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IL-1 beta ELISA 试剂盒

Name: IL-1 beta ELISA 试剂盒
SKU: ABIN411294
Availability: OutOfStock

The 小鼠 IL-1 beta ELISA Kit (ABIN411294) is a Colorimetric ELISA Kit designed to quantify 小鼠 IL-1 beta. This ELISA Kit has been cited in 59 publications.

ELISA

1 image

(59 references)

产品编号 ABIN411294

发货至: 中国

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Quick Overview for IL-1 beta ELISA 试剂盒 (ABIN411294)

抗原

See all IL-1 beta (IL1B) ELISA试剂盒

IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

抗原表位

AA 118-269

适用

小鼠

检测方法

Colorimetric

实验类型

Sandwich ELISA

检测范围

12.5 pg/mL - 800 pg/mL

应用范围

ELISA

样品类型

Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum

Discover our top product IL1B ELISA Kit

最低检测浓度

12.5 pg/mL

原理

Sandwich Mouse IL-1 Beta/IL-1F2/IL1B ELISA Kit to quantitate Mouse IL1B in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Analytical Method

Quantitative

特异性

Expression system for standard: E.coli
Immunogen sequence: V118-S269
Capture antibody and Detection antibody: polyclonal antibody from goat, polyclonal antibody from goat

交叉反应（详细）

There is no detectable cross-reactivity with other relevant proteins.

灵敏度

< 1 pg/mL

组件
1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers
试剂未包括

Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.
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Interleukin 1, beta (IL1B) ELISA Kit
IL1B 适用: 小鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Interleukin 1, beta (IL1B) ELISA Kit
IL1B 适用: 人 AA 117-269 Colorimetric Sandwich ELISA 3.9 pg/mL - 250 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum

Interleukin 1, beta (IL1B) ELISA Kit
IL1B 适用: 大鼠 AA 117-268 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum

Interleukin 1, beta (IL1B) ELISA Kit
IL1B 适用: 兔 Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Interleukin 1, beta (IL1B) ELISA Kit
IL1B 适用: 大鼠 Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Interleukin 1, beta (IL1B) ELISA Kit
IL1B 适用: 人 Colorimetric Sandwich ELISA 125 pg/mL - 8000 pg/mL Cell Culture Supernatant, Cerebrospinal Fluid, Serum, Urine

Interleukin 1, beta (IL1B) ELISA Kit
IL1B 适用: 鱼 Colorimetric Sandwich ELISA 3.12 pg/mL - 200 pg/mL Plasma, Serum, Tissue Homogenate

Interleukin 1, beta (IL1B) ELISA Kit
IL1B 适用: 人 Colorimetric Sandwich ELISA 3.906 pg/mL - 250 pg/mL Plasma, Serum, Tissue Homogenate

应用备注

Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

样本量

100 μL

板类型

Pre-coated

实验流程

ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

实验精密度

Intra-Assay Precision (CV%): 5.3%, 4.9%, 7.1%
Inter-Assay Precision (CV%): 6.1%, 6.8%, 7.4%

限制

仅限研究用
注意事项

Avoid multiple freeze-thaw cycles.

储存条件

-20 °C,4 °C

储存方法

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

有效期

12 months
Cai, Chen, Zhong, Zhang, Jiang, Xu, Shi: "Synergistic Effect of Compounds from a Chinese Herb: Compatibility and Dose Optimization of Compounds from N-Butanol Extract of Ipomoea stolonifera." in: Scientific reports, Vol. 6, pp. 27014, (2018) (PubMed).

Pang, Wu, Peng, Yang, Kuai, Qin, Cao, Sun, Chen, Vitek, Jiang: "Potential implications of Apolipoprotein E in early brain injury after experimental subarachnoid hemorrhage: Involvement in the modulation of blood-brain barrier integrity." in: Oncotarget, Vol. 7, Issue 35, pp. 56030-56044, (2018) (PubMed).

Gao, Huang, Zhao, Hu, Li, Guo, Zhao, Lu: "LL202 protects against dextran sulfate sodium-induced experimental colitis in mice by inhibiting MAPK/AP-1 signaling." in: Oncotarget, Vol. 7, Issue 39, pp. 63981-63994, (2018) (PubMed).

Liu, Zhang, Wu, Gong: "Foxo3a-dependent Bim transcription protects mice from a high fat diet via inhibition of activation of the NLRP3 inflammasome by facilitating autophagy flux in Kupffer cells." in: Oncotarget, Vol. 8, Issue 21, pp. 34258-34267, (2018) (PubMed).

Hu, Shen, Wang, Zhao: "Liver X Receptor Agonist TO901317 Attenuates Paraquat-Induced Acute Lung Injury through Inhibition of NF-κB and JNK/p38 MAPK Signal Pathways." in: BioMed research international, Vol. 2017, pp. 4652695, (2018) (PubMed).

Zhang, Wu, Xu, He, Yang, Li, Li, Ao, Cheng, Jia: "The cystathionine β-synthase/hydrogen sulfide pathway contributes to microglia-mediated neuroinflammation following cerebral ischemia." in: Brain, behavior, and immunity, Vol. 66, pp. 332-346, (2018) (PubMed).

Feng, Wei, Wang, Fu, Shi, Qu, Fan: "Rhein ameliorates adenomyosis by inhibiting NF-κB and β-Catenin signaling pathway." in: Biomedicine & pharmacotherapy, Vol. 94, pp. 231-237, (2018) (PubMed).

