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SARS-CoV-2 Spike S1 Protein (RBD) (His tag,AVI tag,Biotin)

This Recombinant SARS-CoV-2 Spike S1 protein is expressed in HEK-293 Cells and has been mentioned in 5+ publications.

Biotinylated 2019-nCoV (COVID-19) S protein RBD, His,Avitag (MALS verified) on SDS-PAGE under reducing (R) condition. (SARS-CoV-2 Spike S1 Protein (RBD) (His tag,AVI tag,Biotin))

SDS

Immobilized Biotinylated SARS-CoV-2 S protein RBD, His,Avitag (ABIN6952456,ABIN6952463) at 1 μg/mL (100 μL/well) on streptavidin precoated (0. (SARS-CoV-2 Spike S1 Protein (RBD) (His tag,AVI tag,Biotin))

ELISA

The purity of Biotinylated 2019-nCoV (COVID-19) S protein RBD, His,Avitag (MALS verified) ( ABIN6952456) was more than 90% in HP-SEC, and around 45-55 kDa verified by SEC-MALS. (SARS-CoV-2 Spike S1 Protein (RBD) (His tag,AVI tag,Biotin))

Immobilized Human ACE2, Fc Tag (MALS verified) ( ABIN6952459) at 2 μg/mL (100 μL/well) can bind Biotinylated 2019-nCoV (COVID-19) S protein RBD, His,Avitag (MALS verified) ( ABIN6952456) with a linear range of 4-125 ng/mL (QC tested). (SARS-CoV-2 Spike S1 Protein (RBD) (His tag,AVI tag,Biotin))

ELISA

4 images

(5 references)

产品编号 ABIN6952456

发货至: 中国

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Quick Overview for SARS-CoV-2 Spike S1 Protein (RBD) (His tag,AVI tag,Biotin) (ABIN6952456)

抗原

See all SARS-CoV-2 Spike S1 蛋白

SARS-CoV-2 Spike S1

蛋白类型

Recombinant

宿主

SARS Coronavirus-2 (SARS-CoV-2)

资源

HEK-293 Cells

纯度

> 90 % as determined by SDS-PAGE. > 90 % as determined by SEC-MALS.

Discover our top product SARS-CoV-2 Spike S1 蛋白

产品特性

RBD

标记

This SARS-CoV-2 Spike S1 protein is labelled with His tag,AVI tag,Biotin.

序列

AA 319-541

特异性

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

产品特性

Biotinylated 2019-nCoV (COVID-19) S protein RBD, His,Avitag (MALS verified) is expressed from human 293 cells (HEK293). It contains AA Arg 319 - Phe 541 (Accession # QHD43416.1). Predicted N-terminus: Arg 319 This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag.

内毒素水平

Less than 1.0 EU per μg by the LAL method.
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SARS-CoV-2 Spike S1 protein (His tag)
宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. SDS, Func

SARS-CoV-2 Spike S1 (AA 14-683), (B.1.1.529 - Omicron) (Active) protein (His tag)
宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active

SARS-CoV-2 Spike S1 (AA 14-667) (Active) protein (hFc-Avi Tag)
宿主: SARS Coronavirus (SARS-CoV) 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active

SARS-CoV-2 Spike S1 (AA 16-685), (N501Y) (Active) protein (His tag)
宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 90 % as determined by HPLC Active

SARS-CoV-2 Spike S1 (AA 14-683), (C.37 - Lambda) (Active) protein (His tag)
宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active

SARS-CoV-2 Spike S1 (AA 14-683), (D614G) (Active) protein (His-Avi Tag)
宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active

SARS-CoV-2 Spike S1 (AA 14-683), (BA.4 - Omicron), (BA.5 - Omicron), (BA.5.2 - Omicron) (Active) protein (His tag)
宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active

SARS-CoV-2 Spike S1 (AA 16-685), (E484K), (K417N), (N501Y) (Active) protein (His tag)
宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active

SARS-CoV-2 Spike S1 (AA 14-683), (B.1.617.2 - delta) (Active) protein (His tag)
宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active

SARS-CoV-2 Spike S1 (AA 14-683), (P.1 - gamma), (P.1.1 - Gamma), (P.1.2 - Gamma) (Active) protein (His tag)
宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active

应用备注

The protein has a calculated MW of 28.7 kDa. The protein migrates as 34-37 kDa under reducing (R) condition due to glycosylation.

说明

Ready-to-use AvitagTM biotinylated protein:
The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.

This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled.

限制

仅限研究用
状态

Lyophilized

缓冲液

PBS, pH 7.4

注意事项

Please avoid repeated freeze-thaw cycles.

储存条件

-20 °C,-80 °C

储存方法

For long term storage, the product should be stored at lyophilized state at -20°C or lower. This product is stable aftr storage at: -20°C to -70°C for 12 months in lyophilized state, -70°C for 3 months under sterile conditions after reconstitution.
Dogan, Kozhaya, Placek, Gunter, Yigit, Hardy, Plassmeyer, Coatney, Lillard, Bukhari, Kleinberg, Hayes, Arditi, Klapper, Merin, Liang, Gupta, Alpan, Unutmaz: "SARS-CoV-2 specific antibody and neutralization assays reveal the wide range of the humoral immune response to virus." in: Communications biology, Vol. 4, Issue 1, pp. 129, (2021) (PubMed).

Bell, Powell, Rodriguez, Cochran, Kim: "Neutralizing antibodies targeting the SARS-CoV-2 receptor binding domain isolated from a naïve human antibody library." in: Protein science : a publication of the Protein Society, Vol. 30, Issue 4, pp. 716-727, (2021) (PubMed).

Prieto-Fernández, Egia-Mendikute, Vila-Vecilla, Bosch, Barreira-Manrique, Lee, García-Del Río, Antoñana-Vildosola, Jiménez-Lasheras, Moreno-Cugnon, Jiménez-Barbero, Berra, Ereño-Orbea, Palazon: "Hypoxia reduces cell attachment of SARS-CoV-2 spike protein by modulating the expression of ACE2, neuropilin-1, syndecan-1 and cellular heparan sulfate." in: Emerging microbes & infections, Vol. 10, Issue 1, pp. 1065-1076, (2021) (PubMed).

Mulligan, Lyke, Kitchin, Absalon, Gurtman, Lockhart, Neuzil, Raabe, Bailey, Swanson, Li, Koury, Kalina, Cooper, Fontes-Garfias, Shi, Türeci, Tompkins, Walsh, Frenck, Falsey, Dormitzer, Gruber, Şahin et al.: "Phase I/II study of COVID-19 RNA vaccine BNT162b1 in adults. ..." in: Nature, Vol. 586, Issue 7830, pp. 589-593, (2020) (PubMed).

Guo, Yao, Yang, Zhang, Qi, Gou, Xi, Liu, Zhang, Cheng, Wang, Rong, Chen, Kong: "Plasma-activated water: An alternative disinfectant for S protein inactivation to prevent SARS-CoV-2 infection." in: Chemical engineering journal (Lausanne, Switzerland : 1996), pp. 127742, (2020) (PubMed).
抗原

SARS-CoV-2 Spike S1

物质类

Viral Protein

背景

It's been reported that SARS-CoV-2 can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

分子量

28.2 kDa

基因ID

43740568

UniProt

P0DTC2