This Prolactin Receptor protein is labelled with His tag.
原理
Cynomolgus PRLR Protein
序列
Gln25-Thr236
产品特性
Recombinant Cynomolgus PRLR Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln25-Thr236.
纯度
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
过滤
0.22 μm filtered
内毒素水平
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Cynomolgus PRLR, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Anti-PRLR Antibody, hFc Tag with the EC50 of 43.9ng/ml determined by ELISA. See testing image for detail.
PRLR
宿主: 小鼠
宿主: HEK-293 Cells
Recombinant
> 90 % as determined by SDS-PAGE
限制
仅限研究用
状态
Lyophilized
溶解方式
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
缓冲液
Lyophilized from 0.22μm filtered solution in 50 mM Tris, 150 mM NaCl ( pH 7.5). Normally 8 % trehalose is added as protectant before lyophilization.
储存条件
-20 °C,-80 °C
储存方法
-20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Prolactin receptor (PRLR) is highly expressed in a subset of human breast cancer and prostate cancer, which makes it a potential target for cancer treatment. In clinical trials, the blockade of PRLR was shown to be safe but with poor efficacy. It is therefore urgent to develop new therapies against PRLR target. Bispecific antibodies (BsAbs) could guide immune cells toward tumor cells, and produced remarkable effects in some cancers.
分子量
25.75 kDa. Due to glycosylation, the protein migrates to 35-50 kDa based on Tris-Bis PAGE result.