95 % as determined by SDS-PAGE. > 90 % as determined by SEC-MALS.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Less than 1.0 EU per μg by the LAL method.
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SARS-CoV-2 Spike (Trimer) protein (rho-1D4 tag)
宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB, Crys
SARS-CoV-2 Spike (B.1.351 - beta) protein (rho-1D4 tag)宿主: SARS Coronavirus-2 (SARS-CoV-2), SARS CoV-2 Beta 宿主: HEK-293 Cells Recombinant > 98 % as determined by SDS-PAGE ELISA, LBA
SARS-CoV-2 Spike (B.1.1.7 - alpha) protein (rho-1D4 tag)宿主: SARS Coronavirus-2 (SARS-CoV-2), SARS CoV-2 Alpha 宿主: HEK-293 Cells > 98 % as determined by SDS-PAGE ELISA, LBA
SARS-CoV-2 Spike (P.1 - gamma) protein (rho-1D4 tag)宿主: SARS Coronavirus-2 (SARS-CoV-2), SARS CoV-2 Gamma 宿主: HEK-293 Cells Recombinant > 98 % as determined by SDS-PAGE
SARS-CoV-2 Spike (RBD) protein (His-Avi Tag)宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: HEK-293 Cells Recombinant > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
SARS-CoV-2 Spike (N501Y), (RBD) protein (His tag)宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
SARS-CoV-2 Spike (E484K), (K417N), (N501Y), (RBD) protein (His tag)宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
SARS-CoV-2 Spike (RBD) protein (mFc-His Tag)宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 90 % as determined by SDS-PAGE and Coomassie blue staining. ELISA, WB
SARS-CoV-2 Spike (B.1.617.1 - kappa) protein (rho-1D4 tag)宿主: SARS Coronavirus-2 (SARS-CoV-2), SARS CoV-2 Kappa 宿主: HEK-293 Cells Recombinant > 98% as determined by SDS-PAGE ELISA, LBA
SARS-CoV-2 Spike (Super Stable Trimer) protein (His tag)宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE. > 90 % as determined by SEC-MALS. ELISA
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- 应用备注
- This product is specifically designed for antibody drug development.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 缓冲液
- PBS
- 储存条件
- -20 °C,-80 °C
- 储存方法
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For long term storage, the product should be stored at lyophilized state at -20°C or lower.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state
70°C for 3 months under sterile conditions after reconstitution. - 有效期
- 12 months
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: "Nano-size dependence in the adsorption by the SARS-CoV-2 spike protein over gold colloid." in: Colloids and surfaces. A, Physicochemical and engineering aspects, Vol. 615, pp. 126275, (2021) (PubMed).
: "HDL-scavenger receptor B type 1 facilitates SARS-CoV-2 entry." in: Nature metabolism, Vol. 2, Issue 12, pp. 1391-1400, (2020) (PubMed).
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: "Nano-size dependence in the adsorption by the SARS-CoV-2 spike protein over gold colloid." in: Colloids and surfaces. A, Physicochemical and engineering aspects, Vol. 615, pp. 126275, (2021) (PubMed).
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- 抗原
- SARS-CoV-2 Spike
- Abstract
- SARS-CoV-2 Spike 产品
- 别名
- E2 Protein, E2 glycoprotein precursor Protein, Surface Glycoprotein Protein, S Protein
- 物质类
- Viral Protein
- 背景
- It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
- 分子量
- 138.0 kDa
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