SARS-CoV-2 Spike S1 Protein (AA 16-685, N501Y) (His tag)
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Quick Overview for SARS-CoV-2 Spike S1 Protein (AA 16-685, N501Y) (His tag) (ABIN7274394)
抗原
See all SARS-CoV-2 Spike S1 蛋白蛋白类型
生物活性
宿主
资源
纯度
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产品特性
- AA 16-685, N501Y
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标记
- This SARS-CoV-2 Spike S1 protein is labelled with His tag.
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原理
- SARS-COV-2 Spike S1 (N501Y) Protein
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序列
- Val16-Arg685
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产品特性
- Recombinant SARS-COV-2 Spike S1 (N501Y) Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Val16-Arg685.
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过滤
- 0.22 μm filtered
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内毒素水平
- Less than 1 EU per μg by the LAL method.
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Biological Activity Comment
- Immobilized SARS-COV-2 Spike S1 (N501Y) , His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 66.2ng/ml determined by ELISA.,SARS-COV-2 Spike S1 (N501Y) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of SARS-COV-2 Spike S1 (N501Y) is greater than 90% as determined by SEC-HPLC.
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SARS-CoV-2 Spike S1 protein (His tag)
宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. SDS, Func
SARS-CoV-2 Spike S1 (AA 14-683), (B.1.1.529 - Omicron) (Active) protein (His tag)宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
SARS-CoV-2 Spike S1 (RBD) protein (His tag,AVI tag,Biotin)宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: HEK-293 Cells Recombinant > 90 % as determined by SDS-PAGE. > 90 % as determined by SEC-MALS.
SARS-CoV-2 Spike S1 (AA 14-667) (Active) protein (hFc-Avi Tag)宿主: SARS Coronavirus (SARS-CoV) 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
SARS-CoV-2 Spike S1 (AA 14-683), (C.37 - Lambda) (Active) protein (His tag)宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
SARS-CoV-2 Spike S1 (AA 14-683), (D614G) (Active) protein (His-Avi Tag)宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
SARS-CoV-2 Spike S1 (AA 14-683), (BA.4 - Omicron), (BA.5 - Omicron), (BA.5.2 - Omicron) (Active) protein (His tag)宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
SARS-CoV-2 Spike S1 (AA 16-685), (E484K), (K417N), (N501Y) (Active) protein (His tag)宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
SARS-CoV-2 Spike S1 (AA 14-683), (B.1.617.2 - delta) (Active) protein (His tag)宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
SARS-CoV-2 Spike S1 (AA 14-683), (P.1 - gamma), (P.1.1 - Gamma), (P.1.2 - Gamma) (Active) protein (His tag)宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.
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缓冲液
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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储存条件
- -20 °C,-80 °C
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储存方法
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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有效期
- 12 months
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- SARS-CoV-2 Spike S1
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物质类
- Viral Protein
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背景
- A new variant of SARS-CoV-2 is spreading in the UK and is rapidly becoming a global threat.It is characterised by multiple mutations in the spike protein. Among them, N501Y is of major concern because it involves one of the six key amino acid residues determining a tight interaction of the SARS-CoV-2 receptor-binding domain (RBD) with its cellular receptor angiotensin-converting enzyme 2 (ACE2).
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分子量
- 76 kDa. Due to glycosylation, the protein migrates to 115-140 kDa based on Bis-Tris PAGE result.
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NCBI登录号
- YP_009724390
抗原
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