This SARS-CoV-2 Spike S1 protein is labelled with His tag.
原理
SARS-COV-2 Spike S1 (N501Y,K417N,E484K) Protein
序列
Val16-Arg685 (N501Y,K417N,E484K)
产品特性
Recombinant SARS-COV-2 Spike S1 (N501Y,K417N,E484K) Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Val16-Arg685(N501Y,K417N,E484K).
纯度
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
过滤
0.22 μm filtered
内毒素水平
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized SARS-COV-2 Spike S1 (N501Y,K417N,E484K) , His Tag at 0.5μg/ml (100ul/Well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 0.02μg/ml determined by ELISA. See testing image for detail.
宿主: SARS Coronavirus-2 (SARS-CoV-2)
宿主: HEK-293 Cells
Recombinant
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
限制
仅限研究用
状态
Liquid
缓冲液
Supplied as 0.22μm filtered solution in PBS ( pH 7.4).
储存条件
-80 °C
储存方法
Valid for 12 months from date of receipt when stored at -80°C., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
E2 Protein, Surface Glycoprotein Protein, S Protein
物质类
Viral Protein
背景
The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
分子量
75.9 kDa. Due to glycosylation, the protein migrates to 90-130 kDa based on Tris-Bis PAGE result.