anti-人 PRAME 抗体 for Flow Cytometry

Recommended PRAME Antibody (supplied by: Log in to see )

抗原
Preferentially Expressed Antigen in Melanoma (PRAME) 抗体
  • PRAME
  • CT130
  • MAPE
  • OIP-4
  • OIP4
  • 4930534P07Rik
  • preferentially expressed antigen in melanoma
  • PRAME
  • Prame
抗原表位
AA 476-502, C-Term
15
13
10
6
6
3
3
2
2
2
2
1
1
1
适用

74
5
5
4
4
3
2
2
1
宿主

71
4
克隆类型
多克隆
标记
This PRAME antibody is un-conjugated
5
5
3
1
1
1
1
1
1
1
1
1
1
1
应用范围
Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
53
13
12
11
9
7
4
3
3
2
2
2
1
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产品编号 ABIN390677
$ 335.50
加上运输费 $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
14.453772 ABIN3002772 FACS IHC WB Rabbit IgG Log in to see Polyclonal 0
1 ABIN5531568 FACS IHC (p) WB Rabbit Ig Fraction AA 476-502, C-Term Log in to see Polyclonal 0
1 ABIN1811266 FACS IHC IHC (p) WB Rabbit AA 476-502 Log in to see Polyclonal 0
1 ABIN2795004 FACS IHC WB Rabbit Ig Fraction AA 476-502, C-Term Log in to see Polyclonal 0

General

抗原 Preferentially Expressed Antigen in Melanoma (PRAME) 抗体
抗原表位 AA 476-502, C-Term
(15), (13), (10), (6), (6), (3), (3), (2), (2), (2), (2), (1), (1), (1)
适用
(74), (5), (5), (4), (4), (3), (2), (2), (1)
宿主
(71), (4)
克隆类型 (克隆位点)
多克隆   ( )
标记 This PRAME antibody is un-conjugated
(5), (5), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
应用范围 Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(53), (13), (12), (11), (9), (7), (4), (3), (3), (2), (2), (2), (1)
Pubmed 找到1个引用
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PRAME抗体详情

PRAME目标详情 使用细节 贮存及处理 References for anti-PRAME antibody (ABIN390677) 图像
特异性 This MAPE antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 480~509 amino acids from the C-terminal region of human MAPE.
纯化方法 This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
免疫原 This MAPE antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 476-502 AA from the C-terminal region of human MAPE.
克隆位点 RB18763
亚型 Ig Fraction
Plasmids, Primers & others

PRAME目标详情

PRAME抗体详情 使用细节 贮存及处理 References for anti-PRAME antibody (ABIN390677) 图像 回页首
抗原
别名 MAPE (PRAME Antibody 摘要)
背景 MAPE is an antigen that is predominantly expressed in human melanomas and that is recognized by cytolytic T lymphocytes. It is not expressed in normal tissues, except testis. This expression pattern is similar to that of other CT antigens, such as MAGE, BAGE and GAGE. However, unlike these other CT antigens, its gene is also expressed in acute leukemias.
Synonyms: Melanoma antigen preferentially expressed in tumors, Preferentially expressed antigen of melanoma, OPA-interacting protein 4, OIP4, MAPE, PRAME
分子量 57890 DA
基因ID 23532
UniProt P78395
途径 Retinoic Acid Receptor Signaling Pathway, Nuclear Hormone Receptor Binding

使用细节

PRAME抗体详情 PRAME目标详情 贮存及处理 References for anti-PRAME antibody (ABIN390677) 图像 回页首
应用备注 WB = 1:1000, IHC (p) = 1:50-100, FACS = 1:10-50
限制 仅限研究用

贮存及处理

PRAME抗体详情 PRAME目标详情 使用细节 References for anti-PRAME antibody (ABIN390677) 图像 回页首
状态 Liquid
浓度 2 mg/mL
缓冲液 PBS with 0.09 % (W/V) sodium azide
储存液 Sodium azide
注意事项 This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件 4 °C/-20 °C
储存方法 Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
有效期 6 months

References for anti-PRAME antibody (ABIN390677)

PRAME抗体详情 PRAME目标详情 使用细节 贮存及处理 图像 回页首
背景

Rezvani, Yong, Tawab, Jafarpour, Eniafe, Mielke, Savani, Keyvanfar, Li, Kurlander, Barrett: "Ex vivo characterization of polyclonal memory CD8+ T-cell responses to PRAME-specific peptides in patients with acute lymphoblastic leukemia and acute and chronic myeloid leukemia." in: Blood, Vol. 113, Issue 10, pp. 2245-55, 2009

图像

PRAME抗体详情 PRAME目标详情 使用细节 贮存及处理 References for anti-PRAME antibody (ABIN390677) 回页首
厂商提供的图像
Western Blotting (WB) image for anti-Preferentially Expressed Antigen in Melanoma (PRAME) (AA 476-502), (C-Term) antibody (ABIN390677) Western blot analysis of MAPE antibody (C-term) (ABIN390677) in K562 cell line lysate...
Immunohistochemistry (IHC) image for anti-Preferentially Expressed Antigen in Melanoma (PRAME) (AA 476-502), (C-Term) antibody (ABIN390677) MAPE Antibody (C-term) (ABIN390677) IHC analysis in formalin fixed and paraffin embed...
Flow Cytometry (FACS) image for anti-Preferentially Expressed Antigen in Melanoma (PRAME) (AA 476-502), (C-Term) antibody (ABIN390677) MAPE Antibody (C-term) (ABIN390677) flow cytometry analysis of K562 cells (bottom his...
Flow Cytometry (FACS) image for anti-Preferentially Expressed Antigen in Melanoma (PRAME) (AA 476-502), (C-Term) antibody (ABIN390677) Overlay histogram showing Hela cells stained with ABIN2840972 (green line). The cells...
Western Blotting (WB) image for anti-Preferentially Expressed Antigen in Melanoma (PRAME) (AA 476-502), (C-Term) antibody (ABIN390677) All lanes : Anti-MAPE Antibody (C-term) at 1:2000 dilution Lane 1: Hela whole cell ly...