ELF1 抗体 (C-Term)
(1 validation)-
- 抗原 See all ELF1 抗体
- ELF1 (E74-Like Factor 1 (Ets Domain Transcription Factor) (ELF1))
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抗原表位
- C-Term
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This ELF1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)
- 特异性
- ELF1 Antibody detects endogenous levels of total ELF1.
- 预测反应
- Horse
- 纯化方法
- The antiserum was purified by peptide affinity chromatography using SulfoLinkTM Coupling Resin (Thermo Fisher Scientific).
- 免疫原
- A synthesized peptide derived from human ELF1, corresponding to a region within C-terminal amino acids.
- 亚型
- IgG
- Top Product
- Discover our top product ELF1 Primary Antibody
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anti-E74-Like Factor 1 (Ets Domain Transcription Factor) (ELF1) (C-Term) antibody
Verified ELF1 适用: 人, 小鼠 WB, ELISA, IHC 宿主: 山羊 Polyclonal unconjugated
anti-E74-Like Factor 1 (Ets Domain Transcription Factor) (ELF1) (N-Term) antibodyELF1 适用: 人, 小鼠, 兔, 大鼠, 马, 犬 WB 宿主: 兔 Polyclonal unconjugated
anti-E74-Like Factor 1 (Ets Domain Transcription Factor) (ELF1) (AA 1-210) antibodyELF1 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-E74-Like Factor 1 (Ets Domain Transcription Factor) (ELF1) (AA 421-520) antibodyELF1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3B7 unconjugated
anti-E74-Like Factor 1 (Ets Domain Transcription Factor) (ELF1) (AA 1-619) antibodyELF1 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-E74-Like Factor 1 (Ets Domain Transcription Factor) (ELF1) (AA 142-191) antibodyELF1 适用: 人, 兔, 大鼠, 马, 犬, Cow, 豚鼠, Bat, Hamster, 猴, 非洲爪蟾 IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-E74-Like Factor 1 (Ets Domain Transcription Factor) (ELF1) (C-Term) antibodyELF1 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-E74-Like Factor 1 (Ets Domain Transcription Factor) (ELF1) (AA 1-619) antibodyELF1 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-E74-Like Factor 1 (Ets Domain Transcription Factor) (ELF1) (AA 540-589) antibodyELF1 适用: 人, 兔, 马 WB 宿主: 兔 Polyclonal unconjugated
anti-E74-Like Factor 1 (Ets Domain Transcription Factor) (ELF1) (AA 131-180) antibodyELF1 适用: 人, 小鼠, 兔, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- WB 1:500-1:2000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000
- 限制
- 仅限研究用
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- by
- Dittmann Lab, Microbiology Department, NYU Langone Health, NYU School of Medicine
- No.
- #104221
- 日期
- 2019.10.28
- 抗原
- ELF1
- Lot Number
- 4318850
- Method validated
- Western Blotting
- Positive Control
A549 (endogenous ELF1), ELF1 recombinant protein
- Negative Control
- Notes
Passed. ABIN6261511 recognizes recombinant and endogenous human ELF1. The antibody does also reveal an additional larger MW protein band in A549 cell lysates.
- Primary Antibody
- ABIN6261511
- Secondary Antibody
- goat anti-rabbit HRP-conjugated antibody (Invitrogen, G21234)
- Full Protocol
- Grow A549 cells (ATCC, CCL-185) in DMEM medium (Corning, 10-013-CV) supplemented with 10% FBS (Atlanta biologicals, S11150) and Penicillin-Streptomycin (Corning, 30-002-CI), at 37°C and 5% CO2 to 3x104 cells/cm2 in 2ml on a 6 well plate (Costar, 3516).
- Wash cells once with 1x PBS treat with trypsin (Corning, 25-053-CI).
- Take cells up in growth medium.
- Wash cells once with 1x PBS.
