SARS-CoV-2 Spike Protein (ECD) (His tag)
(1 reference)-
- 抗原 See all SARS-CoV-2 Spike 蛋白
- SARS-CoV-2 Spike
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- ECD
-
宿主
- SARS Coronavirus-2 (SARS-CoV-2)
-
资源
- HEK-293 Cells
- 标记
- This SARS-CoV-2 Spike protein is labelled with His tag.
- 应用范围
- SDS-PAGE (SDS), Functional Studies (Func)
- 序列
- VSSQCVNLTT RTQLPPAYTN SFTRGVYYPD KVFRSSVLHS TQDLFLPFFS NVTWFHAIHV SGTNGTKRFD NPVLPFNDGV YFASTEKSNI IRGWIFGTTL DSKTQSLLIV NNATNVVIKV CEFQFCNDPF LGVYYHKNNK SWMESEFRVY SSANNCTFEY VSQPFLMDLE GKQGNFKNLR EFVFKNIDGY FKIYSKHTPI NLVRDLPQGF SALEPLVDLP IGINITRFQT LLALHRSYLT PGDSSSGWTA GAAAYYVGYL QPRTFLLKYN ENGTITDAVD CALDPLSETK CTLKSFTVEK GIYQTSNFRV QPTESIVRFP NITNLCPFGE VFNATRFASV YAWNRKRISN CVADYSVLYN SASFSTFKCY GVSPTKLNDL CFTNVYADSF VIRGDEVRQI APGQTGKIAD YNYKLPDDFT GCVIAWNSNN LDSKVGGNYN YLYRLFRKSN LKPFERDIST EIYQAGSTPC NGVEGFNCYF PLQSYGFQPT NGVGYQPYRV VVLSFELLHA PATVCGPKKS TNLVKNKCVN FNFNGLTGTG VLTESNKKFL PFQQFGRDIA DTTDAVRDPQ TLEILDITPC SFGGVSVITP GTNTSNQVAV LYQDVNCTEV PVAIHADQLT PTWRVYSTGS NVFQTRAGCL IGAEHVNNSY ECDIPIGAGI CASYQTQTNS PRRARSVASQ SIIAYTMSLG AENSVAYSNN SIAIPTNFTI SVTTEILPVS MTKTSVDCTM YICGDSTECS NLLLQYGSFC TQLNRALTGI AVEQDKNTQE VFAQVKQIYK TPPIKDFGGF NFSQILPDPS KPSKRSFIED LLFNKVTLAD AGFIKQYGDC LGDIAARDLI CAQKFNGLTV LPPLLTDEMI AQYTSALLAG TITSGWTFGA GAALQIPFAM QMAYRFNGIG VTQNVLYENQ KLIANQFNSA IGKIQDSLSS TASALGKLQD VVNQNAQALN TLVKQLSSNF GAISSVLNDI LSRLDKVEAE VQIDRLITGR LQSLQTYVTQ QLIRAAEIRA SANLAATKMS ECVLGQSKRV DFCGKGYHLM SFPQSAPHGV VFLHVTYVPA QEKNFTTAPA ICHDGKAHFP REGVFVSNGT HWFVTQRNFY EPQIITTDNT FVSGNCDVVI GIVNNTVYDP LQPELDSFKE ELDKYFKNHT SPDVDLGDIS GINASVVNIQ KEIDRLNEVA KNLNESLIDL QELGKYEQ
- 特异性
- Val11-Gln1208
- 产品特性
- Recombinant 2019-nCoV S1+S2 ECD (S-ECD) Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Val11-Gln1208) of 2019-ncov S1+S2 ECD (S-ECD) (Accession #YP_009724390.1) fused with a 6xHis tag at the C-terminus.
- 纯度
- >97 % by SDS-PAGE.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- < 0.1 EU/μg of the protein by LAL method.
