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Independently validated

Here you can find our antibodies which have been validated through our Independent Validation Initiative(IVI).

The IVI is centered around core values of transparency, reproducibility, scientific integrity. We are committed to improving the antibody and antibody market by providing independently collected, scientifically sound, and properly documented data for the products that we sell, which is why we set very high standards for independent validation data. Our support can answer further questions via phone, live chat or email.

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235 results :

NFKB1 抗体 (AA 740-964)

NFKB1 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

Immunofluorescence validation image for anti-Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells 1 (NFKB1) (AA 740-964) antibody (ABIN6144571)

Western blot analysis of extracts of various cell lines, using NFKB1 antibody.

Immunofluorescence analysis of U2OS cells using NFKB1 Rabbit pAb (ABIN6130581, ABIN6144571, ABIN6144573 and ABIN7101802) at dilution of 1:50 (40x lens).

Immunohistochemistry of paraffin-embedded mouse lung using NFKB1 antibody.

Immunofluorescence analysis of A549 cells using NFKB1 antibody.

Immunofluorescence analysis of A-549 cells using NFKB1 Rabbit pAb (ABIN6130581, ABIN6144571, ABIN6144573 and ABIN7101802) at dilution of 1:50 (40x lens).

Immunohistochemistry of paraffin-embedded rat lung using NFKB1 Rabbit pAb (ABIN6130581, ABIN6144571, ABIN6144573 and ABIN7101802) at dilution of 1:50 (40x lens).

Immunofluorescence analysis of NIH/3T3 cells using NFKB1 Rabbit pAb (ABIN6130581, ABIN6144571, ABIN6144573 and ABIN7101802) at dilution of 1:50 (40x lens).

8 images

2 references

Independent Validation

1 validation

产品编号 ABIN6144571

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Datasheet as PDF

Complement Factor H 抗体 (AA 20-270)

CFH 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

Immunofluorescence validation image for anti-Complement Factor H (CFH) (AA 20-270) antibody (ABIN3023097)

Western blot analysis of extracts of HepG2 cell line, using CFH antibody.

Immunohistochemistry of paraffin-embedded human placenta using Complement factor H Rabbit pAb (ABIN3023096, ABIN3023097, ABIN3023098 and ABIN6219387) at dilution of 1:100 (40x lens).

Western blot analysis of extracts of Rat liver, using Complement factor H antibody (ABIN3023096, ABIN3023097, ABIN3023098 and ABIN6219387) at 1:1000 dilution.

Western blot analysis of extracts of Human plasma, using Complement factor H antibody (ABIN3023096, ABIN3023097, ABIN3023098 and ABIN6219387) at 1:1000 dilution.

Western blot analysis of extracts of various cell lines, using Complement factor H antibody (ABIN3023096, ABIN3023097, ABIN3023098 and ABIN6219387) at 1:1000 dilution.

Immunohistochemistry of paraffin-embedded human liver cancer using Complement factor H Rabbit pAb (ABIN3023096, ABIN3023097, ABIN3023098 and ABIN6219387) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of HepG2 cells using Complement factor H Rabbit pAb (ABIN3023096, ABIN3023097, ABIN3023098 and ABIN6219387) at dilution of 1:100 (40x lens).

8 images

1 reference

Independent Validation

1 validation

产品编号 ABIN3023097

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Datasheet as PDF

Histone 3 抗体 (H3K4me)

H3 适用: 人 WB, IF, ChIP, IP, ChIP-seq, CUT&RUN 宿主: 兔 Polyclonal unconjugated

Cleavage Under Targets and Release Using Nuclease validation image for anti-Histone 3 (H3) (H3K4me) antibody (ABIN3023251)

Cleavage Under Targets and Release Using Nuclease validation image for anti-Histone 3 (H3) (H3K4me) antibody (ABIN3023251)

Western blot analysis of extracts of various cell lines, using DiMethyl-Histone H3-K4 antibody.

Immunohistochemistry of paraffin-embedded human kidney cancer tissue using H3K4me2 antibody at dilution of 1:200 (x400 lens).

Immunohistochemistry of paraffin-embedded Mouse lung using DiMethyl-Histone H3-K4 antibody.

Immunohistochemistry of paraffin-embedded Rat brain using H3K4me2 antibody at dilution of 1:100 (x400 lens).

Immunohistochemistry of paraffin-embedded Rat kidney using H3K4me2 antibody at dilution of 1:100 (x400 lens).

Immunohistochemistry of paraffin-embedded Rat brain using DiMethyl-Histone H3-K4 antibody.

Dot Blot (DB) image for anti-Histone 3 (H3) (H3K4me) antibody (ABIN3023251)

Immunohistochemistry of paraffin-embedded Rat kidney using DiMethyl-Histone H3-K4 antibody.

