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KCNQ4 抗体 (C-Term)

Name: KCNQ4 抗体 (C-Term)
Brand: antibodies-online
SKU: ABIN3185286
Availability: OutOfStock
Rating: 5 (1 reviews)

KCNQ4 适用: 人, 小鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated

Western Blotting validation image for anti-Potassium Voltage-Gated Channel, KQT-Like Subfamily, Member 4 (KCNQ4) (C-Term) antibody (ABIN3185286)

Western Blotting (WB) image for anti-Potassium Voltage-Gated Channel, KQT-Like Subfamily, Member 4 (KCNQ4) (C-Term) antibody (ABIN3185286)

2 images

(1 validation)

产品编号 ABIN3185286

发货至: 中国

Datasheet as PDF

抗原 See all KCNQ4 抗体

KCNQ4 (Potassium Voltage-Gated Channel, KQT-Like Subfamily, Member 4 (KCNQ4))
抗原表位
12

5

4

3

1

1

1

1

1

1

1

1

1

1
C-Term
适用
39

12

8

2

1

1

1

1

1

1

1

1
人, 小鼠
宿主
23

17

1
兔
Compatible Secondaries
克隆类型
24

17
多克隆
标记
21

3

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1
This KCNQ4 antibody is un-conjugated
应用范围
29

19

17

15

13

13

11

1

1

1

1

1

1
Western Blotting (WB), ELISA

特异性

KCNQ4 Polyclonal Antibody detects endogenous levels of KCNQ4 protein.

产品特性

Rabbit Polyclonal to KCNQ4.

纯化方法

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

免疫原

Synthesized peptide derived from the C-terminal region of human KCNQ4.

亚型

IgG
anti-Potassium Voltage-Gated Channel, KQT-Like Subfamily, Member 4 (KCNQ4) (AA 2-77) antibody
KCNQ4 适用: 人 WB, IHC, IP, IF, ICC, AA 宿主: 小鼠 Monoclonal N43-6 (Formerly S43-6) unconjugated

anti-Potassium Voltage-Gated Channel, KQT-Like Subfamily, Member 4 (KCNQ4) (AA 596-695) antibody
KCNQ4 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2H6 unconjugated

anti-Potassium Voltage-Gated Channel, KQT-Like Subfamily, Member 4 (KCNQ4) (AA 2-77) antibody (HRP)
KCNQ4 适用: 人 WB, IHC, IP, IF, ICC, AA 宿主: 小鼠 Monoclonal N43-6 (Formerly S43-6) HRP

anti-Potassium Voltage-Gated Channel, KQT-Like Subfamily, Member 4 (KCNQ4) (AA 2-77) antibody (FITC)
KCNQ4 适用: 人 WB, IHC, IP, IF, ICC, AA 宿主: 小鼠 Monoclonal N43-6 (Formerly S43-6) FITC

anti-Potassium Voltage-Gated Channel, KQT-Like Subfamily, Member 4 (KCNQ4) (AA 2-77) antibody (Alkaline Phosphatase (AP))
KCNQ4 适用: 人 WB, IHC, IP, IF, ICC, AA 宿主: 小鼠 Monoclonal N43-6 (Formerly S43-6) Alkaline Phosphatase (AP)

anti-Potassium Voltage-Gated Channel, KQT-Like Subfamily, Member 4 (KCNQ4) (AA 2-77) antibody (Biotin)
KCNQ4 适用: 人 WB, IHC, IP, IF, ICC, AA 宿主: 小鼠 Monoclonal N43-6 (Formerly S43-6) Biotin

anti-Potassium Voltage-Gated Channel, KQT-Like Subfamily, Member 4 (KCNQ4) (AA 2-77) antibody (PE)
KCNQ4 适用: 人 WB, IHC, IP, IF, ICC, AA 宿主: 小鼠 Monoclonal N43-6 (Formerly S43-6) PE

anti-Potassium Voltage-Gated Channel, KQT-Like Subfamily, Member 4 (KCNQ4) (AA 2-77) antibody (APC)
KCNQ4 适用: 人 WB, IHC, IP, IF, ICC, AA 宿主: 小鼠 Monoclonal N43-6 (Formerly S43-6) APC

anti-Potassium Voltage-Gated Channel, KQT-Like Subfamily, Member 4 (KCNQ4) (AA 2-77) antibody (PerCP)
KCNQ4 适用: 人 WB, IHC, IP, IF, ICC, AA 宿主: 小鼠 Monoclonal N43-6 (Formerly S43-6) PerCP

anti-Potassium Voltage-Gated Channel, KQT-Like Subfamily, Member 4 (KCNQ4) (AA 2-77) antibody (Atto 488)
KCNQ4 适用: 人 WB, IHC, IP, IF, ICC, AA 宿主: 小鼠 Monoclonal N43-6 (Formerly S43-6) Atto 488

应用备注

WB 1:500-1:2000, ELISA 1:5000,

限制

仅限研究用
生效 #102194 (Western Blotting)

by

Dipartimento di Medicina e Scienze della Salute, Università degli Studi del Molise, Campobasso

No.

