p21 Protein (AA 2-164) (His tag)
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- 抗原 See all p21 (CDKN1A) 蛋白
- p21 (CDKN1A) (Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A))
- 蛋白类型
- Recombinant
- 产品特性
- AA 2-164
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宿主
- 人
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资源
- Insect Cells
- 标记
- This p21 protein is labelled with His tag.
- 应用范围
- SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys)
- 序列
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SEPAGDVRQN PCGSKACRRL FGPVDSEQLS RDCDALMAGC IQEARERWNF DFVTETPLEG DFAWERVRGL GLPKLYLPTG PRRGRDELGG GRRPGTSPAL LQGTAEEDHV DLSLSCTLVP RSGEQAEGSP GGPGDSQGRK RRQTSMTDFY HSKRRLIFSK RKP
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - 产品特性
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- Made in Germany - from design to production - by highly experienced protein experts.
- Human CDKN1A Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- 纯化方法
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- 纯度
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Protein is endotoxin free.
- 质量等级
- Crystallography grade
- Top Product
- Discover our top product CDKN1A 蛋白
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Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A) (AA 2-164) protein (His tag)
CDKN1A 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A) (AA 2-164) protein (His-IF2DI Tag)CDKN1A 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A) (AA 1-164) protein (GST tag)CDKN1A 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A) (AA 65-164) protein (GST tag)CDKN1A 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A) (AA 2-159) protein (His tag)Crystallography grade CDKN1A 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)CDKN1A 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- 说明
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In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- 注意事项
- Avoid repeated freeze-thaw cycles.
- 储存条件
- -80 °C
- 储存方法
- Store at -80°C.
- 有效期
- Unlimited (if stored properly)
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- 抗原
- p21 (CDKN1A) (Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A))
- 别名
- CDKN1A (CDKN1A 产品)
- 别名
- CCND1 Protein, CDKN1A Protein, CAP20 Protein, CDKN1 Protein, CIP1 Protein, MDA-6 Protein, P21 Protein, SDI1 Protein, WAF1 Protein, p21CIP1 Protein, CDKI Protein, Cdkn1 Protein, Waf1 Protein, mda6 Protein, p21Cip1 Protein, p21WAF Protein, Cip1 Protein, UV96 Protein, p21 Protein, cip1 Protein, p16Xic2 Protein, sdi1 Protein, waf1 Protein, cap20 Protein, cdkn1 Protein, mda-6 Protein, p21cip1 Protein, si:ch1073-48m5.2 Protein, KRAS2 Protein, p21ras Protein, cyclin D1 Protein, cyclin dependent kinase inhibitor 1A Protein, cyclin-dependent kinase inhibitor 1A (P21) Protein, cyclin-dependent kinase inhibitor 1A Protein, cyclin-dependent kinase inhibitor 1A L homeolog Protein, cyclin-dependent kinase inhibitor 1 Protein, KRAS proto-oncogene, GTPase Protein, CCND1 Protein, CDKN1A Protein, Cdkn1a Protein, cdkn1a.L Protein, cki1 Protein, cdkn1a Protein, KRAS Protein
- 背景
- May be the important intermediate by which p53/TP53 mediates its role as an inhibitor of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex. {ECO:0000269|PubMed:8242751, ECO:0000269|PubMed:9106657}.
- 分子量
- 18.9 kDa Including tag.
- UniProt
- P38936
- 途径
- p53 Pathway, PI3K-Akt Signaling, Cell Division Cycle, AMPK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Mitotic G1-G1/S Phases, DNA Replication, Hepatitis C, Synthesis of DNA, Autophagy
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