Insulin Protein (INS) (AA 25-54, AA 90-110) (His tag)
INS
宿主: 小鼠
宿主: 大肠杆菌（E. Coli）
> 97 %
AcA, CC
Active
2 images
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- 抗原 See all Insulin (INS) 蛋白
- Insulin (INS)
- 生物活性
- Active
- 产品特性
- AA 25-54, AA 90-110
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宿主
- 小鼠
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资源
- 大肠杆菌（E. Coli）
- 标记
- This Insulin protein is labelled with His tag.
- 应用范围
- Activity Assay (AcA), Cell Culture (CC)
- 产品特性
- Tag location: N-terminal His Tag
- 纯度
- > 97 %
- Biological Activity Comment
- INS (Insulin) is a peptide hormone produced by beta cells of the pancreatic islets, which decreases blood glucose concentration and increases cell permeability to monosaccharides, amino acids and fatty acids. It has been reported that insulin triggers phosphorylation of a number of substrates by binding to its receptors, which was important for cell proliferation, cell cycle progression, cell division and differentiation. To detect the effect of Insulin on cell proliferation, MCF-7 cells were seeded into triplicate wells of 96-well plates at a density of 2,000 cells/well and allowed to attach overnight, then the medium was replaced with serum-free standard DMEM prior to the addition of various concentrations of INS. After incubated for 48h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10μL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37°C.
- Top Product
- Discover our top product INS 蛋白
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Insulin (INS) (AA 1-110) protein (GST tag)
INS 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Insulin (INS) proteinINS 宿主: 人 宿主: 酵母菌 Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
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- 应用备注
- Isoelectric Point: 7.1
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 缓冲液
- 20 mM Tris, 150 mM NaCl, pH 8.0, containing 1 mM EDTA, 1 mM DTT, 0.01 % SKL, 5 % Trehalose and Proclin300.
- 储存液
- Dithiothreitol (DTT), Other preservative, ProClin
- 注意事项
- This product contains ProClin and Dithiothreitol (DTT): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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- 抗原
- Insulin (INS)
- Abstract
- INS 产品
- 别名
- IDDM2 Protein, ILPR Protein, IRDN Protein, MODY10 Protein, ins1 Protein, xins Protein, ins1-a Protein, Insulin Protein, AA986540 Protein, Ins-2 Protein, InsII Protein, Mody Protein, Mody4 Protein, proinsulin Protein, zgc:109842 Protein, igf2-A Protein, ins Protein, ins-a Protein, ins-b Protein, insulin Protein, insulin precursor Protein, insulin II Protein, preproinsulin Protein, insulin L homeolog Protein, insulin S homeolog Protein, INS Protein, INS-IGF2 Protein, ins Protein, Ins Protein, PIN Protein, Ins2 Protein, ins.L Protein, ins.S Protein
- 背景
- Alternative Names: Insulin
- 分子量
- 10kDa
- UniProt
- P01315
- 途径
- NF-kappaB Signaling, RTK signaling, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Hormone Activity, Carbohydrate Homeostasis, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Autophagy, Negative Regulation of intrinsic apoptotic Signaling, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation
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