anti-小鼠 PRAME 抗体 for Western Blotting

Recommended PRAME Antibody (supplied by: Log in to see )

抗原
Preferentially Expressed Antigen in Melanoma (PRAME) 抗体
  • PRAME
  • CT130
  • MAPE
  • OIP-4
  • OIP4
  • 4930534P07Rik
  • preferentially expressed antigen in melanoma
  • PRAME
  • Prame
抗原表位
N-Term
15
13
10
7
5
4
3
2
2
2
2
1
1
1
适用
牛, 马, 人, 小鼠, 猪, 大鼠
74
4
4
3
3
2
2
2
1
宿主

71
4
克隆类型
多克隆
标记
This PRAME antibody is un-conjugated
5
5
3
1
1
1
1
1
1
1
1
1
1
1
应用范围
Western Blotting (WB)
53
13
12
11
10
7
5
3
3
2
2
2
1
选项
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产品编号 ABIN2786465
$ 289.00
加上运输费 $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.1703398 ABIN2786466 WB Rabbit N-Term Log in to see Polyclonal 2
3.1703398 ABIN315885 ICC IF WB Rabbit N-Term, AA 1-100 Log in to see Polyclonal 0
3.1703398 ABIN5963712 WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN6290587 WB Rabbit IgG Log in to see Polyclonal 0

General

抗原 Preferentially Expressed Antigen in Melanoma (PRAME) 抗体
抗原表位 N-Term
(15), (13), (10), (7), (5), (4), (3), (2), (2), (2), (2), (1), (1), (1)
适用 牛, 马, 人, 小鼠, 猪, 大鼠
(74), (4), (4), (3), (3), (2), (2), (2), (1)
宿主
(71), (4)
克隆类型
多克隆
标记 This PRAME antibody is un-conjugated
(5), (5), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
应用范围 Western Blotting (WB)
(53), (13), (12), (11), (10), (7), (5), (3), (3), (2), (2), (2), (1)
Pubmed 2个引用可参考
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PRAME抗体详情

PRAME目标详情 使用细节 贮存及处理 References for anti-PRAME antibody (ABIN2786465) 图像
Blocking Peptide Blocking peptide for this product available: ABIN982156
序列 MERRRLWGSI QSRYISMSVW TSPRRLVELA GQSLLKDEAL AIAALELLPR
预测反应 Cow: 86%, Horse: 93%, Human: 100%, Mouse: 79%, Pig: 86%, Rat: 85%
产品特性 This is a rabbit polyclonal antibody against PRAME. It was validated on Western Blot using a cell lysate as a positive control.
纯化方法 Affinity Purified
免疫原 The immunogen is a synthetic peptide directed towards the N terminal region of human PRAME
Plasmids, Primers & others

PRAME目标详情

PRAME抗体详情 使用细节 贮存及处理 References for anti-PRAME antibody (ABIN2786465) 图像 回页首
抗原
别名 PRAME (PRAME Antibody 摘要)
背景 PRAME functions as a transcriptional repressor, inhibiting the signaling of retinoic acid through the retinoic acid receptors RARA, RARB and RARG. PRAME prevents retinoic acid-induced cell proliferation arrest, differentiation and apoptosis.This gene encodes an antigen that is predominantly expressed in human melanomas and that is recognized by cytolytic T lymphocytes. It is not expressed in normal tissues, except testis. This expression pattern is similar to that of other CT antigens, such as MAGE, BAGE and GAGE. However, unlike these other CT antigens, this gene is also expressed in acute leukemias. Five alternatively spliced transcript variants encoding the same protein have been observed for this gene.
Alias Symbols: MAPE, OIP4, CT130
Protein Interaction Partner: PRAME, RBX1, CUL2, TCEB2, TCEB1, ZNF408, FBXL12, XRN2, HIST4H4, HIST2H2BE, HIST2H2AC, HIST3H3, UBC, RARA, TP53RK, OSGEP, TPRKB, KDM1A, BAG2, LAGE3, UBL4A, SNRPB, RPS13, RPL22, RPL10, RFC4, PSMC4, HNRNPM, HNRNPH1, HNRNPF, HNRNPC, NEDD8, STK19,
Protein Size: 509
分子量 56 kDa
基因ID 23532
NCBI登录号 NM_006115, NP_006106
UniProt P78395
途径 Retinoic Acid Receptor Signaling Pathway, Nuclear Hormone Receptor Binding

使用细节

PRAME抗体详情 PRAME目标详情 贮存及处理 References for anti-PRAME antibody (ABIN2786465) 图像 回页首
应用备注 Optimal working dilutions should be determined experimentally by the investigator.
说明

Antigen size: 509 AA

限制 仅限研究用

贮存及处理

PRAME抗体详情 PRAME目标详情 使用细节 References for anti-PRAME antibody (ABIN2786465) 图像 回页首
状态 Liquid
浓度 Lot specific
缓冲液 Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
储存液 Sodium azide
注意事项 This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项 Avoid repeated freeze-thaw cycles.
储存条件 -20 °C
储存方法 For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-PRAME antibody (ABIN2786465)

PRAME抗体详情 PRAME目标详情 使用细节 贮存及处理 图像 回页首
有引用在:

Liu, Zhao, Lu, Ning, Ma, Diaz, OConnor: "A novel testis-specific protein, PRAMEY, is involved in spermatogenesis in cattle." in: Reproduction (Cambridge, England), Vol. 153, Issue 6, pp. 847-863, 2017

背景

Roman-Gomez, Jimenez-Velasco, Agirre, Castillejo, Navarro, Jose-Eneriz, Garate, Cordeu, Cervantes, Prosper, Heiniger, Torres: "Epigenetic regulation of PRAME gene in chronic myeloid leukemia." in: Leukemia research, Vol. 31, Issue 11, pp. 1521-8, 2007

图像

PRAME抗体详情 PRAME目标详情 使用细节 贮存及处理 References for anti-PRAME antibody (ABIN2786465) 回页首
厂商提供的图像
Western Blotting (WB) image for anti-Preferentially Expressed Antigen in Melanoma (PRAME) (N-Term) antibody (ABIN2786465) WB Suggested Anti-PRAME Antibody Titration: 0.2-1 ug/ml
ELISA Titer: