Optimal working dilution should be determined by the investigator.,Optimal working dilution should be determined by the investigator.
限制
仅限研究用
状态
Lyophilized
溶解方式
Prior to use, reconstitute the lyophilized powder with 0.2 mLof PBS containing a carrier protein (e.g., 1 % BSA, protease free), pH 7.4. Re-cap vial, vortex. Allow the reconstituted standard to sit at room temperature for 15 minutes, vortex again to mix completely. The reconstituted standard stock solution can be aliquoted into polypropylene vials and stored at -70 °C for up to one month.
缓冲液
Lyophilized in sterile-filtered PBS, pH 7.2, containing 1 % BSA.
注意事项
Use a manual defrost freezer and avoid repeated freeze thaw cycles.
储存条件
4 °C
储存方法
Upon receipt, store unopened vials between 2°C and 8°C immediately and use within 12 months from date of receipt.
Bioactive IL-12 is a potent regulator of cell-mediated immune responses and key mediator of Th1 cell development. Bioactive IL-12 is secreted by activated B lymphocytes, dendritic cells, and macrophages as a 70 kD heterodimeric glycoprotein comprised of disulfide-bonded 35 kD and 40 kD subunits. The disulfide-linked p40 homodimer can bind to IL-12 receptors and antagonize the activities of bioactive IL-12. Recombinant human IL-12 is a 70 kD protein containing a 306 amino acid p40 subunit and a 197 amino acid p35 subunit.