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SARS-CoV-2 Spike S1 Protein (AA 16-685, E484K, K417N, N501Y) (His tag)

This Recombinant SARS-CoV-2 Spike S1 protein is expressed in HEK-293 Cells.
产品编号 ABIN7274391
发货至: 中国
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Quick Overview for SARS-CoV-2 Spike S1 Protein (AA 16-685, E484K, K417N, N501Y) (His tag) (ABIN7274391)

抗原

See all SARS-CoV-2 Spike S1 蛋白
SARS-CoV-2 Spike S1

蛋白类型

Recombinant

生物活性

Active

宿主

  • 90
  • 6
  • 4
  • 4
  • 2
  • 1
  • 1
SARS Coronavirus-2 (SARS-CoV-2)

资源

  • 80
  • 6
  • 3
  • 3
  • 2
  • 1
  • 1
  • 1
HEK-293 Cells

纯度

> 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC
  • 产品特性

    AA 16-685, E484K, K417N, N501Y

    标记

    This SARS-CoV-2 Spike S1 protein is labelled with His tag.

    原理

    SARS-COV-2 Spike S1 (N501Y,K417N,E484K) Protein

    序列

    Val16-Arg685(N501Y,K417N,E484K)

    产品特性

    Recombinant SARS-COV-2 Spike S1 (N501Y,K417N,E484K) Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Val16-Arg685(N501Y,K417N,E484K).

    过滤

    0.22 μm filtered

    内毒素水平

    Less than 1 EU per μg by the LAL method.

    Biological Activity Comment

    Immobilized SARS-COV-2 Spike S1 (N501Y,K417N,E484K), His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 53.6ng/ml determined by ELISA.,SARS-COV-2 Spike S1 (N501Y,K417N,E484K) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of SARS-COV-2 Spike S1 (N501Y,K417N,E484K) is greater than 95% as determined by SEC-HPLC.
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  • 限制

    仅限研究用
  • 状态

    Liquid

    缓冲液

    Supplied as 0.22μm filtered solution in PBS ( pH 7.4).

    储存条件

    -80 °C

    储存方法

    Valid for 12 months from date of receipt when stored at -80°C., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

    有效期

    12 months
  • 抗原

    SARS-CoV-2 Spike S1

    物质类

    Viral Protein

    背景

    The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

    分子量

    75.9 kDa. Due to glycosylation, the protein migrates to 90-130 kDa based on Bis-Tris PAGE result.

    NCBI登录号

    YP_009724390
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