Recombinant Human IL-3 R alpha/CD123 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Thr19-Arg305.
纯度
> 95 % as determined by Tris-Bis PAGE
过滤
0.22 μm filtered
内毒素水平
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human IL-3 R alpha, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-IL-3 R alpha Antibody, hFc Tag with the EC50 of 13.2ng/ml determined by ELISA. The affinity constant of 0.276 μM as determined in SPR assay (Biacore T200). See testing image for detail.
IL3RA
宿主: 人
宿主: HEK-293 Cells
Recombinant
>95 % as determined by SDS-PAGE.
Active
限制
仅限研究用
状态
Lyophilized
溶解方式
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
缓冲液
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
储存条件
-20 °C,-80 °C
储存方法
-20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Interleukin-3 receptor subunit alpha, also known as IL-3 receptor subunit alpha, IL-3R-alpha, CD123, and IL3RA, is a single-pass type I membrane protein which belongs to the type I cytokine receptor family and Type 5 subfamily.The specific alpha subunit of the interleukin-3 receptor (IL-3Ralpha, CD123) is strongly expressed in various leukemic blasts and leukemic stem cells and seems to be an excellent target for the therapy of leukemias.
分子量
33.9 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.