TGFB1 Protein (AA 30-278) (His tag)
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- 抗原 See all TGFB1 蛋白
- TGFB1 (Transforming Growth Factor, beta 1 (TGFB1))
- 蛋白类型
- Recombinant
- 产品特性
- AA 30-278
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宿主
- 小鼠
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资源
- 大肠杆菌（E. Coli）
- 标记
- This TGFB1 protein is labelled with His tag.
- 应用范围
- SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys)
- 序列
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LSTCKTIDME LVKRKRIEAI RGQILSKLRL ASPPSQGEVP PGPLPEAVLA LYNSTRDRVA GESADPEPEP EADYYAKEVT RVLMVDRNNA IYEKTKDISH SIYMFFNTSD IREAVPEPPL LSRAELRLQR LKSSVEQHVE LYQKYSNNSW RYLGNRLLTP TDTPEWLSFD VTGVVRQWLN QGDGIQGFRF SAHCSCDSKD NKLHVEINGI SPKRRGDLGT IHDMNRPFLL LMATPLERAQ HLHSSRHRR
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - 产品特性
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- Made in Germany - from design to production - by highly experienced protein experts.
- Mouse Tgfb1 Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Two step purification of proteins expressed in bacterial culture:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Endotoxin has not been removed. Please contact us if you require endotoxin removal.
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product TGFB1 蛋白
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Transforming Growth Factor, beta 1 (TGFB1) (AA 30-278) protein (His-Avi Tag,Biotin)
TGFB1 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Transforming Growth Factor, beta 1 (TGFB1) proteinTGFB1 宿主: 人 宿主: HEK-293 Cells Recombinant > 95% as determined by Tris-Bis PAGE
Transforming Growth Factor, beta 1 (TGFB1) (AA 200-503) (Active) protein (GST tag)TGFB1 宿主: 人 宿主: Insect cells (Sf21) Recombinant SDS, Func Active
Transforming Growth Factor, beta 1 (TGFB1) (AA 279-390) protein (AVI tag,Biotin)TGFB1 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Transforming Growth Factor, beta 1 (TGFB1) (AA 30-390) (Active) protein (His tag)TGFB1 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Func Active
Transforming Growth Factor, beta 1 (TGFB1) (AA 30-390) (Active) protein (His tag,AVI tag,Biotin)TGFB1 宿主: 人 宿主: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
Transforming Growth Factor, beta 1 (TGFB1) (AA 30-278) (Active) protein (His tag,AVI tag,Biotin)TGFB1 宿主: 人 宿主: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
Transforming Growth Factor, beta 1 (TGFB1) (AA 281-390) protein (GST tag)TGFB1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Transforming Growth Factor, beta 1 (TGFB1) (AA 279-390) protein (GST tag)TGFB1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Transforming Growth Factor, beta 1 (TGFB1) (AA 30-278) protein (His tag)TGFB1 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- 说明
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Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- TGFB1 (Transforming Growth Factor, beta 1 (TGFB1))
- 别名
- Tgfb1 (TGFB1 产品)
- 别名
- CED Protein, DPD1 Protein, LAP Protein, TGFB Protein, TGFbeta Protein, TGF-beta Protein, TGF-BETA-1 Protein, TGF-beta5 Protein, ced Protein, dpd1 Protein, lap Protein, tgf-beta Protein, tgfb Protein, tgfb5 Protein, tgfbeta Protein, TGF-beta1 Protein, TGFbeta1 Protein, Tgfb Protein, Tgfb-1 Protein, ai39657 Protein, tgfb1 Protein, wu:fb13a07 Protein, xx:ai39657 Protein, TGFB1 Protein, csd Protein, cdb1 Protein, cdg2 Protein, csd1 Protein, csd2 Protein, csd3 Protein, ebmd Protein, lcd1 Protein, bigh3 Protein, cdgg1 Protein, betaig-h3 Protein, TGFB4 Protein, transforming growth factor beta 1 Protein, transforming growth factor beta-1 Protein, transforming growth factor beta 1 L homeolog Protein, transforming growth factor, beta 1 Protein, transforming growth factor, beta 1a Protein, transforming growth factor beta induced L homeolog Protein, TGFB1 Protein, Tgfb1 Protein, tgfb1.L Protein, tgfb1a Protein, tgfbi.L Protein
- 背景
- Multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts. Can promote either T-helper 17 cells (Th17) or regulatory T-cells (Treg) lineage differentiation in a concentration-dependent manner. At high concentrations, leads to FOXP3-mediated suppression of RORC and down-regulation of IL-17 expression, favoring Treg cell development. At low concentrations in concert with IL-6 and IL-21, leads to expression of the IL-17 and IL-23 receptors, favoring differentiation to Th17 cells (PubMed:18368049). Mediates SMAD2/3 activation by inducing its phosphorylation and subsequent translocation to the nucleus. Can induce epithelial-to-mesenchymal transition (EMT) and cell migration in various cell types (By similarity). {ECO:0000250|UniProtKB:P01137, ECO:0000269|PubMed:18368049, ECO:0000269|PubMed:22781750}.
- 分子量
- 29.4 kDa Including tag.
- UniProt
- P04202
- 途径
- EGFR Signaling Pathway, Dopaminergic Neurogenesis, Cellular Response to Molecule of Bacterial Origin, Glycosaminoglycan Metabolic Process, Regulation of Leukocyte Mediated Immunity, Regulation of Muscle Cell Differentiation, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Production of Molecular Mediator of Immune Response, Ribonucleoside Biosynthetic Process, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy, Cancer Immune Checkpoints
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