- Resuspend approximately 2.05x105cells/well in 50-100µl 1x LDS sample buffer (Life Technologies, B0007). As positive control, take 20µg recombinant ELF1 (Abnova, H00001997-P01) in 1x LDS sample buffer.
- Denature samples for 3min at 95°C and subsequently keep them on ice.
- Separate samples on a Bolt 4-12% Bis-Tris Plus Gel (Invitrogen, NW04122) in an electrophoresis chamber (Mini Gel Tank, Invitrogen, A25977) for 30min at 80V and then for 60min at 120V.
- Transfer proteins onto nitrocellulose membrane (Invitrogen, IB23002) using an iBlot 2 Gel Transfer Device (Invitrogen).
- Block the membrane with TBS containing 0.05% Tween (TBST) containing 5% skim milk for 1h at RT.
- Incubation with primary
- rabbit anti-ELF1 antibody (antibodies-online, ABIN6261511, 4318850) diluted 1:1000 in TBST containing 0.5% skim milk ON at 4°C.
- loading control mouse anti-beta actin antibody (Invitrogen, MA5-15739) diluted 1:5000 in TBST containing 0.5% skim milk ON at 4°C.
- Wash membrane 3x for 10min with TBST.
- Incubation with secondary
- goat anti-rabbit HRP-conjugated antibody (Invitrogen, G21234) diluted 1:10000 in TBST containing 0.5% skim milk for 1h at RT.
- goat anti-mouse HRP-conjugated antibody (Invitrogen, G21040) diluted 1:10000 in TBST containing 0.5% skim milk for 1h at RT.
- Wash membrane 3x for 10min with TBST.
- Reveal protein bands using SuperSignal West Dura Extended Duration Substrate (ThermoFisher Scientific, 34075) and a ChemiDoc MP Imaging System (Bio-Rad).
- Experimental Notes
ABIN6261511 reveals a protein with an apparent molecular weight of approximately 62kDa. The expected molecular weight for endogenous human ELF1 is approximately 67kDa. The antibody also reveals a recombinant GST-tagged protein at the expected molecular weight (theoretical MW 93.9kDa).
Other ELF1-antibody dilutions were tested, but a dilution of 1:2500 was found optimal in terms of minimal background and strength of signal.
生效 #104221 (Western Blotting)!['独立验证'标志]( "成功验证")
!['独立验证'标志]( "成功验证")
Validation Images![Detection of human ELF1 by western blot using ABIN6261511 diluted 1:1000. Samples: whole cell lysate from A549 cells and human ELF1 recombinant protein. Expected molecular weight: approximately 67kDa.]()
Detection of human ELF1 by western blot using ABIN6261511 diluted 1:1000. Samples: whole cell lysate from A549 cells and human ELF1 recombinant protein. Expected molecular weight: approximately 67kDa.
Full Methods -
- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- Store at -20 °C. Stable for 12 months from date of receipt.
- 有效期
- 12 months
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- 抗原
- ELF1 (E74-Like Factor 1 (Ets Domain Transcription Factor) (ELF1))
- 别名
- ELF1 (ELF1 产品)
- 别名
- zef1 antibody, fb05d07 antibody, wu:fb05d07 antibody, Elf-1 antibody, Sts1 antibody, mElf-1 antibody, p70 antibody, xelf1 antibody, elf-1 antibody, E74 like ETS transcription factor 1 antibody, E74-like ETS transcription factor 1 antibody, E74-like factor 1 antibody, E74 like ETS transcription factor 1 L homeolog antibody, E74 like ETS transcription factor 1 S homeolog antibody, ELF1 antibody, elf1 antibody, Elf1 antibody, elf1.L antibody, elf1.S antibody
- 背景
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Description: Transcription factor that activates the LYN and BLK promoters. Appears to be required for the T-cell-receptor-mediated trans activation of HIV-2 gene expression. Binds specifically to two purine-rich motifs in the HIV-2 enhancer.
Gene: ELF1
- 分子量
- 67kDa
- 基因ID
- 1997
- UniProt
- P32519
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