anti-人 E-cadherin 抗体 for BioImaging

Recommended E-cadherin Antibody (supplied by: Log in to see )

抗原
Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) 抗体
  • Arc-1
  • CD324
  • CDHE
  • ECAD
  • LCAM
  • UVO
  • E-cadherin
  • uvo
  • lcam
  • E-Cad
  • XBcad
  • l-cam
  • xcdh1
  • cdhc-A
  • xb-cad
  • XTCAD-1
  • c-cadherin
  • cadherin-1
  • uvomorulin
  • XB-cadherin
  • arc-1
  • cd324
  • cdhe
  • ecad
  • AA960649
  • E-cad
  • Ecad
  • L-CAM
  • Um
  • cadherin 1
  • cadherin 1, type 1, E-cadherin (epithelial)
  • cadherin 1, type 1 S homeolog
  • CDH1
  • cdh1
  • Cdh1
  • cdh1.S
适用

797
239
144
40
26
16
9
8
8
7
6
4
3
1
1
1
1
1
1
1
1
宿主
小鼠
542
265
37
22
2
克隆类型
单克隆
标记
This E-cadherin antibody is un-conjugated
43
39
34
31
24
21
21
17
17
15
13
13
13
13
12
12
11
10
8
7
7
6
4
3
3
3
3
3
3
2
2
应用范围
BioImaging (BI), Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB)
645
370
275
213
193
176
157
87
81
38
19
14
13
12
10
7
6
6
4
2
2
2
2
2
1
1
选项
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产品编号 ABIN2689191
产品在该地区无法提供。

General

抗原 Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) 抗体
适用
(797), (239), (144), (40), (26), (16), (9), (8), (8), (7), (6), (4), (3), (1), (1), (1), (1), (1), (1), (1), (1)
宿主 小鼠
(542), (265), (37), (22), (2)
克隆类型 (克隆位点)
单克隆   ( )
标记 This E-cadherin antibody is un-conjugated
(43), (39), (34), (31), (24), (21), (21), (17), (17), (15), (13), (13), (13), (13), (12), (12), (11), (10), (8), (7), (7), (6), (4), (3), (3), (3), (3), (3), (3), (2), (2)
应用范围 BioImaging (BI), Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB)
(645), (370), (275), (213), (193), (176), (157), (87), (81), (38), (19), (14), (13), (12), (10), (7), (6), (6), (4), (2), (2), (2), (2), (2), (1), (1)
Pubmed 3个引用可参考
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E-cadherin抗体详情

E-cadherin目标详情 使用细节 贮存及处理 References for anti-E-cadherin antibody (ABIN2689191) 图像
品牌 BD Pharmingen™
产品特性 The 67A4 monoclonal antibody recognizes the extracellular domain of human E-Cadherin (CD324). E-Cadherin is a 120- kDa transmembrane glycoprotein that is localized in the adherens junctions of epithelial cells. There it interacts with the cytoskeleton through the associated cytoplasmic catenin proteins. In addition to being a calcium-dependent adhesion molecule, E-Cadherin is also a critical regulator of epithelial junction formation. Its association with catenins is necessary for cell-to-cell adhesion. These E-Cadherin/catenin complexes associate with cortical actin bundles at both the zonula adherens and the lateral adhesion plaques. Tyrosine phosphorylation can disrupt these complexes, leading to changes in cell adhesion properties. E-Cadherin expression is often down-regulated in highly invasive, poorly differentiated carcinomas. Increased expression of E-Cadherin in these cells reduces their invasiveness. Thus, loss of expression or function of E-Cadherin appears to be an important step in tumorigenic progression. Pluripotent stem cells express E-Cadherin. Upon differentiation, an epithelial to mesenchymal transition results in the loss of E-cadherin expression and a gain in the expression of N-cadherin. Western blot analysis of CD324 (E-cadherin) expression in human breast adenocarcinoma and human embryonic stem (ES) cells. Cell Lysates from a human breast adenocarcinoma cell line MCF-7 (ATCC HTB-22, left blot) and H9 human ES Cells (WiCell, Madison, WI, right blot) were probed with Purified Mouse Anti-Human CD324 (E-Cadherin) monoclonal antibody at titrations of 0.125 (lanes 1), 0.063 (lanes 2), 0.032 (lanes 3) μg/mL. Proteins were detected using HRP Goat Anti-Mouse Ig (Cat. No. 554002). CD324 (E-cadherin) is identified as a band of ~120 kDa in MCF7 and Human ES cells. Immunoflourescent staining of CD324 (E-cadherin) on human embryonic stem (ES) cells. H9 human ES cells (WiCell, Madison, WI) passage 45 grown in mTESR™1 media (StemCell Technologies) on BD Matrigel™ hESC-qualified Matrix (Cat. No. 354277) were fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655). Cells were stained with Purified Mouse Anti-Human CD324 (E-Cadherin) monoclonal antibody (pseudo-colored green) at 2.5 μg/mL. The second-step reagent was Alexa Fluor® 488 goat anti-mouse Ig (Life Technologies), and cell nuclei were stained with DAPI (pseudo-colored blue). The images were captured on a BD Pathway™ 435 Cell Analyzer and merged using BD Attovision™ software.

BD Pharmingen™ Purified Mouse Anti-Human CD324 (E-Cadherin) - Purified - Clone 67A4 - Isotype Mouse IgG1, κ - Reactivity Hu - 50 μg
纯化方法 The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
免疫原 Human Breast Tumor Cell Line
克隆位点 67A4
亚型 IgG1, kappa

E-cadherin目标详情

E-cadherin抗体详情 使用细节 贮存及处理 References for anti-E-cadherin antibody (ABIN2689191) 图像 回页首
抗原
别名 CD324 E-Cadherin (CDH1 Antibody 摘要)
背景 Synonyms: E-cadherin
分子量 ~120 kDa
途径 Sensory Perception of Sound, Cell-Cell Junction Organization, Tube Formation

使用细节

E-cadherin抗体详情 E-cadherin目标详情 贮存及处理 References for anti-E-cadherin antibody (ABIN2689191) 图像 回页首
应用备注 Optimal working dilution should be determined by the investigator.
限制 仅限研究用

贮存及处理

E-cadherin抗体详情 E-cadherin目标详情 使用细节 References for anti-E-cadherin antibody (ABIN2689191) 图像 回页首
浓度 0.5 mg/mL
缓冲液 Aqueous buffered solution containing ≤0.09 % sodium azide.
储存液 Sodium azide
注意事项 This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件 4 °C
储存方法 Store undiluted at 4°C.

References for anti-E-cadherin antibody (ABIN2689191)

E-cadherin抗体详情 E-cadherin目标详情 使用细节 贮存及处理 图像 回页首
有引用在:

DAmour, Agulnick, Eliazer, Kelly, Kroon, Baetge: "Efficient differentiation of human embryonic stem cells to definitive endoderm." in: Nature biotechnology, Vol. 23, Issue 12, pp. 1534-41, 2005

Cepek, Shaw, Parker, Russell, Morrow, Rimm, Brenner: "Adhesion between epithelial cells and T lymphocytes mediated by E-cadherin and the alpha E beta 7 integrin." in: Nature, Vol. 372, Issue 6502, pp. 190-3, 1994

Takeichi: "The cadherins: cell-cell adhesion molecules controlling animal morphogenesis." in: Development (Cambridge, England), Vol. 102, Issue 4, pp. 639-55, 1988