Immunohistochemistry of paraffin-embedded Mouse brain using H3K4me2 antibody at dilution of 1:100 (x400 lens).

Dot-blot analysis of all sorts of methylation peptides using DiMethyl-Histone H3-K4 antibody.

Immunohistochemistry of paraffin-embedded Rat liver using DiMethyl-Histone H3-K4 antibody.

Immunohistochemistry of paraffin-embedded Rat liver using H3K4me2 antibody at dilution of 1:100 (x400 lens).

Immunohistochemistry of paraffin-embedded rat lung using DiMethyl-Histone H3-K4 antibody.

Immunofluorescence analysis of 293T cells using DiMethyl-Histone H3-K4 antibody.

Immunohistochemistry (IHC) image for anti-Histone 3 (H3) (H3K4me) antibody (ABIN3023251)

Immunohistochemistry of paraffin-embedded human lung cancer using DiMethyl-Histone H3-K4 antibody.

Chromatin immunoprecipitation analysis extracts of 293 cell line, using DiMethyl-Histone H3-K4 antibody.

Immunohistochemistry (IHC) image for anti-Histone 3 (H3) (H3K4me) antibody (ABIN3023251)

Immunohistochemistry of paraffin-embedded human kidney cancer using DiMethyl-Histone H3-K4 antibody.

Immunohistochemistry of paraffin-embedded Mouse lung using H3K4me2 antibody at dilution of 1:100 (x400 lens).

Immunohistochemistry of paraffin-embedded Mouse brain using DiMethyl-Histone H3-K4 antibody.

23 images

4 references

Independent Validation

1 validation

产品编号 ABIN3023251

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Datasheet as PDF

CBP 抗体 (C-Term)

KO Validated CREBBP 适用: 人 WB, IF, IP, IHC (p), ICC, ChIP 宿主: 兔 Polyclonal unconjugated

Cleavage Under Targets and Release Using Nuclease validation image for anti-CREB Binding Protein (CREBBP) (C-Term) antibody (ABIN2854987)

Cleavage Under Targets and Release Using Nuclease validation image for anti-CREB Binding Protein (CREBBP) (C-Term) antibody (ABIN2854987)

WB Image Sample (30 ug of whole cell lysate) A: H1299 5% SDS PAGE antibody diluted at 1:1000

IHC-P Image CBP antibody [C3], C-term detects CBP protein at nucleus on mouse skin by immunohistochemical analysis.

IHC-P Image CBP antibody [C3], C-term detects CBP protein at nucleus on human breast carcinoma by immunohistochemical analysis.

IP Image CBP antibody immunoprecipitates CBP protein in IP experiments.

ChIP Image CREBBP antibody immunoprecipitates CREBBP protein-DNA complex in ChIP experiments.

IHC-P Image CBP antibody [C3], C-term detects CBP protein at nucleus on human cervical carcinoma by immunohistochemical analysis.

IHC-P Image CBP antibody [C3], C-term detects CBP protein at nucleus on mouse muscle by immunohistochemical analysis.

IHC-P Image CBP antibody [C3], C-term detects CBP protein at nucleus on mouse fore brain by immunohistochemical analysis.

ICC/IF Image Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using CBP , antibody at 1:200 dilution.

11 images

1 reference

Independent Validation

1 validation

产品编号 ABIN2854987

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Datasheet as PDF

CTNNB1 抗体 (N-Term)

KO Validated CTNNB1 适用: 人 WB, IHC, IF, FACS, IHC (p), IP, ICC, IHC (fro), ChIP, IHC (wm), CUT&RUN 宿主: 兔 Polyclonal unconjugated

Cleavage Under Targets and Release Using Nuclease validation image for anti-Catenin (Cadherin-Associated Protein), beta 1, 88kDa (CTNNB1) (N-Term) antibody (ABIN2855042)

Cleavage Under Targets and Release Using Nuclease validation image for anti-Catenin (Cadherin-Associated Protein), beta 1, 88kDa (CTNNB1) (N-Term) antibody (ABIN2855042)

WB Image beta Catenin antibody [N1N2-2], N-term detects beta Catenin protein by western blot analysis.

WB Image Sample (30 ug of whole cell lysate) A: HeLa 7.

IHC-P Image beta Catenin antibody [N1N2-2], N-term detects beta Catenin protein at membrane on mouse urinary bladder by immunohistochemical analysis.

WB Image Sample (50 ug of whole cell lysate) A: mouse brain 7.

IHC-P Image beta Catenin antibody [N1N2-2] detects beta Catenin protein at cell membrane in mouse colon by immunohistochemical analysis.

ICC/IF Image Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed A431, using beta Catenin, antibody (Green) at 1:200 dilution.

WB Image beta Catenin antibody [N1N2-2], N-term detects CTNNB1 protein by Western blot analysis.

