anti-人 MET 抗体 for Blocking Reagent

Recommended MET Antibody (supplied by: Log in to see )

抗原
Met Proto-Oncogene (MET) 抗体
  • AUTS9
  • HGFR
  • RCCP2
  • c-Met
  • AI838057
  • HGF
  • Par4
  • Hgfr
  • c-met
  • MET proto-oncogene, receptor tyrosine kinase
  • met proto-oncogene
  • MET
  • Met
抗原表位
AA 25-932
83
80
55
48
41
30
24
18
17
15
15
15
15
14
6
6
6
6
5
5
5
4
4
4
4
4
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
适用

710
292
222
20
15
9
6
6
5
3
3
3
3
2
2
1
1
1
1
宿主
山羊
444
272
5
4
4
克隆类型
多克隆
标记
This MET antibody is un-conjugated
25
24
24
19
19
13
12
10
8
7
7
7
7
7
6
6
6
6
6
6
6
5
5
5
5
5
5
4
1
1
应用范围
Blocking Reagent (BR), Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunohistochemistry (IHC), Western Blotting (WB)
512
211
140
129
101
85
65
39
24
19
13
10
7
5
3
2
1
1
1
1
1
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产品编号 ABIN5012912
产品在该地区无法提供。

General

抗原 Met Proto-Oncogene (MET) 抗体
抗原表位 AA 25-932
(83), (80), (55), (48), (41), (30), (24), (18), (17), (15), (15), (15), (15), (14), (6), (6), (6), (6), (5), (5), (5), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
适用
(710), (292), (222), (20), (15), (9), (6), (6), (5), (3), (3), (3), (3), (2), (2), (1), (1), (1), (1)
宿主 山羊
(444), (272), (5), (4), (4)
克隆类型
多克隆
标记 This MET antibody is un-conjugated
(25), (24), (24), (19), (19), (13), (12), (10), (8), (7), (7), (7), (7), (7), (6), (6), (6), (6), (6), (6), (6), (5), (5), (5), (5), (5), (5), (4), (1), (1)
应用范围 Blocking Reagent (BR), Cytometry by Time of Flight (CyTOF), Flow Cytometry (FACS), Immunohistochemistry (IHC), Western Blotting (WB)
(512), (211), (140), (129), (101), (85), (65), (39), (24), (19), (13), (10), (7), (5), (3), (2), (1), (1), (1), (1), (1)
Pubmed 14个引用可参考
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MET抗体详情

MET目标详情 使用细节 贮存及处理 References for anti-MET antibody (ABIN5012912) 图像
特异性 Detects human HGF R/c-MET in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 30 % cross-reactivity with recombinant mouse HGF R is observed.
纯化方法 Antigen Affinity-purified
内毒素水平 <0.10 EU per 1 μg of the antibody by the LAL method.
免疫原 Mouse myeloma cell line NS0-derived recombinant human HGF R/c-MET, Glu25-Thr932, Accession # P08581
亚型 IgG

MET目标详情

MET抗体详情 使用细节 贮存及处理 References for anti-MET antibody (ABIN5012912) 图像 回页首
抗原
别名 HGF R/c-MET (MET Antibody 摘要)
背景 Alternative Names: AUTS9, c-Met, cMET, EC 2.7.10, EC 2.7.10.1, hepatocyte growth factor receptor, HGF receptor, HGF/SF receptor, HGFR, MET, Met (c-Met), met proto-oncogene (hepatocyte growth factor receptor), met proto-oncogene tyrosine kinase, oncogene MET, Proto-oncogene c-Met, RCCP2, Scatter factor receptor, SF receptor, Tyrosine-protein kinase Met
基因ID 4233
UniProt P08581
途径 RTK signaling, Carbohydrate Homeostasis, Synaptic Membrane, Signaling of Hepatocyte Growth Factor Receptor

使用细节

MET抗体详情 MET目标详情 贮存及处理 References for anti-MET antibody (ABIN5012912) 图像 回页首
应用备注 Western Blot 0.1 μg/mL
Flow Cytometry 2.5 μg/10^6 cells
Immunohistochemistry 5-15 μg/mL
Blockade of Receptor-ligand Interaction
CyTOF-ready
限制 仅限研究用

贮存及处理

MET抗体详情 MET目标详情 使用细节 References for anti-MET antibody (ABIN5012912) 图像 回页首
溶解方式 Reconstitute at 0.2 mg/mL in sterile PBS.
缓冲液 Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
储存液 Without preservative
储存条件 4 °C,-20 °C,-80 °C
储存方法 Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt,-20°C to -70°C as supplied. 1 month,2°C to 8°C under sterile conditions after reconstitution. 6 months,-20°C to -70°C under sterile conditions after reconstitution.
有效期 12 months

References for anti-MET antibody (ABIN5012912)

MET抗体详情 MET目标详情 使用细节 贮存及处理 图像 回页首
有引用在:

Kaye, Pinto, Cecchi, Reilly, Semerjian, Rabe, Gupta, Choyke, Bottaro: "Tumor and Plasma Met Levels in Non-Metastatic Prostate Cancer." in: PLoS ONE, Vol. 11, Issue 6, pp. e0157130, 2016 Method employed by authors: ELISA (Detection) (ELISA (Detection)) (Sample species: Human).

Barrow-McGee, Kishi, Joffre, Ménard, Hervieu, Bakhouche, Noval, Mai, Guzmán, Robert-Masson, Iturrioz, Hulit, Brennan, Hart, Parker, Ivaska, Kermorgant: "Beta 1-integrin-c-Met cooperation reveals an inside-in survival signalling on autophagy-related endomembranes." in: Nature communications, Vol. 7, pp. 11942, 2016

Zoratti, Tanabe, Varela, Murray, Bergum, Colombo, Lang, Molinolo, Leduc, Marsault, Boerner, List: "Targeting matriptase in breast cancer abrogates tumour progression via impairment of stromal-epithelial growth factor signalling." in: Nature communications, Vol. 6, pp. 6776, 2015 Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Human).

