SARS-CoV-2 Spike Protein (B.1.351 - beta, Trimer) (His tag)
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- 抗原 See all SARS-CoV-2 Spike 蛋白
- SARS-CoV-2 Spike
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- B.1.351 - beta, Trimer
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宿主
- SARS Coronavirus-2 (SARS-CoV-2), SARS CoV-2 Beta
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资源
- HEK-293 Cells
- 标记
- This SARS-CoV-2 Spike protein is labelled with His tag.
- 原理
- SARS-CoV-2 S protein (L18F, D80A, D215G, 242-244del, R246I, K417N, E484K, N501Y, D614G, A701V) trimer, His Tag (MALS verified)
- 序列
- AA 16-1213
- 产品特性
- SARS-CoV-2 S protein trimer, His Tag is expressed from human 293 cells (HEK293). It contains AA Val 16 - Pro 1213 (Accession # QHD43416.1). The recombinant protein is expressed from human 293 cells (HEK293) with T4 fibritin trimerization motif and a polyhistidine tag at the C-terminus. Proline substitutions (F817P/ A892P/ A899P/ A942P/ K986P/ V987P) and alanine substitutions (R683A and R685A) are introduced to stabilize the trimeric prefusion state of SARS-CoV-2 S protein and abolish the furin cleavage site, respectively. L18F/ D80A/ D215G/ LAL242-244del/ R246I/ K417N/ E484K/ N501Y/ D614G/ A701Vmutations were identified on the spike protein in the SARS-CoV-2 variant (known as B.1.351 or 20C/501Y.V2) which emerged in South Africa.
- 纯度
- >90 % as determined by SDS-PAGE.
- 内毒素水平
- Less than 1.0 EU per μg by the LAL method.
- Top Product
- Discover our top product SARS-CoV-2 Spike 蛋白
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SARS-CoV-2 Spike (Super Stable Trimer) protein (His tag)
宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE. > 90 % as determined by SEC-MALS. ELISA
SARS-CoV-2 Spike (Trimer) protein (rho-1D4 tag)宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS, Crys
SARS-CoV-2 Spike (B.1.351 - beta) protein (rho-1D4 tag)宿主: SARS Coronavirus-2 (SARS-CoV-2), SARS CoV-2 Beta 宿主: HEK-293 Cells Recombinant > 98 % as determined by SDS-PAGE ELISA, LBA
SARS-CoV-2 Spike (B.1.1.7 - alpha) protein (rho-1D4 tag)宿主: SARS Coronavirus-2 (SARS-CoV-2), SARS CoV-2 Alpha 宿主: HEK-293 Cells > 98 % as determined by SDS-PAGE ELISA, LBA
SARS-CoV-2 Spike (P.1 - gamma) protein (rho-1D4 tag)宿主: SARS Coronavirus-2 (SARS-CoV-2), SARS CoV-2 Gamma 宿主: HEK-293 Cells Recombinant > 98 % as determined by SDS-PAGE
SARS-CoV-2 Spike (RBD) protein (His-Avi Tag)宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: HEK-293 Cells Recombinant > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
SARS-CoV-2 Spike (N501Y), (RBD) protein (His tag)宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
SARS-CoV-2 Spike (E484K), (K417N), (N501Y), (RBD) protein (His tag)宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
SARS-CoV-2 Spike (RBD) protein (mFc-His Tag)宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 90 % as determined by SDS-PAGE and Coomassie blue staining. ELISA, WB
SARS-CoV-2 Spike (B.1.617.1 - kappa) protein (rho-1D4 tag)宿主: SARS Coronavirus-2 (SARS-CoV-2), SARS CoV-2 Kappa 宿主: HEK-293 Cells Recombinant > 98% as determined by SDS-PAGE ELISA, LBA
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 缓冲液
- PBS
- 储存条件
- -20 °C
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- 抗原
- SARS-CoV-2 Spike
- 别名
- SARS-CoV-2 S protein (SARS-CoV-2 Spike 产品)
- 别名
- E2 Protein, E2 glycoprotein precursor Protein, Surface Glycoprotein Protein, S Protein
- 物质类
- Viral Protein
- 背景
- It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
- 分子量
- 137.6 kDa
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