IL-1 beta 抗体 (N-Term)
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- 抗原 See all IL-1 beta (IL1B) 抗体
- IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))
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抗原表位
- N-Term
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适用
- 人, 犬, 灵长类
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This IL-1 beta antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Radioimmunoassay (RIA), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 特异性
- Recombinant 153 aa human IL-1b produced in E. coli. The MW of the recombinant IL-1b was 17000 with the n-terminal amino acid at position alanine 117. This is the cleavage site generated by the IL-1b converting enzyme (ICE, caspase-1).
- 纯化方法
- Purified IgG
- 免疫原
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Recombinant human IL1B / IL1 Beta.
Type of Immunogen: Recombinant protein - Top Product
- Discover our top product IL1B Primary Antibody
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anti-Interleukin 1, beta (IL1B) (AA 101-200) antibody
IL1B 适用: 人, 小鼠, 大鼠, Cow, 兔, Pig WB, ELISA, FACS, IHC (p), IF (p), IF (cc), IHC (fro), DB 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 1, beta (IL1B) (AA 161-269) antibodyIL1B 适用: 人, 小鼠, 大鼠, Cow, 兔 WB, ELISA, IHC (p), IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 1, beta (IL1B) antibodyIL1B 适用: 人 WB, ELISA, IHC, ICC 宿主: 小鼠 Monoclonal 3A6 unconjugated
anti-Interleukin 1, beta (IL1B) (AA 170-269) antibodyIL1B 适用: 人 WB, ELISA, IP, IF 宿主: 小鼠 Monoclonal 2A8 unconjugated
anti-Interleukin 1, beta (IL1B) (AA 117-269) antibodyIL1B 适用: 人 WB, ELISA, ICC, FACS 宿主: 小鼠 Monoclonal 5C3B7 unconjugated
anti-Interleukin 1, beta (IL1B) (AA 117-269) antibodyIL1B 适用: 人 WB, ELISA, IHC, ICC, FACS 宿主: 小鼠 Monoclonal 6F12D5 unconjugated
anti-Interleukin 1, beta (IL1B) (AA 1-266) antibodyIL1B 适用: 绵羊 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 1, beta (IL1B) antibodyIL1B 适用: 人, 小鼠, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 1, beta (IL1B) (AA 1-269) antibodyIL1B 适用: 人 WB, PLA 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 1, beta (IL1B) antibodyIL1B 适用: 小鼠, 大鼠 WB, ELISA, IHC, IP, FACS, Neut, RIA 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Approved: ELISA (1:500 - 1:2000), IHC, IHC-Fr (1:100 - 1:200), IHC-P (1:100 - 1:200), IP (1:400 - 1:800), RIA (1:5000 - 1:10000), WB (1:100 - 1:200)
Usage: This IgG fraction antibody of anti- Human IL-1beta has been tested for use in neutralizations, ELISA, radioimmunoassays, immunohistochemistry, immunoblotting and immunoprecipitation. It recognizes the 17000 MW mature IL-10. For immunoblots, typically, IL-1beta is detected from supernatants or lysates of 2 x 106 endotoxin-stimulated human peripheral blood mononuclear cells (PBMC). PBMC are stimulated for 24 hours with 1 % (v/v) human serum plus 10 ng/mL E. coli LPS. For immunoprecipitation a dilution of 1:400 to 1:800 is suggested. Pre-clearing the preparation with a non-specific rabbit IgG (011-001-297) to reduce background. For immunohistochemistry a dilution of 1:100 to 1:200 is suggested. Either paraffin fixation or cryofixation can be used for sample preparation to stain intracellular IL- 1beta. For ELISA also use HRP Conjugated Anti-Rabbit IgG [HalphaL] (Goat) (611-1302) for detection. - 说明
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Target Species of Antibody: Human
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2
- 注意事项
- Minimize freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Aliquot and store at -20°C. Avoid freeze-thaw cycles.
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- 抗原
- IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))
- 别名
- IL1B (IL1B 产品)
- 别名
- IL-1 antibody, IL1-BETA antibody, IL1F2 antibody, IL-1BETA antibody, IL1beta antibody, il1-b antibody, zgc:111873 antibody, IL-1B antibody, IL-1beta antibody, Il-1b antibody, IL1B antibody, IL-1 beta antibody, IL-1b antibody, interleukin 1 beta antibody, interleukin 1, beta antibody, IL1B antibody, il1b antibody, Il1b antibody
- 背景
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Name/Gene ID: IL1B
Family: IL-1
Synonyms: IL1B, IL-1B, IL-1 beta, IL1-BETA, Preinterleukin 1 beta, Pro-interleukin-1-beta, Catabolin, IL-1, IL1F2, Interleukin 1, beta, Interleukin-1 beta - 基因ID
- 3553
- UniProt
- P01584
- 途径
- NF-kappaB Signaling, Interferon-gamma Pathway, TLR signaling, Negative Regulation of Hormone Secretion, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Autophagy, Cancer Immune Checkpoints, Inflammasome
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