Tabernero, Elez, Herranz, Rico, Prudkin, Andreu, Mateos, Carreras, Han, Gifford, Credi, Yin, Agarwal, Komarnitsky, Baselga: "A pharmacodynamic/pharmacokinetic study of ficlatuzumab in patients with advanced solid tumors and liver metastases." in: Clinical cancer research : an official journal of the American Association for Cancer Research, Vol. 20, Issue 10, pp. 2793-804, 2014 Method employed by authors: ELISA (Detection) (ELISA (Detection)) (Sample species: Human).

Cho, Park, Park, Lee, Lee, Kim, Kim, Yoon, Park, Kim, Cho, Jang, Park, Chi, Yoo, Jang, Kim, Kim, Jo, Park: "Identification of a pivotal endocytosis motif in c-Met and selective modulation of HGF-dependent aggressiveness of cancer using the 16-mer endocytic peptide." in: Oncogene, Vol. 32, Issue 8, pp. 1018-29, 2013 (Sample species: Human).

Muller, Trinidad, Timpson, Morton, Zanivan, van den Berghe, Nixon, Karim, Caswell, Noll, Coffill, Lane, Sansom, Neilsen, Norman, Vousden: "Mutant p53 enhances MET trafficking and signalling to drive cell scattering and invasion." in: Oncogene, Vol. 32, Issue 10, pp. 1252-65, 2013 Method employed by authors: Western Blotting (WB) (Sample species: Human).

Hasenauer, Malinger, Koschut, Pace, Matzke, von Au, Orian-Rousseau: "Internalization of Met requires the co-receptor CD44v6 and its link to ERM proteins." in: PLoS ONE, Vol. 8, Issue 4, pp. e62357, 2013 Method employed by authors: Immunocytochemistry (ICC) (Sample species: Human).

Szabo, Rasmussen, Moyer, Kosa, Schafer, Molinolo, Gutkind, Bugge: "c-Met-induced epithelial carcinogenesis is initiated by the serine protease matriptase." in: Oncogene, Vol. 30, Issue 17, pp. 2003-16, 2011 Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Human).

Xu, Nilsson, Saintigny, Cascone, Herynk, Du, Nikolinakos, Yang, Prudkin, Liu, Lee, Johnson, Wong, Girard, Gazdar, Minna, Kurie, Wistuba, Heymach: "Epidermal growth factor receptor regulates MET levels and invasiveness through hypoxia-inducible factor-1alpha in non-small cell lung cancer cells." in: Oncogene, Vol. 29, Issue 18, pp. 2616-27, 2010 Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Human).

Abella, Parachoniak, Sangwan, Park: "Dorsal ruffle microdomains potentiate Met receptor tyrosine kinase signaling and down-regulation." in: The Journal of biological chemistry, Vol. 285, Issue 32, pp. 24956-67, 2010 Method employed by authors: Immunocytochemistry (ICC) (Sample species: Human).

Schmelzer, Mutig, Schrade, Bachmann, Gerlach, Zeilinger et al.: "Effect of human patient plasma ex vivo treatment on gene expression and progenitor cell activation of primary human liver cells in multi-compartment 3D perfusion bioreactors for extra-corporeal liver ..." in: Biotechnology and bioengineering, Vol. 103, Issue 4, pp. 817-27, 2009 Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Human).

Goldoni, Humphries, Nyström, Sattar, Owens, McQuillan, Ireton, Iozzo: "Decorin is a novel antagonistic ligand of the Met receptor." in: The Journal of cell biology, Vol. 185, Issue 4, pp. 743-54, 2009 Method employed by authors: Immunocytochemistry (ICC) (Sample species: Human).

Skibinski, Elborn, Ennis: "Bronchial epithelial cell growth regulation in fibroblast cocultures: the role of hepatocyte growth factor." in: American journal of physiology. Lung cellular and molecular physiology, Vol. 293, Issue 1, pp. L69-76, 2007 Method employed by authors: Neutralization (Neut) (Sample species: Human).

Bogatkevich, Ludwicka-Bradley, Highland, Hant, Nietert, Singleton, Feghali-Bostwick, Silver: "Impairment of the antifibrotic effect of hepatocyte growth factor in lung fibroblasts from African Americans: possible role in systemic sclerosis." in: Arthritis and rheumatism, Vol. 56, Issue 7, pp. 2432-42, 2007 Method employed by authors: Neutralization (Neut) (Sample species: Human).

图像

MET抗体详情 MET目标详情 使用细节 贮存及处理 References for anti-MET antibody (ABIN5012912) 回页首
厂商提供的图像
Immunohistochemistry (IHC) image for anti-Met Proto-Oncogene (MET) (AA 25-932) antibody (ABIN5012912) HGF R/c-MET in Human Liver. HGF R/c-MET was detected in immersion fixed paraffin-embe...
Immunohistochemistry (IHC) image for anti-Met Proto-Oncogene (MET) (AA 25-932) antibody (ABIN5012912) HGF R/c-MET in Human Skin. HGF R/c-MET was detected in immersion fixed paraffin-embed...
Immunocytochemistry (ICC) image for anti-Met Proto-Oncogene (MET) (AA 25-932) antibody (ABIN5012912) HGF R/c-MET in HT-29 and U937 Human Cell Line. HGF R/c-MET was detected in immersion ...