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- 抗原 See all CD137 (TNFRSF9) 抗体
- CD137 (TNFRSF9) (Tumor Necrosis Factor Receptor Superfamily, Member 9 (TNFRSF9))
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适用
- 小鼠
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宿主
- 大鼠
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克隆类型
- 单克隆
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标记
- This CD137 antibody is conjugated to Biotin
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应用范围
- Flow Cytometry (FACS)
- 品牌
- BD Pharmingen™
- 产品特性
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The 1AH2 clone (subclone of 53A2) has been reported to react with CD137, a member of the TNFR/NGFR superfamily. The expression of CD137 has been reported to be detectable from day 3 and peaks around day 6 after activation with ConA, PMA plus ionomycin, or immobilized anti-CD3e (Cat. No. 553058). Monomers, dimers, or tetramers of the 4-1BB antigen are expressed, upon activation, on the surface of splenic T lymphocytes, thymocytes, intestinal intraepithelial T lymphocytes (IEL), and some T cell lines and clones. While stimulating T cells by IL-2, IL-4, or anti-CD28 alone does not result in the expression of CD137, addition of IL-2, IL-4, anti-CD28, or syngeneic accessory cells to splenic T cells stimulated via TCR/CD3 can result in a high level of CD137 expression. CD137 has also been reported to be observed on IL-2 activated NK cells, but not on freshly isolated NK cells. It has been demonstrated that 4-1BB physically associates with p56 [lck]through a Cys-Arg-Cys-Pro binding site in its cytoplasmic domain, the same motif in the cytoplasmic tail of the CD4 and CD8a molecules is responsible for association with p56 [lck]. A signaling function for the CD137 Molecule in mouse T cells is indicated by reports in which cross-linking of CD137 with 1AH2 mAb resulted in enhanced proliferation of CD3e-activated splenic T cells and IEL and in enhanced cytolytic activity of IEL in response to immobilized anti-CD3e. In addition to extracellular matrix proteins which bind to CD137, a 97- kDa dimer of the TNF/NGF superfamily has been reported to be a ligand for 4-1BB (4-1BBL). This molecule has been detected on Con A-activated T cells, LPS-activated macrophages, and anti-μ-activated splenic B cells. Interaction between T and B cells through 4-1BB/4-1BBL is reported to play a role in antigen presentation, further supporting a costimulatory role for CD137 in the immune response of T lymphocytes. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature.
BD Pharmingen™ Biotin Rat Anti-Mouse CD137 - Biotin - Clone 1AH2 - Isotype Rat IgG1, κ - Reactivity Ms - 0.1 mg - 纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- 免疫原
- Recombinant mouse 4-1BB
- 克隆位点
- 1AH2
- 亚型
- IgG1 kappa
- Top Product
- Discover our top product TNFRSF9 Primary Antibody
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
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- 浓度
- 0.5 mg/mL
- 缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
- 储存条件
- 4 °C
- 储存方法
- Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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CD27-mediated activation of murine NK cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 164, Issue 4, pp. 1741-5, (2000) (PubMed).
: "CD28-independent, TRAF2-dependent costimulation of resting T cells by 4-1BB ligand." in: The Journal of experimental medicine, Vol. 187, Issue 11, pp. 1849-62, (1998) (PubMed).
: "Costimulation of CD28- T lymphocytes by 4-1BB ligand." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 158, Issue 2, pp. 551-9, (1997) (PubMed).
: "Signals through 4-1BB are costimulatory to previously activated splenic T cells and inhibit activation-induced cell death." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 158, Issue 6, pp. 2600-9, (1997) (PubMed).
: "Role of 4-1BB ligand in costimulation of T lymphocyte growth and its upregulation on M12 B lymphomas by cAMP." in: The Journal of experimental medicine, Vol. 181, Issue 3, pp. 985-92, (1995) (PubMed).
: "Potential role of 4-1BB in T cell activation. Comparison with the costimulatory molecule CD28." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 155, Issue 7, pp. 3360-7, (1995) (PubMed).
: "Regulation of 4-1BB expression by cell-cell interactions and the cytokines, interleukin-2 and interleukin-4." in: European journal of immunology, Vol. 25, Issue 2, pp. 488-94, (1995) (PubMed).
: "Functional analysis of T-cell antigen 4-1BB in activated intestinal intra-epithelial T lymphocytes." in: Immunology letters, Vol. 41, Issue 2-3, pp. 177-84, (1995) (PubMed).
: "Novel T cell antigen 4-1BB associates with the protein tyrosine kinase p56lck1." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 151, Issue 3, pp. 1255-62, (1993) (PubMed).
: "Inducible T cell antigen 4-1BB. Analysis of expression and function." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 150, Issue 3, pp. 771-81, (1993) (PubMed).
: "T-cell activation molecule 4-1BB binds to extracellular matrix proteins." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 21, pp. 10360-4, (1992) (PubMed).
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CD27-mediated activation of murine NK cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 164, Issue 4, pp. 1741-5, (2000) (PubMed).
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- 抗原
- CD137 (TNFRSF9) (Tumor Necrosis Factor Receptor Superfamily, Member 9 (TNFRSF9))
- 别名
- CD137 (TNFRSF9 产品)
- 别名
- TNFRSF9 antibody, zgc:136557 antibody, 4-1BB antibody, CD137 antibody, CDw137 antibody, ILA antibody, A930040I11Rik antibody, AA408498 antibody, AI325004 antibody, Cd137 antibody, Ly63 antibody, tumor necrosis factor receptor superfamily member 9 antibody, TNF receptor superfamily member 9 antibody, tumor necrosis factor receptor superfamily, member 9a antibody, tumor necrosis factor receptor superfamily, member 9 antibody, TNFRSF9 antibody, tnfrsf9a antibody, Tnfrsf9 antibody
- 背景
- Synonyms: 4-1BB, Ly-63
- 途径
- Cancer Immune Checkpoints
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