IP Image Immunoprecipitation of beta Catenin protein from HeLa whole cell extracts using 5 μg of beta Catenin antibody, Western blot analysis was performed using beta Catenin antibody, EasyBlot anti-Rabbit IgG was used as a secondary reagent.

IHC-P Image beta Catenin antibody [N1N2-2] detects beta Catenin protein at cell membrane in mouse colon by immunohistochemical analysis.

IHC-P Image Immunohistochemical analysis of paraffin-embedded U373 xenograft, using beta Catenin, antibody at 1:500 dilution.

Effects of MCME on migration-related proteins in CL1-0 and CL1-5 cells.

Expression analysis of Wnt pathway genes in human OS cellsWestern blot analysis of active β-catenin, total β-catenin and β-actin proteins in human OS cell lines (143B, Saos-2, SJSA-1 , U-2 OS, MG-63) with human breast cancer cell high-level-expression controls (MCF7, MDA-MB-231) and additional human mesenchymal stem cell low-level-expression controls (hMSC).

SGSM2 silencing affected T47D cell adhesion and cell migration.

Interaction between SGSM2 and E-cadherin was verified by co-immunoprecipitation and immunofluorescence staining assays.

16 images

8 references

Independent Validation

1 validation

产品编号 ABIN2855042

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Datasheet as PDF

HDAC1 抗体

KO Validated HDAC1 适用: 人 WB, IF, IHC (p), IP, ICC, ChIP, IHC (fro), IHC (wm) 宿主: 兔 Polyclonal unconjugated

Cleavage Under Targets and Release Using Nuclease validation image for anti-Histone Deacetylase 1 (HDAC1) antibody (ABIN2854776)

Cleavage Under Targets and Release Using Nuclease validation image for anti-Histone Deacetylase 1 (HDAC1) antibody (ABIN2854776)

WB Image HDAC1 antibody detects HDAC1 protein by western blot analysis.

Library profiles comparing fragment size distributions on an E-Gel EX 2% agarose gel (Thermo Fisher).

IHC-P Image Immunohistochemical analysis of paraffin-embedded human testis, using HDAC1, antibody(10 μg/ml).

IHC-P Image HDAC1 antibody detects HDAC1 protein at nucleus on mouse colon by immunohistochemical analysis.

ICC/IF Image HDAC1 antibody detects HDAC1 protein at nucleus by immunofluorescent analysis.

IHC-P Image HDAC1 antibody detects HDAC1 protein at cytoplasm and nucleus in rat brain by immunohistochemical analysis.

ChIP Image HDAC1 antibody immunoprecipitates HDAC1 protein-DNA in ChIP experiments.

IHC-P Image HDAC1 antibody detects HDAC1 protein at nucleus in human lung adenocarcinoma by immunohistochemical analysis.

IHC-P Image HDAC1 antibody detects HDAC1 protein at nucleus on mouse liver by immunohistochemical analysis.

ICC/IF Image Immunofluorescence analysis of paraformaldehyde-fixed A431, using HDAC1, antibody at 1:200 dilution.

IP Image HDAC1antibody immunoprecipitates HDAC1 protein in IP experiments.

HDAC1/2 depletion in adult Schwann cells leads to motor and sensory dysfunction in peripheral nerves.

HDAC1/2 depletion in adult Schwann cells leads to motor and sensory dysfunction in peripheral nerves.

SAHA affects the expression of suvivin and XIAP at the transcriptional level.

HDAC1/2 are robustly upregulated in SCs after lesion.

HDAC1/2 are robustly upregulated in SCs after lesion.

Overexpression of peptidase inhibitor 16 (PI16) attenuates angiotensin II (Ang II)-induced fibrotic gene expression and histone 3 modification in NRCFs.

The effects of peptidase inhibitor 16 (PI16) on newborn rat cardiac fibroblasts (NRCFs) are partially rescued by overexpression of HDAC1.

The effects of peptidase inhibitor 16 (PI16) on newborn rat cardiac fibroblasts (NRCFs) are partially rescued by overexpression of HDAC1.

Alignment tracks from CUT&RUN targeting HDAC1 in Mouse fore limb (11.

22 images

7 references

Independent Validation

1 validation

产品编号 ABIN2854776

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Datasheet as PDF

STAT3 抗体 (C-Term)

KO Validated STAT3 适用: 人 WB, IF, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated

Cleavage Under Targets and Release Using Nuclease validation image for anti-Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3) (C-Term) antibody (ABIN2855865)

Cleavage Under Targets and Release Using Nuclease validation image for anti-Signal Transducer and Activator of Transcription 3 (Acute-Phase Response Factor) (STAT3) (C-Term) antibody (ABIN2855865)

WB Image Sample (20 ug of whole cell lysate) A: human ESC 7.

