MADCAM1 抗体 (Middle Region)
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北京 101111
Quick Overview for MADCAM1 抗体 (Middle Region) (ABIN3044076)
抗原
See all MADCAM1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- Middle Region
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原理
- Anti-MAdCAM1 Antibody
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序列
- EEPQGDEDVL FRVTERWR
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特异性
- No cross reactivity with other proteins.
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交叉反应 （详细）
- No cross-reactivity with other proteins
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产品特性
- Anti-MAdCAM1 Antibody. Tested in IF, IHC, WB applications. This antibody reacts with Human.
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纯化方法
- Immunogen affinity purified.
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免疫原
- A synthetic peptide corresponding to a sequence in the middle region of human MAdCAM1.
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亚型
- IgG
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anti-Mucosal Vascular Addressin Cell Adhesion Molecule 1 (MADCAM1) antibody
MADCAM1 适用: 人, 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Mucosal Vascular Addressin Cell Adhesion Molecule 1 (MADCAM1) (AA 151-250) antibodyMADCAM1 适用: 人, 小鼠 WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Mucosal Vascular Addressin Cell Adhesion Molecule 1 (MADCAM1) (AA 19-317) antibodyMADCAM1 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Mucosal Vascular Addressin Cell Adhesion Molecule 1 (MADCAM1) antibodyMADCAM1 适用: 人 WB, FACS 宿主: 兔 Monoclonal 24GB660 unconjugated Recombinant Antibody
anti-Mucosal Vascular Addressin Cell Adhesion Molecule 1 (MADCAM1) (AA 85-180) antibodyMADCAM1 适用: 小鼠 WB, ELISA, FACS, IF (cc), IF (p), IHC (p), IHC (fro), ICC 宿主: 兔 Polyclonal unconjugated
anti-Mucosal Vascular Addressin Cell Adhesion Molecule 1 (MADCAM1) (AA 29-297) antibodyMADCAM1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
- Western blot, 0.1-0.5 μg/mL, Human Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human Immunofluorescence, 5 μg/mL, Human
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说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and IHC(F).
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限制
- 仅限研究用
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- by
- Human Protein Atlas
- No.
- #300031
- 日期
- 2016.12.13
- 抗原
- MADCAM1
- Lot Number
- Method validated
- Immunohistochemistry
- Positive Control
- colon, spleen
- Negative Control
- Notes
- Passed. ABIN3044076 staining is consistent with RNA-seq data and published literature.
- Primary Antibody
- ABIN3044076
- Secondary Antibody
- HRP polymer (ThermoFisher Scientific, TL-125-PH)
- Full Protocol
- Sample preparation:
- Fixation and TMA preparation according to PMID: 22688270.
- Cut 4µm TMA sections using a waterfall microtome (ThermoFisher Scientific, HM355S).
- Dry sections ON at RT and then back them for 12-24h at 50°C.
- Deparaffinization and rehydration in xylene and graded alcohol series:
- xylene twice for 3min.
- 100% ethanol for 1min.
- 95% ethanol for 1min.
- 0.3% H2O2 in 95% ethanol for 5min to block endogenous peroxidase.
- 70% ethanol for 1min.
- 50% ethanol for 1min.
- distilled H2O for 2min.
- Antigen Retrieval by Heat Induced Epitope Retrieval (HIER):
- Boil the sections in Lab Vision Citrate buffer pH6.0 (ThermoFisher Scientific, TA-250-PM1X) for 4min at 125°C in a decloaking chamber (Biocare Medical).
- Allow slides to cool down to 90°C in the decloaking chamber.
- Immunostaining in a Lab Vision Autostainer 480 (ThermorFisher Scientific) at RT with 300µl of reagents for each step:
- Rinse sections in Lab Vision wash buffer with extra tween added to a final concentration of 0.2% (ThermoFisher Scientific, TA-999-TT and TA-125-TW) wash buffer.
- Block sections with Lab Vision Ultra V-Block (ThermoFisher Scientific, TA-125-UB) for 5min.
- Rinse sections 2x in wash buffer.
- Incubate sections with primary rabbit anti-MADCAM1 antibody (antibodies-online, ABIN3044076) diluted 1:900 for 30min.
- Rinse sections 3x in wash buffer.
- Incubate sections with labeled HRP polymer (ThermoFisher Scientific, TL-125-PH) for 30min.
- Rinse sections 2x in wash buffer.
- Develop in DAB Quanto solution (ThermoFisher Scientific, TL-125-QHDX) for 5min.
- Rinse sections in distilled H2O.
- Washing, counterstaining, and coverslipping in Autostainer XL (Leica Biosystems, ST5010) at RT:
- Counterstaining in Mayer’s hematoxylin plus (HistoLab, 01820) for 7.5min.
- Rinse sections in tap water for 5min.
- Rinse sections in lithium carbonate water diluted 1:5 for 5min.
- Dehydration in graded ethanol series and Neo-Clear (Merck Millipore, 109843) according to the manufacturer recommendations.
- Automated mounting (Leica Biosystems, CV5030) of coverslip with Pertex (Histolab, 00871).
- Microscopy:
- Image acquisition on a Leica Aperio AT2 and Aperio Scanscope AT at 20x magnification.
- Experimental Notes
- ABIN3044076 shows nice staining in a subset of vessels (endothelial cells), present in intestine and spleen.
生效 #300031 (Immunohistochemistry)!['独立验证'标志]( "成功验证")
!['独立验证'标志]( "成功验证")
Validation Images![Staining of human colon (A.) and spleen (B.) sections in a tissue microarray with ABIN3044076. See the protocol for detailed information.]()
Staining of human colon (A.) and spleen (B.) sections in a tissue microarray with ABIN3044076. See the protocol for detailed information.
Full Methods -
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles. -
有效期
- 12 months
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- MADCAM1 (Mucosal Vascular Addressin Cell Adhesion Molecule 1 (MADCAM1))
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别名
- MADCAM1
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背景
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Background: MADCAM1 (Mucosal Vascular Addressin Cell Adhesion Molecule 1), also known as MACAM1, is a protein that in humans is encoded by the MADCAM1 gene. By PCR-based analysis of somatic cell hybrids, Leung et al. (1997) mapped the MACAM1 gene to chromosome 19. The protein encoded by this gene is an endothelil cell adhesion molecule that interacts preferentially with the leukocyte beta7 integrin LPAM-1 (alpha4 / beta7), L-selectin, and VLA-4 (alpha4/beta1) on myeloid cells to leukocytes into mucosal and inflamed tissues. It is a member of the immunoglobulin superfamily and is similar to ICAM-1 and VCAM-1.
Gene Full Name: mucosal vascular addressin cell adhesion molecule 1
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分子量
- 60 kDa
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基因ID
- 8174
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UniProt
- Q13477
抗原
-
