PRAME 抗体 (Preferentially Expressed Antigen in Melanoma) (N-Term)

Details for Product anti-PRAME Antibody No. ABIN2786465, 供应商: Log in to see
抗原
  • PRAME
  • CT130
  • MAPE
  • OIP-4
  • OIP4
  • 4930534P07Rik
  • preferentially expressed antigen in melanoma
  • PRAME
  • Prame
抗原表位
N-Term
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适用
牛, 马, 人, 小鼠, 猪, 大鼠
74
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宿主

71
4
克隆类型
多克隆
标记
This PRAME antibody is un-conjugated
5
5
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应用范围
Western Blotting (WB)
53
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选项
Supplier
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Supplier Product No.
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免疫原 The immunogen is a synthetic peptide directed towards the N terminal region of human PRAME
Blocking Peptide Blocking peptide for this product available: ABIN982156
序列 MERRRLWGSI QSRYISMSVW TSPRRLVELA GQSLLKDEAL AIAALELLPR
预测反应 Cow: 86%, Horse: 93%, Human: 100%, Mouse: 79%, Pig: 86%, Rat: 85%
产品特性 This is a rabbit polyclonal antibody against PRAME. It was validated on Western Blot using a cell lysate as a positive control.
纯化方法 Affinity Purified
Plasmids, Primers & others Plasmids, Primers & others PRAME products on genomics-online (e.g. as negative or positive controls)
抗原
别名 PRAME (PRAME Antibody 摘要)
背景 PRAME functions as a transcriptional repressor, inhibiting the signaling of retinoic acid through the retinoic acid receptors RARA, RARB and RARG. PRAME prevents retinoic acid-induced cell proliferation arrest, differentiation and apoptosis.This gene encodes an antigen that is predominantly expressed in human melanomas and that is recognized by cytolytic T lymphocytes. It is not expressed in normal tissues, except testis. This expression pattern is similar to that of other CT antigens, such as MAGE, BAGE and GAGE. However, unlike these other CT antigens, this gene is also expressed in acute leukemias. Five alternatively spliced transcript variants encoding the same protein have been observed for this gene.
Alias Symbols: MAPE, OIP4, CT130
Protein Interaction Partner: PRAME, RBX1, CUL2, TCEB2, TCEB1, ZNF408, FBXL12, XRN2, HIST4H4, HIST2H2BE, HIST2H2AC, HIST3H3, UBC, RARA, TP53RK, OSGEP, TPRKB, KDM1A, BAG2, LAGE3, UBL4A, SNRPB, RPS13, RPL22, RPL10, RFC4, PSMC4, HNRNPM, HNRNPH1, HNRNPF, HNRNPC, NEDD8, STK19,
Protein Size: 509
分子量 56 kDa
基因ID 23532
NCBI登录号 NM_006115, NP_006106
UniProt P78395
途径 Retinoic Acid Receptor Signaling Pathway, Nuclear Hormone Receptor Binding
应用备注 Optimal working dilutions should be determined experimentally by the investigator.
说明

Antigen size: 509 AA

限制 仅限研究用
状态 Liquid
浓度 Lot specific
缓冲液 Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
储存液 Sodium azide
注意事项 This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项 Avoid repeated freeze-thaw cycles.
储存条件 -20 °C
储存方法 For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
厂商提供的图像
Western Blotting (WB) image for anti-Preferentially Expressed Antigen in Melanoma (PRAME) (N-Term) antibody (ABIN2786465) WB Suggested Anti-PRAME Antibody Titration: 0.2-1 ug/ml
ELISA Titer:
有引用在: Liu, Zhao, Lu, Ning, Ma, Diaz, OConnor: "A novel testis-specific protein, PRAMEY, is involved in spermatogenesis in cattle." in: Reproduction (Cambridge, England), Vol. 153, Issue 6, pp. 847-863, 2017 (PubMed).

背景 Roman-Gomez, Jimenez-Velasco, Agirre, Castillejo, Navarro, Jose-Eneriz, Garate, Cordeu, Cervantes, Prosper, Heiniger, Torres: "Epigenetic regulation of PRAME gene in chronic myeloid leukemia." in: Leukemia research, Vol. 31, Issue 11, pp. 1521-8, 2007 (PubMed).

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