- Biological Activity Comment
- Measured by its binding ability in a functional ELISA. Immobilized Recombinant SARS-CoV-2 Spike S1+S2 ECD-His at 2 μg/mL (100 μL/well) can bind Recombinant Human ACE2 with a linear range of 0.15-9.92 ng/mL.
- Top Product
- Discover our top product SARS-CoV-2 Spike 蛋白
-
SARS-CoV-2 Spike (Super Stable Trimer) protein (His tag)
宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE. > 90 % as determined by SEC-MALS. ELISA
SARS-CoV-2 Spike (Trimer) protein (rho-1D4 tag)宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB, Crys
SARS-CoV-2 Spike (B.1.351 - beta) protein (rho-1D4 tag)宿主: SARS Coronavirus-2 (SARS-CoV-2), SARS CoV-2 Beta 宿主: HEK-293 Cells Recombinant > 98 % as determined by SDS-PAGE ELISA, LBA
SARS-CoV-2 Spike (B.1.1.7 - alpha) protein (rho-1D4 tag)宿主: SARS Coronavirus-2 (SARS-CoV-2), SARS CoV-2 Alpha 宿主: HEK-293 Cells > 98 % as determined by SDS-PAGE ELISA, LBA
SARS-CoV-2 Spike (P.1 - gamma) protein (rho-1D4 tag)宿主: SARS Coronavirus-2 (SARS-CoV-2), SARS CoV-2 Gamma 宿主: HEK-293 Cells Recombinant > 98 % as determined by SDS-PAGE
SARS-CoV-2 Spike (RBD) protein (His-Avi Tag)宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: HEK-293 Cells Recombinant > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
SARS-CoV-2 Spike (N501Y), (RBD) protein (His tag)宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
SARS-CoV-2 Spike (E484K), (K417N), (N501Y), (RBD) protein (His tag)宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
SARS-CoV-2 Spike (RBD) protein (mFc-His Tag)宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 90 % as determined by SDS-PAGE and Coomassie blue staining. ELISA, WB
SARS-CoV-2 Spike (B.1.617.1 - kappa) protein (rho-1D4 tag)宿主: SARS Coronavirus-2 (SARS-CoV-2), SARS CoV-2 Kappa 宿主: HEK-293 Cells Recombinant > 98% as determined by SDS-PAGE ELISA, LBA
-
- 限制
- 仅限研究用
-
- 状态
- Liquid
- 浓度
- 0.95 mg/mL
- 缓冲液
- Supplied as a 0.22 μm filtered solution in PBS, pH 7.4.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- This product is stable at ≤ -70°C for up to 6 months from the date of receipt. |For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated freeze-thaw cycles.
-
-
: "SARS-CoV-2 neutralizing camelid heavy-chain-only antibodies as powerful tools for diagnostic and therapeutic applications." in: Frontiers in immunology, Vol. 13, pp. 930975, (2022) (PubMed).
-
: "SARS-CoV-2 neutralizing camelid heavy-chain-only antibodies as powerful tools for diagnostic and therapeutic applications." in: Frontiers in immunology, Vol. 13, pp. 930975, (2022) (PubMed).
-
- 抗原
- SARS-CoV-2 Spike
- Abstract
- SARS-CoV-2 Spike 产品
- 别名
- E2 Protein, E2 glycoprotein precursor Protein, Surface Glycoprotein Protein, S Protein
- 物质类
- Viral Protein
- 背景
-
Description: The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates thisinteraction.The S protein plays key parts in the induction of neutralizing-antibody and T-cellresponses, as well as protective immunity.
Name: Envelope,SARS-CoV-2 Spike RBD (N501Y),Spike,Spike ECD,Spike RBD,Spike S1,Spike S2,Spike S2 ECD,S1-RBD protein,NCP-CoV RBD Protein,novel coronavirus RBD Protein,2019-nCoV RBD Protein,S glycoprotein Subunit1 RBD Protein
- 基因ID
- 43740568
- UniProt
- P0DTC2
-