Wang, Dong, Wang, Liu, Sun, Li, Lin, Geng, Jin, Li: "Adolescent escitalopram prevents the effects of maternal separation on depression- and anxiety-like behaviours and regulates the levels of inflammatory cytokines in adult male mice." in: International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience, Vol. 62, pp. 37-45, (2018) (PubMed).

Li, Diao, Guo, Huang, Yang, Feng, Yan, Ning, Zheng, Chen, Wu: "VSIG4 inhibits proinflammatory macrophage activation by reprogramming mitochondrial pyruvate metabolism." in: Nature communications, Vol. 8, Issue 1, pp. 1322, (2018) (PubMed).

Du, Chen, Wang, Sun: "Pathway analysis of global gene expression change in dendritic cells induced by the polysaccharide from the roots of Actinidia eriantha." in: Journal of ethnopharmacology, Vol. 214, pp. 141-152, (2018) (PubMed).

Wu, Zhang, Qi, Zhang, Li, Li, Lin, Bai, Liu, Chen, Yang, Xu, Zhang, Yang: "Nicotine promotes atherosclerosis via ROS-NLRP3-mediated endothelial cell pyroptosis." in: Cell death & disease, Vol. 9, Issue 2, pp. 171, (2018) (PubMed).

Zhang, Li, Xu, Xiang, Ma: "Luteolin alleviates NLRP3 inflammasome activation and directs macrophage polarization in lipopolysaccharide-stimulated RAW264.7 cells." in: American journal of translational research, Vol. 10, Issue 1, pp. 265-273, (2018) (PubMed).

Zhang, Feng, Cui, Yang, Zhu et al.: "Pre-B cell leukemia transcription factor 3 induces inflammatory responses in human umbilical vein endothelial cells and murine sepsis via acting a competing endogenous RNA for high mobility group box ..." in: Molecular medicine reports, Vol. 17, Issue 4, pp. 5805-5813, (2018) (PubMed).

Lu, Zhang, Zhang, Zhou, Gao, Liu, Wang, Wu, Li, Hang: "Peroxiredoxin 2 activates microglia by interacting with Toll-like receptor 4 after subarachnoid hemorrhage." in: Journal of neuroinflammation, Vol. 15, Issue 1, pp. 87, (2018) (PubMed).

Xu, Liang, Li, Ma: "Signaling via the CXCR5/ERK pathway is mediated by CXCL13 in mice with breast cancer." in: Oncology letters, Vol. 15, Issue 6, pp. 9293-9298, (2018) (PubMed).

Xianchu, Ming, Xiangbin, Lan: "Grape seed proanthocyanidin extract supplementation affects exhaustive exercise-induced fatigue in mice." in: Food & nutrition research, Vol. 62, (2018) (PubMed).

Song, Pei, Hu, Pan, Xiong, Liu, Wu, Shang, Yao: "Death-associated protein kinase 1 mediates interleukin-1β production through regulating inlfammasome activation in Bv2 microglial cells and mice." in: Scientific reports, Vol. 8, Issue 1, pp. 9930, (2018) (PubMed).

Zhang, Li, Liu, Chen, Gong: "Activation of imidazoline I1 receptor by moxonidine regulates the progression of liver fibrosis in the Nrf2-dependent pathway." in: Biomedicine & pharmacotherapy, Vol. 90, pp. 821-834, (2017) (PubMed).

Yang, Dong, Hu, Chen, Fu, Wang, Xu, Tu: "Liver Kinase B1/AMP-Activated Protein Kinase Pathway Activation Attenuated the Progression of Endotoxemia in the Diabetic Mice." in: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Vol. 42, Issue 2, pp. 761-779, (2017) (PubMed).

Jin, Liu, Chen, Xiang, Ding, Li: "Effect of miR-146 targeted HDMCP up-regulation in the pathogenesis of nonalcoholic steatohepatitis." in: PLoS ONE, Vol. 12, Issue 3, pp. e0174218, (2017) (PubMed).

show more references
抗原 See all IL-1 beta (IL1B) ELISA试剂盒

IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

别名

IL1B

背景

Background: Interleukin-1beta (IL-1beta) is a potent stimulator of bone resorption whose gene is mapped to 2q14, and has been implicated in the pathogenesis of high bone turnover and osteoporosis. IL-1beta, a prominent microglia-derived cytokine, caused oligodendrocyte death in coculture with astrocytes and microglia, but not in pure culture of oligodendrocytes alone1. It also can cause nuclear export of a specific NCOR corepressor complex, resulting in derepression of a specific subset of nuclear factor-kappa-B (NFKB)-regulated genes2. Furthermore, Microenvironmental IL-1beta and, to a lesser extent, IL-1alpha are required for in vivo angiogenesis and invasiveness of different tumor cells3. Additional, the cooperation of IL-1beta and PDGFB induces contractile-to-synthetic phenotype modulation of human aortic smooth muscle cells in culture4. Moreover, the association with disease may be explained by the biologic properties of IL-1beta, which is an important proinflammatory cytokine and a powerful inhibitor of gastric acid secretion.
Gene Full Name: interleukin 1 beta

基因ID

16176

UniProt

P10749

途径

NF-kappaB Signaling, Interferon-gamma Pathway, TLR signaling, Negative Regulation of Hormone Secretion, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Autophagy, Cancer Immune Checkpoints, Inflammasome