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c-MET 抗体 (C-Term)

Name: c-MET 抗体 (C-Term)
Brand: antibodies-online
SKU: ABIN2854819
Availability: OutOfStock

MET 适用: 人 WB, IF, ICC, IP 宿主: 兔 Polyclonal unconjugated

WB Image Sample(30 ug whole cell lysate) A:HeLa S3, 5% SDS PAGE antibody diluted at 1:1000

ICC/IF Image c-Met antibody [c3], c-term detects c-Met protein at cytoplasm by immunofluorescent analysis.

IHC-P Image Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using c-Met, antibody at 1:100 dilution.

IHC

IP Image Immunoprecipitation of c-Met protein from HeLa whole cell extracts using 5 μg of c-Met antibody [C3], C-term, Western blot analysis was performed using c-Met antibody [C3], C-term, EasyBlot anti-Rabbit IgG was used as a secondary reagent.

MUC20 knockdown inhibits HGF/MET signalling in PDAC cells.

5 images

产品编号 ABIN2854819

发货至: 中国

Datasheet as PDF

抗原 See all c-MET (MET) 抗体

c-MET (MET) (Met Proto-Oncogene (MET))
抗原表位
49

35

28

21

16

11

11

8

7

7

7

6

5

5

4

4

3

3

3

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
C-Term
适用
250

144

98

8

6

4

4

4

2

2

2

1

1

1

1
人
宿主
221

39

3

2
兔
Compatible Secondaries
克隆类型
209

56
多克隆
标记
131

20

13

12

9

9

9

9

9

9

8

7

3

3

3

3

3

1

1

1

1

1
This c-MET antibody is un-conjugated
应用范围
181

105

51

43

30

27

26

24

14

8

7

7

4

4

1

1

1
Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunoprecipitation (IP)

交叉反应

Chimpanzee

交叉反应（详细）

Chimpanzee (100 %)

产品特性

Rabbit Polyclonal antibody to MET (met proto-oncogene (hepatocyte growth factor receptor))
c-Met antibody [C3], C-term

纯化方法

Purified by antigen-affinity chromatography.

免疫原

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human c-Met. The exact sequence is proprietary.

亚型

IgG

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MET 适用: 人 WB, ELISA, IHC, IF, FACS 宿主: 兔 Monoclonal 2D12 unconjugated Recombinant Antibody

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MET 适用: 人 WB, ELISA, IHC, FACS, ICC 宿主: 小鼠 Monoclonal 5E9B7 unconjugated

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MET 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

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MET 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated

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MET 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Met Proto-Oncogene (MET) (pTyr1349) antibody
MET 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Met Proto-Oncogene (MET) (pTyr1003) antibody
MET 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

应用备注

Suggested dilution Reference ICC/IF 1:100-1:1000* Immunoprecipitation 1:100-1:500* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* Immunoprecipitation1:100-1:500* Western blot1:500-1:3000*

说明

Positive Control: HeLaS3

限制

仅限研究用
状态

Liquid

浓度

1 mg/mL

缓冲液

0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

储存液

Thimerosal (Merthiolate)

注意事项

This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C

储存方法

Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
抗原

c-MET (MET) (Met Proto-Oncogene (MET))

别名

C-Met (MET 产品)

别名

AUTS9 antibody, HGFR antibody, RCCP2 antibody, c-Met antibody, AI838057 antibody, HGF antibody, Par4 antibody, Hgfr antibody, c-met antibody, MET antibody, C-MET antibody, met antibody, met-A antibody, MET proto-oncogene, receptor tyrosine kinase antibody, met proto-oncogene antibody, MET proto-oncogene, receptor tyrosine kinase L homeolog antibody, MET antibody, Met antibody, met.L antibody

背景

The proto-oncogene MET product is the hepatocyte growth factor receptor and encodes tyrosine-kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. Various mutations in the MET gene are associated with papillary renal carcinoma. Two transcript variants encoding different isoforms have been found for this gene.

Cellular Localization: Membrane, Single-pass type I membrane protein

分子量

156 kDa

基因ID

4233

途径

RTK signaling, Carbohydrate Homeostasis, Synaptic Membrane, Signaling of Hepatocyte Growth Factor Receptor