anti-人 MET 抗体 for Cell-ELISA

Recommended MET Antibody (supplied by: Log in to see )

抗原
Met Proto-Oncogene (MET) 抗体
  • AUTS9
  • HGFR
  • RCCP2
  • c-Met
  • AI838057
  • HGF
  • Par4
  • Hgfr
  • c-met
  • MET proto-oncogene, receptor tyrosine kinase
  • met proto-oncogene
  • MET
  • Met
适用

710
292
222
20
15
9
6
6
5
3
3
3
3
2
2
1
1
1
1
宿主
大鼠
444
272
5
4
4
克隆类型
单克隆
标记
This MET antibody is un-conjugated
25
24
24
19
19
13
12
10
8
7
7
7
7
7
6
6
6
6
6
6
6
5
5
5
5
5
5
4
1
1
应用范围
Cell-ELISA (cELISA), Flow Cytometry (FACS)
513
211
141
129
101
85
65
39
24
19
13
10
7
6
3
2
1
1
1
1
1
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产品编号 ABIN1381745
$ 461.73
加上运输费 $45.00

General

抗原 Met Proto-Oncogene (MET) 抗体
适用
(710), (292), (222), (20), (15), (9), (6), (6), (5), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1)
宿主 大鼠
(444), (272), (5), (4), (4)
克隆类型 (克隆位点)
单克隆   ( )
标记 This MET antibody is un-conjugated
(25), (24), (24), (19), (19), (13), (12), (10), (8), (7), (7), (7), (7), (7), (6), (6), (6), (6), (6), (6), (6), (5), (5), (5), (5), (5), (5), (4), (1), (1)
应用范围 Cell-ELISA (cELISA), Flow Cytometry (FACS)
(513), (211), (141), (129), (101), (85), (65), (39), (24), (19), (13), (10), (7), (6), (3), (2), (1), (1), (1), (1), (1)
Pubmed 3个引用可参考
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MET抗体详情

MET目标详情 使用细节 贮存及处理 References for anti-MET antibody (ABIN1381745) 图像
特异性 Anti-human c-Met
产品特性 Selection: Based on recognition of the complete native protein expressed on transfected mammalian cells
纯化方法 Protein G
免疫原 genetic immunisation with cDNA encoding human c-Met
克隆位点 BCI-3E7
亚型 IgG2b, kappa

MET目标详情

MET抗体详情 使用细节 贮存及处理 References for anti-MET antibody (ABIN1381745) 图像 回页首
抗原
别名 c-Met (MET Antibody 摘要)
背景 C-MET is a receptor tyrosine kinase that is activated upon Hepatocyte Growth Factor (HGF) binding. c-MET is produced as a single-chain precursor and plays en essential role in embryonic development and wound healing. It belongs to the class of Single-pass type I membrane proteins and it is normally expressed by cells of epithelial origin while the expression of HGF is restricted to cells of mesenchymal origin in an almost perfect paracrine paradigm. Overexpression of c-MET can be found in several kinds of tumor progression.
UniProt P08581
途径 RTK signaling, Carbohydrate Homeostasis, Synaptic Membrane, Signaling of Hepatocyte Growth Factor Receptor

使用细节

MET抗体详情 MET目标详情 贮存及处理 References for anti-MET antibody (ABIN1381745) 图像 回页首
应用备注 Flow cytometry: 1.2 µg/10^6 cells
CELISA: 1:200 - 1:400
For each application a titration should be performed to determine the optimal concentration.
限制 仅限研究用

贮存及处理

MET抗体详情 MET目标详情 使用细节 References for anti-MET antibody (ABIN1381745) 图像 回页首
缓冲液 PBS, pH 7.2
注意事项 Avoid repeated freezing and thawing.
储存条件 4 °C
储存方法 short term: 2 °C - 8 °C, long term: -20 °C

References for anti-MET antibody (ABIN1381745)

MET抗体详情 MET目标详情 使用细节 贮存及处理 图像 回页首
背景

Cooper: "The met oncogene: from detection by transfection to transmembrane receptor for hepatocyte growth factor." in: Oncogene, Vol. 7, Issue 1, pp. 3-7, 1992

Galland, Stefanova, Lafage, Birnbaum: "Localization of the 5' end of the MCF2 oncogene to human chromosome 15q15----q23." in: Cytogenetics and cell genetics, Vol. 60, Issue 2, pp. 114-6, 1992

Bottaro, Rubin, Faletto, Chan, Kmiecik, Vande Woude, Aaronson: "Identification of the hepatocyte growth factor receptor as the c-met proto-oncogene product." in: Science (New York, N.Y.), Vol. 251, Issue 4995, pp. 802-4, 1991

图像

MET抗体详情 MET目标详情 使用细节 贮存及处理 References for anti-MET antibody (ABIN1381745) 回页首
厂商提供的图像
Flow Cytometry (FACS) image for anti-Met Proto-Oncogene (MET) antibody (ABIN1381745) FACS analysis of BOSC23 cells using BCI-3E7. BOSC23 cells were transiently transfecte...
SDS-PAGE (SDS) image for anti-Met Proto-Oncogene (MET) antibody (ABIN1381745) SDS-PAGE analysis of purified BCI-3E7 monoclonal antibody. Lane 1: molecular weight m...