WB Image Sample(30 ug whole cell lysate) A: 293T B: A431 , C: H1299 D: HeLa S3 , E: Hep G2 , F: MOLT4 , 7.

WB Image STAT3 antibody [C3], C-term detects STAT3 protein by Western blot analysis.

WB Image STAT3 antibody [C3], C-term detects STAT3 protein by Western blot analysis.

ICC/IF Image Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using STAT3, antibody at 1:200 dilution.

Effects of treatment with antrodan only, cisplatin only, and a combination of both on protein expression of MMP-2 and MMP-9, and on the phosphorylation and protein expression of signal transducer and activator of transcription 3 (STAT3), extracellular signal-regulated kinase 1 (ERK-1), c-Jun N-terminal kinase 1 (JNK1), JNK2, and p38 in lung tissues of C57BL/6 mice.

Effects of treatment with antrodan only, cisplatin only, and a combination of both on protein expression of MMP-2 and MMP-9, and on the phosphorylation and protein expression of STAT3, ERK1, JNK1, JNK2, and p38 in liver tissues of C57BL/6 mice.

MLN8237 treatment resulted in abnormal activation of Akt/Stat3 pathway.

10 images

1 reference

Independent Validation

1 validation

产品编号 ABIN2855865

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Datasheet as PDF

Calretinin 抗体

CALB2 适用: 人 WB, IHC (p), IF, ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated

Immunohistochemistry validation image for anti-Calbindin 2 (CALB2) antibody (ABIN2855596)

IHC-P Image Immunohistochemical analysis of paraffin-embedded Mouse retina, using Calretinin, antibody at 1:250 dilution.

WB Image Calretinin antibody detects CALB2 protein by Western blot analysis.

WB Image Sample (30 ug of whole cell lysate) A: A431 , 12% SDS PAGE Calretinin antibody antibody diluted at 1:1000

ICC/IF Image Immunofluorescence analysis of paraformaldehyde-fixed A431, using Calretinin , antibody at 1:200 dilution.

WB Image Sample (50 ug of whole cell lysate) A: mouse brain 12% SDS PAGE antibody diluted at 1:1000

IHC-P Image Calretinin antibody detects Calretinin protein at cytosol on mouse fore brain by immunohistochemical analysis.

7 images

1 reference

Independent Validation

1 validation

产品编号 ABIN2855596

获取报价

Datasheet as PDF

beta Amyloid 抗体 (N-Term)

Abeta 适用: 人, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated

Immunohistochemistry validation image for anti-Amyloid beta (Abeta) (N-Term) antibody (ABIN95037)

Western Blot of Rabbit anti-Beta Amyloid antibody.

Immunofluorescence microscopy of Rabbit Anti-Beta Amyloid antibody using HeLa cells fixed with MeOH.

Immunohistochemistry with anti-beta amyloid antibody showing amyloid beta plaque staining in human Alzheimer’s disease brain at 10x and 20x (B & C).

ELISA results of purified Rabbit anti-Beta Amyloid Antibody tested against BSA-conjugated peptide of immunizing peptide.

Human Heart (formalin-fixed, paraffin-embedded) stained with Anti-Beta Amyloid Antibody at 5 ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Western Blot of Rabbit anti-Beta Amyloid Antibody.

Immunofluorescence (IF) image for anti-Amyloid beta (Abeta) (N-Term) antibody (ABIN95037)

9 images

1 reference

Independent Validation

2 validations

产品编号 ABIN95037

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Datasheet as PDF

Collagen IV 抗体

COL4 适用: 人, Cow, 哺乳动物 WB, ELISA, IHC, IP 宿主: 兔 Polyclonal unconjugated

Immunohistochemistry validation image for anti-Collagen, Type IV (COL4) antibody (ABIN5596835)

Immunohistochemistry validation image for anti-Collagen, Type IV (COL4) antibody (ABIN5596835)

Immunohistochemistry validation image for anti-Collagen, Type IV (COL4) antibody (ABIN5596835)

anti collagen IV antibody (600-401-106 Lot 25440, 1:400, 45 min RT) showed strong staining in FFPE sections of human kidney (Left) with strong red staining observed in glomeruli; and liver (Right) with strong staining in sinusoids.

SMFC tracking in metamorphosed newt limb regeneration.

SMFC tracking in larval newt limb regeneration.

Lamina lucida and lamina densa regenerate before new ECM deposition.

SMFC tracking in larval newt limb regeneration.

SMFC tracking in metamorphosed newt limb regeneration.

SMFC tracking in larval newt limb regeneration.

SMFC tracking in larval newt limb regeneration.

SMFC tracking in metamorphosed newt limb regeneration.

12 images

8 references

Independent Validation

1 validation

产品编号 ABIN5596835

获取报价

Datasheet as PDF

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