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SOD1 抗体 (AA 2-154)

Name: SOD1 抗体 (AA 2-154)
SKU: ABIN2913314
Availability: OutOfStock
Rating: 5 (2 reviews)

SOD1 适用: 人, 马 WB, IHC, IP, IF/ICC 宿主: 兔 Polyclonal unconjugated

Western Blotting validation image for anti-Superoxide Dismutase 1, Soluble (SOD1) (AA 2-154) antibody (ABIN3058918)

Immunocytochemistry validation image for anti-Superoxide Dismutase 1, Soluble (SOD1) (AA 2-154) antibody (ABIN3058918)

ICC

Western blot analysis of recombinant Horse SOD1.

Western blot analysis of (1) Rat Serum, (2) Bovine Liver Tissue, (3) Bovine Heart Tissue, (4) Bovine Brain Tissue and (5) Bovine Serum.

4 images

(1 reference)

(2 validations)

产品编号 ABIN2913314

发货至: 中国

Datasheet as PDF

抗原 See all SOD1 抗体

SOD1 (Superoxide Dismutase 1, Soluble (SOD1))
抗原表位
33

20

16

16

10

8

7

6

6

6

5

3

3

2

2

2

2

1

1

1

1

1

1

1

1

1

1
AA 2-154
适用
170

97

90

39

23

17

15

15

15

13

11

10

10

10

10

10

4

4

4

2

2

1

1

1

1

1

1

1

1

1
人, 马
宿主
189

26

6

4

2

2

1
兔
Compatible Secondaries
克隆类型
193

36
多克隆
标记
100

28

22

12

10

9

7

7

7

7

2

2

2

2

2

2

2

1

1

1

1

1

1

1
This SOD1 antibody is un-conjugated
应用范围
209

133

112

57

50

44

31

19

18

14

13

9

6

4

4

3

3

2

2

1

1

1
Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (fixed cells) (IF/ICC)

纯化方法

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

免疫原

SOD1 (Ala2-Pro154)

亚型

IgG

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anti-Superoxide Dismutase 1, Soluble (SOD1) (AA 2-154) antibody
SOD1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Superoxide Dismutase 1, Soluble (SOD1) (AA 2-154) antibody
SOD1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Superoxide Dismutase 1, Soluble (SOD1) (AA 2-153) antibody
SOD1 适用: 兔 WB, IHC, IP, ICC 宿主: 豚鼠 Polyclonal unconjugated

anti-Superoxide Dismutase 1, Soluble (SOD1) (AA 2-154) antibody
SOD1 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Superoxide Dismutase 1, Soluble (SOD1) (AA 2-153) antibody
SOD1 适用: Pig WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Superoxide Dismutase 1, Soluble (SOD1) antibody
SOD1 适用: 人, 小鼠 WB, ELISA, ICC, FACS 宿主: 小鼠 Monoclonal 6F5 unconjugated

应用备注

Optimal working dilution should be determined by the investigator.

限制

仅限研究用
生效 #100074 (Western Blotting)

by

Royal Veterinary College, London

No.

#100074

日期

2016.11.17

抗原

SOD-1

Lot Number

Method validated

Western Blotting

Positive Control

Horse Fibroblasts, human SH-SY5Y

Negative Control

Notes

ABIN3058918 successfully labels SOD-1 in lysates of equine fibroblast and human SH-SY5Y cells.

Validation Images

Total protein lysates from horse fibroblasts and human SH-SY5Y cells were separated were separated on a polyacrylamide gel under denaturing conditions. SOD-1 and TUBB protein bands were revealed as described in the protocol section.

Horse fibroblasts were grown in a monolayer and stained with ABIN3058918 (red) as desecribed in the protocol. DAPI (blue) was used to visualize nuclei.
Full Methods
Primary Antibody

ABIN3058918

Secondary Antibody

Goat anti-rabbit-IgG HRP-linked whole antibody (Dako, D0487)

Full Protocol

Seed 2x10⁵ horse fibroblasts in a T25 flask and grow for 48h at 5% CO₂ in supplemented with Dulbecco’s modified Eagle media (Sigma-Aldrich, D5546), with 10% heat-inactivated fetal calf serum (FCS) and 2mM (1%) L-glutamine and 1% penicillin (10IU/ml)/streptomycin (100µg/ml) to form a monolayer of 70-90%.
Human SH-SY5Y are cultured in identical conditions.
Wash cells once ice cold PBS.
Add 500µl chilled RIPA buffer (50mM Tris pH 7.5, 150mM NaCl, 1% NP40, 0.5% Na-deoxychlorate, 0.5% SDS, 1mM EDTA, 1mM EGTA, 1mM PMSF and 1x protease inhibitor) to each flask and incubate for 1min on ice.
Scrape each monolayer and transfer protein aggregates to a separate 1.5ml microcentrifuge tube.
Incubate for 20min on ice.
Centrifuge at 14000rpm for 20min at 4°C.
Remove the supernatant and determine the concentration of soluble proteins against a BSA protein standard using a DC protein assay (Biorad, 5000112).
Adjust protein samples to 0.3mg/ml total protein, using RIPA buffer and loading buffer.
After boiling for 5-10min, 1-10µg of each sample is separated on an Any kD Mini-PROTEAN TGX Stain-Free Precast Gel (Biorad, 4568123) at 100V for 90min.
Transfer proteins onto a PVDF blotting membrane (Hybond P, GE Healthcare Life Sciences, RPN303D) for 90min at 300mA.
Block the membrane with PBST containing 10% dry milk for 1h at RT.
Incubation with primary SOD-1 antibody (antibodies-online, ABIN3058918) diluted 1:1000 and a TUBB loading control antibody (abcam, ab6046) diluted 1:1000 in PBST containing 5% dry milk ON at 4°C.
Wash 5 times with PBST over 1 hour whilst shaking.
Incubation with secondary goat anti-rabbit-IgG horseradish peroxidase-linked whole antibody (Dako, D0487) diluted 1:5000 in PBST for 1h at RT.
Wash 5 times with PBST over 1 hour whilst shaking.
Blots were developed with Amersham ECL Prime Western Blotting Detection reagent (GE Healthcare Life Sciences, RPN2232) and visualized on a Kodak developer.

