IL-1 beta ELISA 试剂盒
(1 reference)-
- 抗原 See all IL-1 beta (IL1B) ELISA试剂盒
- IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))
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适用
- 兔
- 检测方法
- Colorimetric
- 实验类型
- Sandwich ELISA
- 检测范围
- 45-12.000 pg/mL
- 最低检测浓度
- 45 pg/mL
- 应用范围
- ELISA
- 原理
- Rabbit IL-1 beta (IL-1 F2) ELISA Kit for cell culture supernatants, plasma, and serum samples.
- 样品类型
- Plasma, Cell Culture Supernatant, Serum
- Analytical Method
- Quantitative
- 特异性
- Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: rabbit IL-1alpha, IL-8, IL-17A, IL-21, Leptin, MIP-1 beta, MMP-9, NCAM-1, TNF alpha
- 灵敏度
- 45 pg/mL
- 产品特性
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- 组件
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- 试剂未包括
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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Interleukin 1, beta (IL1B) ELISA Kit
IL1B 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 1, beta (IL1B) ELISA KitIL1B 适用: 人 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 1, beta (IL1B) ELISA KitIL1B 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 1, beta (IL1B) ELISA KitIL1B 适用: 人 AA 117-269 Colorimetric Sandwich ELISA 3.9-250 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
Interleukin 1, beta (IL1B) ELISA KitIL1B 适用: 大鼠 AA 117-268 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Interleukin 1, beta (IL1B) ELISA KitIL1B 适用: 小鼠 AA 118-269 Colorimetric Sandwich ELISA 12.5-800 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Interleukin 1, beta (IL1B) ELISA KitIL1B 适用: 兔 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 1, beta (IL1B) ELISA KitIL1B 适用: 人 Colorimetric Sandwich ELISA 125 pg/mL - 8000 pg/mL Cell Culture Supernatant, Cerebrospinal Fluid, Serum, Urine
Interleukin 1, beta (IL1B) ELISA KitIL1B 适用: Pig Colorimetric Sandwich ELISA 1.56 pg/mL - 100 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 1, beta (IL1B) ELISA KitIL1B 适用: 小鸡 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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- 应用备注
- Recommended Dilution for serum and plasma samples2 fold
- 样本量
- 100 μL
- 板类型
- Pre-coated
- 实验流程
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- 实验流程
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Prepare all reagents, samples and standards as instructed in the manual.Add 100 μL of standard or sample to each well.Incubate 2.5 h at RT or O/N at 4 °C.Add 100 μL of prepared biotin antibody to each well.Incubate 1 h at RT.Add 100 μL of prepared Streptavidin solution to each well.Incubate 45 min at RT.Add 100 μL of TMB One-Step Substrate Reagent to each well.Incubate 30 min at RT.Add 50 μL of Stop Solution to each well.Read at 450 nm immediately.
- 限制
- 仅限研究用
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- 储存条件
- -20 °C
- 储存方法
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- 有效期
- 6 months
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: "The differential effects of leukocyte-containing and pure platelet-rich plasma (PRP) on tendon stem/progenitor cells - implications of PRP application for the clinical treatment of tendon injuries." in: Stem cell research & therapy, Vol. 6, pp. 173, (2015) (PubMed).
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: "The differential effects of leukocyte-containing and pure platelet-rich plasma (PRP) on tendon stem/progenitor cells - implications of PRP application for the clinical treatment of tendon injuries." in: Stem cell research & therapy, Vol. 6, pp. 173, (2015) (PubMed).
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- 抗原 See all IL-1 beta (IL1B) ELISA试剂盒
- IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))
- 别名
- IL-1 beta (IL1B 产品)
- 别名
- IL-1 ELISA Kit, IL1-BETA ELISA Kit, IL1F2 ELISA Kit, IL-1BETA ELISA Kit, IL1beta ELISA Kit, il1-b ELISA Kit, zgc:111873 ELISA Kit, IL-1B ELISA Kit, IL-1beta ELISA Kit, Il-1b ELISA Kit, IL1B ELISA Kit, IL-1 beta ELISA Kit, IL-1b ELISA Kit, interleukin 1 beta ELISA Kit, interleukin 1, beta ELISA Kit, IL1B ELISA Kit, il1b ELISA Kit, Il1b ELISA Kit
- 背景
- Interleukin-1 beta (IL-1 beta) (Lymphocyte proliferation-potentiating factor)
- 基因ID
- 100008990
- UniProt
- P14628
- 途径
- NF-kappaB Signaling, Interferon-gamma Pathway, TLR signaling, Negative Regulation of Hormone Secretion, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Autophagy, Cancer Immune Checkpoints, Inflammasome
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