CTLA4 Protein (AA 37-162) (Fc Tag,AVI tag,Biotin)
(1 reference)-
- 抗原 See all CTLA4 蛋白
- CTLA4 (Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4))
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- AA 37-162
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宿主
- 人
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资源
- HEK-293 Cells
- 标记
- This CTLA4 protein is labelled with Fc Tag,AVI tag,Biotin.
- 品牌
- MABSol®,PrecisionAvi
- 序列
- AA 37-162
- 特异性
- Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
- 产品特性
- This protein carries a human IgG1 Fc fragment at the C-terminus, followed by an Avi tag (Avitag™). The protein has a calculated MW of 42 kDa. The protein migrates as 50-55 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation.
- 纯度
- >95 % as determined by reduced SDS-PAGE.
- 内毒素水平
- Less than 1.0 EU per μg by the LAL method.
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- Discover our top product CTLA4 蛋白
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Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4) (AA 36-161) protein (Fc Tag)
CTLA4 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4) protein (mFc-His Tag)CTLA4 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. ELISA, FACS
Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4) (AA 36-161) protein (His tag)CTLA4 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4) protein (His-Avi Tag,Biotin)CTLA4 宿主: 人 宿主: HEK-293 Cells Recombinant > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4) (AA 37-160) protein (His tag)CTLA4 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4) (AA 37-162) (Active) protein (His tag)CTLA4 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4) (AA 37-160) (Active) protein (Fc Tag)CTLA4 宿主: 人 宿主: HEK-293 Cells Recombinant >98 % as determined by SDS-PAGE. Active
Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4) (AA 36-162) (Active) protein (His tag)CTLA4 宿主: 小鼠 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4) (Active) protein (His tag)CTLA4 宿主: 小鼠 宿主: 人细胞 Recombinant > 95 % as determined by SDS-PAGE Active
Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4) (Active) protein (His tag)CTLA4 宿主: 豚鼠 宿主: Yeast (Pichia pastoris) Recombinant > 90 % as determined by reducing SDS-PAGE. Active
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- 说明
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Ready-to-use AvitagTM biotinylated protein:
The product is exclusively produced using the AvitagTM technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.
This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled. - 限制
- 仅限研究用
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- 状态
- Lyophilized
- 缓冲液
- Tris with Glycine, Arginine and NaCl, pH 7.5
- 注意事项
- Please avoid repeated freeze-thaw cycles.
- 储存条件
- -20 °C
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: "Revealing the atomistic details behind the binding of B7-1 to CD28 and CTLA-4: A comprehensive protein-protein modelling study." in: Biochimica et biophysica acta. General subjects, Vol. 1862, Issue 12, pp. 2764-2778, (2019) (PubMed).
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: "Revealing the atomistic details behind the binding of B7-1 to CD28 and CTLA-4: A comprehensive protein-protein modelling study." in: Biochimica et biophysica acta. General subjects, Vol. 1862, Issue 12, pp. 2764-2778, (2019) (PubMed).
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- 抗原
- CTLA4 (Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4))
- 别名
- CTLA-4 (CTLA4 产品)
- 别名
- CTLA-4 Protein, CTLA4 Protein, CD152 Protein, Cd152 Protein, Ctla-4 Protein, Ly-56 Protein, CD Protein, CELIAC3 Protein, GRD4 Protein, GSE Protein, IDDM12 Protein, sCTLA4 Protein, cytotoxic T-lymphocyte associated protein 4 Protein, cytotoxic T-lymphocyte-associated protein 4 Protein, CTLA4 Protein, Ctla4 Protein
- 背景
- CTLA-4 (Cytotoxic T-Lymphocyte Antigen 4) is also known as CD152 (Cluster of differentiation 152), is a protein receptor that downregulates the immune system. CTLA4 is a member of the immunoglobulin superfamily, which is expressed on the surface of Helper T cells and transmits an inhibitory signal to T cells. The protein contains an extracellular V domain, a transmembrane domain, and a cytoplasmic tail. Alternate splice variants, encoding different isoforms. CTLA4 is similar to the T-cell co-stimulatory protein, CD28, and both molecules bind to CD80 and CD86, also called B7-1 and B7-2 respectively, on antigen-presenting cells. CTLA4 transmits an inhibitory signal to T cells, whereas CD28 transmits a stimulatory signal. Intracellular CTLA4 is also found in regulatory T cells and may be important to their function. Fusion proteins of CTLA4 and antibodies (CTLA4-Ig) have been used in clinical trials for rheumatoid arthritis.
- 分子量
- 41.8 kDa
- NCBI登录号
- NP_005205
- 途径
- Cancer Immune Checkpoints
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