Experimental Notes

The same human cell lysates were also used for a different blot with an anti-human SOD-1 antibody (lab stock). A band of the same molecular weight as in the horse fibroblast lysates was revealed.
生效 #100075 (Immunocytochemistry)

by

Royal Veterinary College, London

No.

#100075

日期

2016.11.17

抗原

SOD-1

Lot Number

Method validated

Immunocytochemistry

Positive Control

Horse Fibroblasts

Negative Control

Notes

ABIN3058918 achieves SOD-1 staining in horse fibroblasts when grown in a monolayer.

Validation Images

Total protein lysates from horse fibroblasts and human SH-SY5Y cells were separated were separated on a polyacrylamide gel under denaturing conditions. SOD-1 and TUBB protein bands were revealed as described in the protocol section.

Horse fibroblasts were grown in a monolayer and stained with ABIN3058918 (red) as desecribed in the protocol. DAPI (blue) was used to visualize nuclei.
Full Methods
Primary Antibody

ABIN3058918

Secondary Antibody

anti-rabbit labeled with Alexa Fluor 594

Full Protocol

Horse fibroblasts are seeded onto glass coverslips pre-treated with 1:10 diluted Matrigel (BD Biosciences, 354234) at a density of 1x10⁵ cells per coverslip in a 24 well plate.
Incubate for 48h at 37°C in 5% CO₂.
Wash coverslips with PBS.
Fix coverslips with 4% paraformaldehyde (PFA) and 0.1% triton for 20min at 4°C, followed by 8% PFA for 30min at RT.
Permeabilize cells with 0.5% triton for 30min at RT.
Wash coverslips 3 times with PBS.
Incubate coverslips with primary rabbit anti SOD-1 antibody (antibodies-online, ABIN3058918) diluted 1:40 in a dark, humidified chamber for 1h at RT. A negative control was incubated without primary antibody.
Wash coverslips 3 times with PBS.
Incubate with secondary goat anti rabbit labeled with Alexa Fluor 594 (ThermoFisher Scientific, A10239) diluted 1:500 for 1h at RT.
Wash coverslips 3 times with PBS.
Mount coverslips in a hard setting mount with VECTASHIELD HardSet Antifade Mounting Medium with DAPI (Vector Laboratories, H-1500).
Fluorescence microscopy is then used to image each coverslip.

Experimental Notes
状态

Liquid

浓度

500 μg/mL

缓冲液

0.01M PBS,  pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store at 4 °C for frequent use. Aliquot and store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Kronstein-Wiedemann, Stadtmüller, Traikov, Georgi, Teichert, Yosef, Wallenborn, Karl, Schütze, Wagner, El-Armouche, Tonn: "SARS-CoV-2 Infects Red Blood Cell Progenitors and Dysregulates Hemoglobin and Iron Metabolism." in: Stem cell reviews and reports, (2022) (PubMed).
抗原

SOD1 (Superoxide Dismutase 1, Soluble (SOD1))

别名

Superoxide Dismutase 1 (SOD1 产品)

别名

LOC692639 antibody, ALS antibody, ALS1 antibody, IPOA antibody, SOD antibody, hSod1 antibody, homodimer antibody, CG11793 antibody, Cu antibody, Cu-Zn SOD antibody, Cu/Zn SOD antibody, Cu/Zn sod antibody, Cu/Zn superoxide dismutase antibody, Cu/ZnSOD antibody, CuSOD antibody, CuZn SOD antibody, CuZn-SOD antibody, CuZn-SOD1 antibody, CuZnSOD antibody, Cu[2+]/Zn[2+]SOD antibody, Dmel\\CG11793 antibody, G antibody, Mn SOD antibody, SOD-1 antibody, SOD1 antibody, Sod-1 antibody, Sod1 antibody, To antibody, To-1 antibody, Zn SOD antibody, Zn Sod antibody, Zn-SOD antibody, ZnSod antibody, cSOD antibody, cSod antibody, dSOD1 antibody, l(3)108 antibody, l(3)68Af' antibody, l(3)G antibody, sod antibody, sod1 antibody, CU/ZN-SOD antibody, SODC antibody, DKFZP469M1833 antibody, B430204E11Rik antibody, Cu/Zn-SOD antibody, Ipo-1 antibody, Ipo1 antibody, SOD1L1 antibody, XSODB antibody, als antibody, als1 antibody, ipoa antibody, sod1-a antibody, ZSOD antibody, cuzn antibody, Cu/Zn superoxide dismutase antibody, superoxide dismutase 1 antibody, Superoxide dismutase 1 antibody, superoxide dismutase 1, soluble antibody, superoxide dismutase 1 S homeolog antibody, Superoxide dismutase [Cu-Zn] antibody, superoxide dismutase 1 L homeolog antibody, superoxide dismutase [Cu-Zn]-like antibody, superoxide dismutase [Cu-Zn] antibody, superoxide dismutase Sod1 antibody, SOD antibody, SOD1 antibody, Sod1 antibody, sod1 antibody, sod1.S antibody, sod-1 antibody, sod1.L antibody, LOC101451855 antibody, LOC101115136 antibody

途径

Sensory Perception of Sound, Transition Metal Ion Homeostasis