This SARS-CoV-2 Spike S1 protein is labelled with His-Avi Tag.
原理
SARS-COV-2 Spike S1 (D614G) Protein
序列
Gln14-Arg683 (D614G)
产品特性
Recombinant SARS-COV-2 Spike S1 (D614G) Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln14-Arg683(D614G).
纯度
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
过滤
0.22 μm filtered
内毒素水平
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized SARS-CoV-2 S1 (D614G) , His Tag at 2μg/ml (100μl/Well) on plate.Dose response curve for Human ACE2, hFc Tag with the EC50 of 42ng/ml determined by ELISA. See testing image for detail.
宿主: SARS Coronavirus-2 (SARS-CoV-2)
宿主: HEK-293 Cells
Recombinant
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
限制
仅限研究用
状态
Lyophilized
溶解方式
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
缓冲液
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
储存条件
-20 °C,-80 °C
储存方法
-20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
E2 Protein, Surface Glycoprotein Protein, S Protein
物质类
Viral Protein
背景
The SARS-CoV-2 spike (S) protein is the target of vaccine design efforts to end the COVID-19 pandemic. Despite a low mutation rate, isolates with the D614G substitution in the S protein appeared early during the pandemic, and are now the dominant form worldwide. Here, we analyze the D614G mutation in the context of a soluble S ectodomain construct.
分子量
77.9 kDa. Due to glycosylation, the protein migrates to 110-120 kDa based on Tris-Bis PAGE result.