#102194

日期

2019.04.11

抗原

Kv7.4/KCNQ4

Lot Number

3824901

Method validated

Western Blotting

Positive Control

NCHO cells non transfected or transfected with each other member of the Kv7 family (Kv7.1, Kv7.2, Kv7.3 or Kv7.5)

Negative Control

Western blot analysis of KCNQ4/KV7.4 using ABIN3185286. Total lysates from non-transfected (NT) CHO cells or cells transfected with plasmids expressing human Kv7.4 (Kv7.4) cDNAs were prepared and separated as described in the protocol. GAPDH served as loading control.

Notes

Passed. ABIN3185286 specifically detects KCNQ4/Kv7.4 in lysates from CHO cells expressing recombinant human KCNQ4/Kv7.4.

Validation Images

Western blot analysis of KCNQ4/KV7.4 using ABIN3185286. Total lysates from non-transfected (NT) CHO cells or cells transfected with plasmids expressing human Kv7.4 (Kv7.4) cDNAs were prepared and separated as described in the protocol. GAPDH served as loading control.
Full Methods
Primary Antibody

ABIN3185286

Secondary Antibody

goat anti-rabbit IgG (H+L) antibody (ImmunoReagents, GTXMU-003-DHRPX, lot 31-118-043013)

Full Protocol

Grow CHO cells (Chinese Hamster Ovary cells) in DMEM medium (Microgem, L0106-500) supplemented with 10% fetal bovine serum (Microgem, S1810-500) and 1x Penicillin-Streptomycin mix (Microgem, L0022-100), at 37°C and 5% CO₂ in 60mm plates.
Transfect CHO cells with plasmids expressing Kv7.4 with 5µg of plasmid each and 1µg of plasmid expressing EGFP as a transfection marker using Lipofectamine 2000 (invitrogen, 11668019) following the manufacturer´s instructions.
Lyse cells in an appropriate volume of B-buffer, containing 150mM NaCl, 10mM Tris-HCl pH7.4, 1mM EDTA, 1% SDS and 1x protease inhibitors (Roche, 11836145001, lot 11231400).
Determine total protein content of the lysates using a Bradford protein assay (AppliChem, A6932, lot 6J015116).
Denature 20µg of total protein for 10min at 100°C in 1/3 volume of 4x Laemmli SDS sample buffer and subsequently separate them on a denaturing 8% SDS-PAGE gel in Mini-PROTEAN Electrophoresis Cells (Bio-Rad) for 2-3h at 100V.
Transfer proteins onto PVDF filter (Bio-Rad, 1620177, lot 28369A01) with transfer buffer (25mM Tris, 192mM glycine, 20% methanol) in a western blotting system at 40mA at 4°C ON.
Block the membrane with blocking buffer (PBS, 5% milk dissolved, 1% Tween20) for 1h at RT.
Incubation with primary
rabbit anti-KCNQ4/Kv7.4 antibody (antibodies-online, ABIN3185286, 3824901) diluted 1:500 in blocking buffer ON at 4°C or
rabbit anti-GAPDH (abcam, ab1816002, lot GR217575-62) diluted 1:5000 in blocking buffer ON at 4°C.
Wash membrane 3x for 10min with PBS-T.
Incubation with secondary goat anti-rabbit IgG (H+L) antibody (ImmunoReagents, GTXMU-003-DHRPX, lot 31-118-043013) diluted 1:500 (for ABIN3185286) or 1:20000 (for anti-GAPDH) in blocking buffer for 1h at RT.
Wash membrane 3x for 10min with PBS-T.
Treat PVDF filters with chemiluminescence solutions (ECL, Promega) for 5min at RT then develop and acquire signals.

Experimental Notes
状态

Liquid

浓度

1 mg/mL

缓冲液

Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Avoid repeated freeze/thaw cycles.

储存条件

-20 °C

储存方法

Store at -20°C.
抗原

KCNQ4 (Potassium Voltage-Gated Channel, KQT-Like Subfamily, Member 4 (KCNQ4))

别名

KCNQ4 (KCNQ4 产品)

别名

KCNQ4 antibody, k(v)7.4 antibody, kv7.4 antibody, DFNA2 antibody, DFNA2A antibody, KV7.4 antibody, potassium voltage-gated channel subfamily Q member 4 antibody, potassium channel, voltage gated KQT-like subfamily Q, member 4 antibody, potassium voltage-gated channel, subfamily Q, member 4 antibody, KCNQ4 antibody, kcnq4 antibody, Kcnq4 antibody

分子量

80 kDa

基因ID

9132

UniProt

P56696

途径

Sensory Perception of Sound